FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713190
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT40000.5608603597919208No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC36380.510102497230752No Hit
CCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTAGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTT30940.43382548829905077No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27750.389096874605645No Hit
GTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAAC27040.3791416032193385No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT26950.37787966740980666No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTC26920.3774590221399627No Hit
GGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGTTCTAA25700.36035278116630914No Hit
GGTCTAGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTC24400.34212481947307166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23760.33315105371640097No Hit
TTATTATTGCATGCAATCTCTAGAAACTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCGAACTG23220.32557943885921004No Hit
GAGTAGTACCCATTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGAT22530.3159045976527994No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22450.31478287693321555No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG22400.31408180148347564No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC22120.3101557789649322No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20590.2887028702028912No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20560.28828222493304734No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20150.28253340624518014No Hit
GTGCAGCCACAGTTCGGTTAATCTCCAGTCGTGTCCCTTGGCCGAACGTG20000.2804301798959604No Hit
CAATAATAAAGTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCGTAAAATCTGTGTCTGACCCACTGCCA19770.27720523282715687No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19610.27496179138798915No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA19570.27440093102819724No Hit
TGATTAGAGTTTCTAGAGATTGCATGCAATAATAAAGTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCG19140.2683716821604341No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18800.26360436910220275No Hit
GTCAGGGACCCCGAGGGCCCGATTAGAACCCAAATACAATAGGAGTTGTGGAGACTGCCCCGTCTTCTGCAAATA18300.2565936146048038No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTAATCTCCAGTCGTGTCCCTTGGCCGAACGTGATTAGAGTT18270.2561729693349598No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT17930.2514056562767285No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC17930.2514056562767285No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17850.2502839355571447No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGA17670.247760063938081No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCAGACACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGA17570.24635791303860125No Hit
GTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTAGTCAGAGCCTCCTGCA17110.23990801890099414No Hit
GCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCC16580.23247661913375117No Hit
CAATAGGAGTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAG16230.22756909098557185No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCC15680.21985726103843298No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC15410.2160714536098375No Hit
GTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGC15160.212566076361138No Hit
CTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTA14570.20429338605420713No Hit
GTTCAGTGGCAGTGGGTCAGACACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGGACTTTA14530.20373252569441522No Hit
CTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGACAGGTTCAGTGGCAGTGGGTCAGACACAGAT14050.19700220137691218No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13730.1925153184985768No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT13550.18999144687951317No Hit
CTCCAGGGGTGACGGCCAGGGAGAGTGGAGACTGAGTCAGCACAATGTCCCCACTGGAGCCAGAGACCCAGAGCA13440.1884490808900854No Hit
CTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC13430.18830886580013742No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCAC13420.18816865071018943No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13320.18676649981070964No Hit
GCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATT13310.18662628472076165No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13280.1862056394509177No Hit
GCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGT13240.18564477909112578No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13140.184242628191646No Hit
GACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGCCAGGGAGAGTGGAGACTGAGTCAGCACAA13090.1835415527419061No Hit
GGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATC12990.18213940184242627No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12710.17821337932388284No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12670.17765251896409093No Hit
GTAGTACCCATTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGC12630.17709165860429898No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12400.17386671153549546No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACAT12340.1730254209958076No Hit
GTCGTGTCCCTTGGCCGAACGTGATTAGAGTTTCTAGAGATTGCATGCAATAATAAAGTCCAACATCCTCAGCC12180.1707819795566399No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12000.16825810793757626No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11800.16545380613861665No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11530.16166799871002116No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT11480.16096692326028125No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11300.1584430516412176No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11220.15732133092163378No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11140.15619961020204995No Hit
CCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAG11070.15521810457241408No Hit
GTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGACAGGTTCAGTGGCA10760.1508714367840267No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGGTTAATCTCCAGTCG10620.14890842552475497No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG10300.1444215426464196No Hit
GTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGA10120.14189767102735595No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10090.14147702575751203No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9990.14007487485803222No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC9930.13923358431834434No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9910.1389531541384484No Hit
AGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAA9880.13853250886860444No Hit
TTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGCCAGGGAGAG9750.1367097126992807No Hit
GTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAGAGTAGTACCCATTGCTATGCAGGAGGCTCTGA9690.13586842215959283No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT9610.13474670144000897No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9530.1336249807204251No Hit
CTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTG9480.13292390527068523No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG9440.13236304491089332No Hit
CGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA9400.13180218455110138No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT9290.1302598185616736No Hit
CCCAAATACAATAGGAGTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAGAGTAGTACCCATTGCTA9270.12997938838177764No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9270.12997938838177764No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT9260.12983917329182967No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTC9250.1296989582018817No Hit
CTCCACAACTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCT9250.1296989582018817No Hit
CCACTGAACCTGTCAGGGACCCCGAGGGCCCGATTAGAACCCAAATACAATAGGAGTTGTGGAGACTGCCCCG9140.1281565922124539No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA9070.12717508658281806No Hit
GCCAAGGGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTC8980.12591315077328621No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT8970.12577293568333825No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC8860.12423056969391044No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC8830.1238099244240665No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8830.1238099244240665No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC8830.1238099244240665No Hit
CTAGAAACTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCG8790.1232490640642746No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC8760.12282841879443067No Hit
GTTAATCTCCAGTCGTGTCCCTTGGCCGAACGTGATTAGAGTTTCTAGAG8750.12268820370448268No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG8730.1224077735245867No Hit
GAGTGGAGGCTGAGGATGTTGGACTTTATTATTGCATGCAATCTCTAGAAACTCTAATCACGTTCGGCCAAGGGA8700.12198712825474277No Hit
CAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAG8630.12100562262510692No Hit
GTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGG8530.11960347172562712No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG8520.11946325663567912No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC8500.11918282645578317No Hit
CCACAACTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGA8490.11904261136583519No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8480.11890239627588722No Hit
GGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGG8420.11806110573619932No Hit
GACACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGG8320.11665895483671952No Hit
GATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGGACTTTATTATTGCATGCAATCTCTAGAAAC8170.11455572848749983No Hit
ATGTTGGACTTTATTATTGCATGCAATCTCTAGAAACTCTAATCACGTTC8140.11413508321765588No Hit
GTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCGTAAAATCTGTGTCTGACCCACTGCCACTGAAC8110.11371443794781194No Hit
GTCTCCACAACTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCC8100.11357422285786396No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA8070.11315357758802003No Hit
ATCTGTGTCTGACCCACTGCCACTGAACCTGTCAGGGACCCCGAGGGCCCGATTAGAACCCAAATACAATAGGAG7990.11203185686843618No Hit
GCCTCCACTCTGGCGATTGTTAGCGTAAAATCTGTGTCTGACCCACTGCC7960.11161121159859223No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT7950.11147099650864425No Hit
CTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTG7930.1111905663287483No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC7900.11076992105890436No Hit
ATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGCCAGGGAGAGTGGAGA7890.11062970596895638No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG7890.11062970596895638No Hit
CTGCAAATACCAATCCAAAGAGTAGTACCCATTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGC7870.11034927578906042No Hit
ACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATC7810.10950798524937255No Hit
GTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA7690.10782540416999678No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC7680.1076851890800488No Hit
GAGTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAGAGTAGT7610.10670368345041294No Hit
GGGTCAGACACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGGACTTTATTATTGCATGCAA7540.10572217782077707No Hit
AGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGACAGGTTCAG7470.10474067219114121No Hit
GAGTTTCTAGAGATTGCATGCAATAATAAAGTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCGTAAAA7400.10375916656150536No Hit
TCCTCAGCCTCCACTCTGGCGATTGTTAGCGTAAAATCTGTGTCTGACCCACTGCCACTGAACCTGTCAGGGACC7310.10249723075197352No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC7270.10193637039218162No Hit
ACCCATTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCC7200.10095486476254575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)