FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004685346

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685346
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1539528
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGTCCCATGATGTCCCACCGTACACCTTTGGACAGGGGACCAAGGTGGAGATCAGACGAACTG73420.47689941332668195No Hit
CCTTTGGACAGGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCA62130.4035652485696915No Hit
GTCCAAAGGTGTACGGTGGGACATCATGGGACTGACAGTAATACACCGCAAAATCTTCAGGCTCTAGCCTGCTGA57700.3747901954365234No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT56580.3675152384367157No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC53270.34601514230335534No Hit
CAGTAATACACCGCAAAATCTTCAGGCTCTAGCCTGCTGACGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA46770.3037944097151854No Hit
ACCCTGGCCAGACTCCCAGGCTCCTCATTTATGCTGCCTCCCGCAGGGCCCCTGGCATCCCAGACAGGTTCAG37140.24124277051148146No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACTCCA36420.23656601244017647No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT36160.23487718313664968No Hit
GTCCCCAGACACCCTGTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCGTGCAGGGCCAGTCAGACCATTAACAA33030.21454627652111558No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32960.21409159170862757No Hit
GTGTATTACTGTCAGTCCCATGATGTCCCACCGTACACCTTTGGACAGGGGACCAAGGTGGAGATCAGACGAA32020.20798582422664608No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC31870.20701149962845758No Hit
GTCTGGGACAGACTTCACTCTCACCGTCAGCAGGCTAGAGCCTGAAGATTTTGCGGTGTATTACTGTCAGTCCCA31820.2066867247623947No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31220.20278942636964056No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG30730.19960663268222467No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC30440.19772293845906017No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT30360.19720329867335962No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27710.17999023077202883No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27700.17992527579881626No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG27270.1771322119506758No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC26990.17531347270072387No Hit
GTAATACACCGCAAAATCTTCAGGCTCTAGCCTGCTGACGGTGAGAGTGAAGTCTGTCCCAGACCCACCGCCA25970.16868806543304182No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25900.16823338062055385No Hit
CATTTATGCTGCCTCCCGCAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTCTGGGACAGACTTCA25650.1666095062902396No Hit
GTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTGCGGGAGGCAGCATAAATGAGGAGCC25620.16641464137060188No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTGCTACTGGTGGCAGCTCCCAGATGGGTC25430.1651804968795631No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24940.1619977031921472No Hit
GGGCCAGTCAGACCATTAACAACAACTACTTAGCCTGGTACCGACACAACCCTGGCCAGACTCCCAGGCTCCTCA24590.15972427912970727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT22850.1484221137907203No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT22740.147707609085382No Hit
GTCTTTGTCTCCAGGGGAAAGCGCCACCCTCTCGTGCAGGGCCAGTCAGACCATTAACAACAACTACTTAGCC22380.14536923004972954No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22200.14420004053190327No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21670.14075742695163712No Hit
GTAGTAGGTGTCCCCACTGTGTGAGATGAATCCAATCCACTCCAGGCCCTTCCCTGGGCGCTGGCGGATCCAGGC21430.13919850759453548No Hit
CTCCCAGGCTCCTCATTTATGCTGCCTCCCGCAGGGCCCCTGGCATCCCAGACAGGTTCAGTGGCGGTGGGTC21350.1386788678088349No Hit
TCATGGGACTGACAGTAATACACCGCAAAATCTTCAGGCTCTAGCCTGCT20950.13608066888033216No Hit
GAGTGAAGTCTGTCCCAGACCCACCGCCACTGAACCTGTCTGGGATGCCAGGGGCCCTGCGGGAGGCAGCATAAA20630.13400210973752996No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACTC20050.13023472129120092No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCC19980.12978003647871295No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19960.12965012653228783No Hit
GGTGTACGGTGGGACATCATGGGACTGACAGTAATACACCGCAAAATCTT19890.12919544171979983No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC19860.12900057680016214No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT19760.12835102706803642No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19760.12835102706803642No Hit
CTCTTAGACGTGTCAATGGAGATGGCGACTCGACTCTTGAGGGACGGGCT19730.12815616214839873No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT19510.12672715273772223No Hit
ACACAACCCTGGCCAGACTCCCAGGCTCCTCATTTATGCTGCCTCCCGCAGGGCCCCTGGCATCCCAGACAGGT19430.12620751295202168No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19410.12607760300559653No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC19170.12451868364849487No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19140.12432381872885716No Hit
GACTTCACTCTCACCGTCAGCAGGCTAGAGCCTGAAGATTTTGCGGTGTATTACTGTCAGTCCCATGATGTCCCA18770.12192048471999212No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18640.12107607006822871No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18450.1198419255771899No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA18260.11860778108615108No Hit
GCATAAATGAGGAGCCTGGGAGTCTGGCCAGGGTTGTGTCGGTACCAGGCTAAGTAGTTGTTGTTAATGGTCTGA18230.11841291616651337No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG18180.11808814130045053No Hit
GGCCAGGGTTGTGTCGGTACCAGGCTAAGTAGTTGTTGTTAATGGTCTGACTGGCCCTGCACGAGAGGGTGGCG17870.11607453713086088No Hit
GGGTTGTGTCGGTACCAGGCTAAGTAGTTGTTGTTAATGGTCTGACTGGCCCTGCACGAGAGGGTGGCGCTTTCC17860.11600958215764831No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA17850.11594462718443575No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA17460.11341138322914555No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGACTCCACCGGAGAAACTGTGCTGGCGCAGTCCC17350.1126968785238073No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG17000.11042345446136738No Hit
GTCCCATGATGTCCCACCGTACACCTTTGGACAGGGGACCAAGGTGGAGATCAGACGAACTGTGGCTGCACCA16960.1101636345685171No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16860.10951408483639141No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16850.10944912986317885No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16680.10834489531856518No Hit
GGCTAGAGCCTGAAGATTTTGCGGTGTATTACTGTCAGTCCCATGATGTCCCACCGTACACCTTTGGACAGGGGA16500.10717570580073893No Hit
GTCTCTGATGAGTCCATCGACAATTCTGATTACTACTGGGCCTGGATCCGCCAGCGCCCAGGGAAGGGCCTGGAG16420.10665606601503838No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16350.1062013812025504No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC16000.10392795714011048No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15990.10386300216689791No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15630.10152462313124543No Hit
GGACAATGGTCACCGTCTCTTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGC15630.10152462313124543No Hit
GTGCAGCCACAGTTCGTCTGATCTCCACCTTGGTCCCCTGTCCAAAGGTG15400.10003065874735634No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)