FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1205665
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGCTTGG55970.46422513716496705No Hit
GAATACAGGAGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGT42430.3519219683742997No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT37880.3141834589210104No Hit
GTGTATAGCAGAGCCACTGAATGTGAACCCAGAGGCAGCACAGGAGAGTTTCAGGGACCCCCCGGGCTGGACGAA35620.2954386168628931No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35090.29104270257492754No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27350.22684576561482667No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27300.22643105671973557No Hit
GCCTTGGGCTGACCTAGGACGGACACCTTGGTCCCTCCGCCAAATAATATTGTGCTGAATTTTGTATATGAGCT25860.21448744054111216No Hit
CTCCAGAGATGATTCAAGGAACATGGCGTATCTGCAAATGAACAATCTCAAAATCGAGGACACGGCCGTGTATTA25860.21448744054111216No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24390.20229499902543407No Hit
TCTCTGGAGAGGGTGAACCTGCCTCTCACCGACTCAGCAAATACTGTCGCGTAACTCTGCGCTTCACTTCTAATA23530.19516200602986733No Hit
GTATTTGCTGAGTCGGTGAGAGGCAGGTTCACCCTCTCCAGAGATGATTCAAGGAACATGGCGTATCTGCAAAT23370.19383493756557585No Hit
GATTATTACTGCAGCTCATATACAAAATTCAGCACAATATTATTTGGCGGAGGGACCAAGGTGTCCGTCCTAGGT23340.1935861122285212No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23080.19142962597404753No Hit
ATATTATTTGGCGGAGGGACCAAGGTGTCCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCC22240.18446251653651718No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22220.18429663297848076No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAACAGTGACATT22110.18338427340928035No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG22090.18321838985124392No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC21450.17791011599407797No Hit
GTCCTGGGCCCAATCTGCCCTGAGTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT21180.17567068796058605No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20560.17052829766145655No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG20340.16870357852305573No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20270.1681229860699282No Hit
GGGCACAGGGTCCTGGGCCCAATCTGCCCTGAGTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCAC19880.1648882566882177No Hit
GAGTCAGCCTGCCTCCATGTCTGGGTCTCCTGGACAGTCGATCACCATCT19760.1638929553399991No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19430.1611558766323979No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGACACCTTGGTCCCTC19420.16107293485337967No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18370.15236404805646675No Hit
GACCTAGGACGGACACCTTGGTCCCTCCGCCAAATAATATTGTGCTGAATTTTGTATATGAGCTGCAGTAATAAT18350.15219816449843032No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18310.15186639738235744No Hit
CTCCTGTGCTGCCTCTGGGTTCACATTCAGTGGCTCTGCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGG17930.14871460977966516No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG17910.14854872622162874No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17900.14846578444261052No Hit
GTAATACACGGCCGTGTCCTCGATTTTGAGATTGTTCATTTGCAGATACGCCATGTTCCTTGAATCATCTCTGGA17800.14763636665242832No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA17790.1475534248734101No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17570.1457287057350093No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16980.14083514077293444No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG16550.13726864427515106No Hit
CTGCTATACACTGGGTCCGCCAGGCTTCCGGGAAAGGGCTGGAGTGGCTTGGCCGTATTAGAAGTGAAGCGCAGA16530.1371027607171146No Hit
CTCCTGCACTGGAACCAACAGTGACATTGGTGGCTATAAGTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGC15770.13079918551173003No Hit
GGGCTGGAGTGGCTTGGCCGTATTAGAAGTGAAGCGCAGAGTTACGCGAC15730.13046741839565718No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCT15660.12988682594252965No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC15600.12938917526842034No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15590.12930623348940212No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15130.12549091165456408No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG15070.12499326098045477No Hit
GTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCCGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA15050.12482737742241833No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14970.12416384319027259No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGGT14530.120514404913471No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC14520.12043146313445276No Hit
CCTTGGTCCCTCCGCCAAATAATATTGTGCTGAATTTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCGTCA14240.11810909332194265No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC14200.1177773262058698No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG14150.11736261731077868No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAGGAGCTCCAGCCTTGGGATTCCCAGCTGTCTCCCCTCGGT14070.11669908307863296No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGAGTCAGC13880.11512318927728682No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAGCCGGCAAGTCCGTGACATGCCACGT13860.11495730571925036No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG13840.11479142216121394No Hit
GTCGATCACCATCTCCTGCACTGGAACCAACAGTGACATTGGTGGCTATAAGTATGTCTCCTGGTTCCAACAGCA13810.11454259682415928No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13790.11437671326612285No Hit
GTATGTCTCCTGGTTCCAACAGCACCCAGGCAAAGCCCCCAAAATCATCA13620.11296670302281314No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13470.11172257633753986No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG13380.11097610032637589No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATC13370.11089315854735768No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13370.11089315854735768No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG13330.11056139143128482No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC13250.10989785719913907No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG13230.10973197364110264No Hit
GAGTACGGGAGCTCTGGGAGAGGAGCTCCAGCCTTGGGATTCCCAGCTGT13230.10973197364110264No Hit
CAGCAAATACTGTCGCGTAACTCTGCGCTTCACTTCTAATACGGCCAAGCCACTCCAGCCCTTTCCCGGAAGC13210.10956609008306618No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT13190.10940020652502976No Hit
GAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGGTGGAGT13170.10923432296699331No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT13040.10815607983975649No Hit
AAATAATATTGTGCTGAATTTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCGTCAGCCTGGAGCCCCGAGAT13000.1078243127236836No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAG12970.10757548738662896No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCTTCGTCCAGCCCGGGGGGTCCCTGAAACTCTCCTGTGCTGC12950.10740960382859253No Hit
GTGTATTACTGCACTAGATGGATGGAAGTGGGAGCTCCAATGGGGGACTACTGGGGCCCGGGAAACCTGGTCACC12920.10716077849153786No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT12810.10624841892233747No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT12690.10525311757411886No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGTTTCCCGGGCCCCAGTAGTCCCCCATTGGAGCTC12550.10409193266786379No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12390.10276486420357231No Hit
GAGTTACGCGACAGTATTTGCTGAGTCGGTGAGAGGCAGGTTCACCCTCTCCAGAGATGATTCAAGGAACATGGC12370.10259898064553585No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG12340.10235015530848121No Hit
CCCCAGTAGTCCCCCATTGGAGCTCCCACTTCCATCCATCTAGTGCAGTAATACACGGCCGTGTCCTCGATTTT12240.10152073751829903No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)