FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685330

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685330
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026374
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA60090.5854591016530037No Hit
GTATATTACTGTGCGAAGACCGCGACTAACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTC37540.3657536141796265No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT29750.2898553548706417No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC28860.2811840518173687No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25910.2524420922587673No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24960.24318620697718374No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT24040.23422261280975548No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATCA22670.2208746519299982No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22640.22058236081584295No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG22230.21658771558905426No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21840.21278793110503577No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG19960.19447102128463892No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAG19320.1882354775159932No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT19280.18784575603045284No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGGCTAGAGACTGAAGATTTTGCAGTTTATTACTGTCAGCAGCG19210.1871637434307572No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18810.18326652857535364No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18770.18287680708981324No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18590.18112306040488163No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG18310.17839501000609914No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18090.17625154183562716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17590.1713800232663727No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17310.16865197286759018No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG16620.161929277242019No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATTAGCAGTCGCTTAGCCTGGTA16370.15949351795739175No Hit
CTCCTGCAGGGCCAGTCAGACTATTAGCAGTCGCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC16260.15842178387215577No Hit
CAGTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATA16230.1581294927580005No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16210.15793463201523034No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC16070.15657060681583906No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15690.15286825270320567No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15500.15101707564688896No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCC15490.15091964527550386No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15110.14721729116287044No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG15100.14711986079148537No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14980.1459506963348643No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14750.14370979779300722No Hit
GACTATTAGCAGTCGCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC14730.14351493705023705No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14450.14078688665145453No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG14440.14068945628006943No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14440.14068945628006943No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC14340.13971515256621855No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG14200.1383511273668273No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14140.13776654513851677No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGTG13800.13445391251142372No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13780.13425905176865355No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13650.13299245694064737No Hit
GACTTCACTCTCACCATCAGCAGGCTAGAGACTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAGC13430.1308489887701754No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13420.1307515583987903No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13390.13045926728463503No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGGCCCCAGAGATCGAAGTACCAGTTAG13320.12977725468493942No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13290.12948496357078415No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC13050.12714663465754197No Hit
GGGCTGGAGTGGGTCTCAAGTATTGGCGGTAATGGTGGTAACACATTCTA12950.1261723309436911No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT12520.12198282497413224No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12490.12169053385997697No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGG12440.12120338200305153No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTG12400.12081366051751116No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT12280.11964449606089009No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA12280.11964449606089009No Hit
GTATCAACGCAGAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGAT12110.11798817974734356No Hit
GTTTATTACTGTCAGCAGCGTAGCAGCTGGCCGCTCACTTTCGGCGGAGGGGCCAAGGTGGAGATCAAACGAA11960.11652672417656722No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTTAGCAGCTATGCC11630.11331152192085925No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGA11610.11311666117808908No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT11530.11233721820700837No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGGGCTTCTTCCCCCAGGAGCCACTCAG11460.11165520560731274No Hit
GCGTAGCAGCTGGCCGCTCACTTTCGGCGGAGGGGCCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA11380.11087576263623203No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGCTGC11360.11068090189346184No Hit
GTAATAAACTGCAAAATCTTCAGTCTCTAGCCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA11290.10999888929376621No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT11270.10980402855099602No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGGTGGTGGAG11260.10970659817961094No Hit
ATATTACTGTGCGAAGACCGCGACTAACTGGTACTTCGATCTCTGGGGCC11090.10805028186606441No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGG11080.10795285149467933No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACAGTGACCAGGGTGCCACGG11040.10756313000913897No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGCCCCTCCGCCGAAAGTGAGCGGCCAG11020.1073682692663688No Hit
CCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG10970.10688111740944334No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10830.1055170922100521No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC10730.10454278849620119No Hit
GAGTACGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT10650.10376334552512048No Hit
GGGTGCCACGGCCCCAGAGATCGAAGTACCAGTTAGTCGCGGTCTTCGCACAGTAATATACGGCCGTGTCCTCGG10570.10298390255403976No Hit
GCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAG10510.10239932032572922No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10460.10191216846880376No Hit
GAACTGGGCAGGGCACAGTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGG10350.1008404343835678No Hit
GCCGTATATTACTGTGCGAAGACCGCGACTAACTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCA10330.10064557364079761No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10300.10035328252664233No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.002421194568.5227539
TGTTAGA6650.047.07343770
TCCGGAG29450.045.94680870
ATGCCGT1200.045.8370644
GTGTATA452.0493135E-645.361241
GTGGTAT1900.044.7643851
GGTATCA7600.044.3167421
CCCACGA2400.043.93319
CGTATGC1250.043.9170741
CTTATAC2500.043.5467951
ATCTCGT1500.043.35305837
TCGTATG1200.042.85827340
GCCGTCT1050.042.63647546
CACGAGA2400.042.52616521
TAGGCAA2850.042.5179470
TGGTATC2100.042.104742
CTTGAAA1500.041.76966557
TACACAT2550.041.3446775
TGTATAA504.2413303E-640.8092082
ACACATC2600.040.5495836
Produced by FastQC (version 0.11.5)