FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205665
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGCTTGGCCGTATTAGAAGTGAAGCGCAGAGT59880.49665537276109034No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTG42300.3508437252470628No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG38250.31725230474468447No Hit
GTGTATAGCAGAGCCACTGAATGTGAACCCAGAGGCAGCACAGGAGAGTT37980.3150128767111926No Hit
GCCTTGGGCTGACCTAGGACGGACACCTTGGTCCCTCCGCCAAATAATATTGTGCTGAATTTTGTATATGAGC29150.24177528583810592No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28770.23862349823541365No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT27960.231905214134938No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG27820.23074402922868292No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27720.22991461143850073No Hit
CTCCAGAGATGATTCAAGGAACATGGCGTATCTGCAAATGAACAATCTCAAAATCGAGGACACGGCCGTGTATTA26380.2188004130500595No Hit
TCTCTGGAGAGGGTGAACCTGCCTCTCACCGACTCAGCAAATACTGTCGCGTAACTCTGCGCTTCACTTCTAATA25180.20884739956787335No Hit
GATTATTACTGCAGCTCATATACAAAATTCAGCACAATATTATTTGGCGGAGGGACCAAGGTGTCCGTCCTAGG24990.2072715057665272No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG23980.19889438608568713No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23530.19516200602986733No Hit
GTATTTGCTGAGTCGGTGAGAGGCAGGTTCACCCTCTCCAGAGATGATTC23310.19333728689146654No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGACACCTTGGTCCCTC22870.18968784861466492No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT22250.18454545831553543No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21970.1822230885030253No Hit
GTCCTGGGCCCAATCTGCCCTGAGTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC21780.18064719470167917No Hit
GACCTAGGACGGACACCTTGGTCCCTCCGCCAAATAATATTGTGCTGAATTTTGTATATGAGCTGCAGTAATAA21600.17915424267935123No Hit
ATATTATTTGGCGGAGGGACCAAGGTGTCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCG21460.17799305777309618No Hit
GAGTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACAT21010.17426067771727635No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACATTGGTGGCTATAAGTATGTCTCCTGGT20910.17343125992709416No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20900.17334831814807594No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGAGTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCAC20500.17003064698734724No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20490.16994770520832903No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA20340.16870357852305573No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19160.158916448598906No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC19090.15833585614577847No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18800.15593054455425015No Hit
GTAATACACGGCCGTGTCCTCGATTTTGAGATTGTTCATTTGCAGATACG18600.15427170897388576No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG18430.1528616987305761No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCCGAGATGGTCAGGGAGG18360.15228110627744854No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18240.15128580492922994No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG18210.15103697959217527No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC17630.14622635640911863No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17460.1448163461658089No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17460.1448163461658089No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17390.14423575371268138No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG17280.14332339414348097No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16870.13992278120373405No Hit
CTGCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGCTT16770.13909336341355186No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT16590.13760041139122395No Hit
GGGCTGGAGTGGCTTGGCCGTATTAGAAGTGAAGCGCAGAGTTACGCGAC16560.13735158605416928No Hit
CCTTGGTCCCTCCGCCAAATAATATTGTGCTGAATTTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCGTCAG16470.1366051100430053No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16060.13320449710325838No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16000.13270684642914904No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15840.13137977796485759No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15770.13079918551173003No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG15740.1305503601746754No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15600.12938917526842034No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15590.12930623348940212No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15440.12806210680412883No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15200.1260715041076916No Hit
AAATAATATTGTGCTGAATTTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCCGAGA15150.1256567952126005No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15130.12549091165456408No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14910.12366619251616329No Hit
CTCCTGCACTGGAACCAACAGTGACATTGGTGGCTATAAGTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGC14870.1233344254000904No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14820.12291971650499932No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG14800.12275383294696286No Hit
CTCCTGTGCTGCCTCTGGGTTCACATTCAGTGGCTCTGCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGGG14630.12134382270365318No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGTTTCCCGGGCCCCAGTAGTCCCCCATTGGAGCTC14620.12126088092463497No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCTCCAGCCTTGGGATTCCCAGCTGT14550.12068028847150743No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC14330.11885556933310662No Hit
CAGCAAATACTGTCGCGTAACTCTGCGCTTCACTTCTAATACGGCCAAGCCACTCCAGCCCTTTCCCGGAAGC14200.1177773262058698No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCTCCAGCCTTGGGATTCCCAGCTGTCTCCCCTCGGTGA14180.11761144264783335No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14180.11761144264783335No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAG14170.11752850086881514No Hit
GAGTTACGCGACAGTATTTGCTGAGTCGGTGAGAGGCAGGTTCACCCTCT14120.11711379197372404No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG14110.11703085019470583No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13990.11603554884648722No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT13860.11495730571925036No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACATTGGTGGCTATAAGTATGTCTCCTGGTTCCAACAGCA13690.11354729547594067No Hit
GTGTATTACTGCACTAGATGGATGGAAGTGGGAGCTCCAATGGGGGACTA13540.11230316879066739No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13540.11230316879066739No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGAGTCAGC13380.11097610032637589No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT13370.11089315854735768No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT13320.11047844965226661No Hit
CTTCTAATACGGCCAAGCCACTCCAGCCCTTTCCCGGAAGCCTGGCGGACCCAGTGTATAGCAGAGCCACTGAA13290.11022962431521194No Hit
GTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGCCCCCAAAATCATCA13160.1091513811879751No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG13050.1082390216187747No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT12880.106829011375465No Hit
GTACGGGAGCTCTGGGAGAGGAGCTCCAGCCTTGGGATTCCCAGCTGTCT12720.1055019429111735No Hit
GCACTGAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12560.104174874446882No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC12390.10276486420357231No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12280.1018525046343719No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12270.10176956285535368No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12220.1013548539602626No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGTTTCCCGGG12170.1009401450651715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG3050.087.6822770
GACGAAG11350.077.28470
GCGCAAG450.00206554671.3149170
GTGGTAT1700.052.068721
CGGGTTA200.007767201451.0483417
TGGTATC1900.046.5704152
GGTATCA7400.044.16641
GGACGAA11350.041.26178769
GTATCAA19450.039.38361
TCGTATG2600.038.27170640
CTCTTAT1250.038.1303251
CAAATGA18000.038.0346270
CCGATCA2901.8553692E-1036.88702470
CGTATGC2850.036.14418441
TTATACA2950.035.762682
CTTATAC3000.035.1797641
CCCTAAG2950.034.6492828
TCTCGCG14850.034.57692370
TATCAAC22100.034.4941982
GTGGCCG19700.034.20943570
Produced by FastQC (version 0.11.5)