FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472204
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT58240.3955973492803987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54960.3733178282357608No Hit
GGCTATTAACAGCGCCTATTTAACTTGGTCCCAGCATAAACCTGGCCAGGCTCCAAGGCTCCTCATCTATGC50630.3439061434420773No Hit
CTCTTGTAGGGCCAGTCAGGCTATTAACAGCGCCTATTTAACTTGGTCCC48040.3263134728610981No Hit
ATATTACTGTCAGCAGTTTGGTGACTCATTGTGGACTTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTG47870.32515874158744307No Hit
CTCCAGGCACCGTGTCTTTGTCTCCAGGGGCAAGAGTCACCCTCTCTTGTAGGGCCAGTCAGGCTATTAACAGCG45590.3096717574466582No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTACTACTCTGGCTCCCAGAAACCA43180.29330174350837246No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT39850.27068259561854197No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37930.2576409247631442No Hit
GTTAATAGCCTGACTGGCCCTACAAGAGAGGGTGACTCTTGCCCCTGGAGACAAAGACACGGTGCCTGGAGAC37370.25383710409698657No Hit
GGTTTATGCTGGGACCAAGTTAAATAGGCGCTGTTAATAGCCTGACTGGCCCTACAAGAGAGGGTGACTCTTGCC35860.24358037337216853No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGTGGAAGCAGCATAGATGAGGAGCCT35180.2389614482775485No Hit
GCATAAACCTGGCCAGGCTCCAAGGCTCCTCATCTATGCTGCTTCCACCAGGGCCACTGGCGTCCCAGACAGGT33900.23026700104061665No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33800.22958774735023138No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA33780.22945189661215426No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33180.22537637446984252No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGACTGGAGCCTGAAGATTTTGCAATATATTACTGTCAGCAGTT32860.22320276266060954No Hit
CAGTAATATATTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA32220.2188555390421436No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31660.21505171837598597No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC31650.2149837930069474No Hit
CATCTATGCTGCTTCCACCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA30690.20846295757924854No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29650.20139871919924143No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG28000.19019103330788395No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27770.18862874981999778No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT26450.17966260110691182No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAAAGTC25830.17545122822652295No Hit
GCATAGATGAGGAGCCTTGGAGCCTGGCCAGGTTTATGCTGGGACCAAGTTAAATAGGCGCTGTTAATAGCCTGA25320.17198703440555793No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTACTACTCTGGCTCCCAGAAAC25090.17042475091767173No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT24690.16770773615613052No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC24240.1646510945493967No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT24060.16342843790670314No Hit
CTACTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTC24030.16322466179958756No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGTGGAAGCAGCATAGA23700.16098312462131606No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22930.15575287120534925No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22660.15391888624130895No Hit
GCCTTGGAGCCTGGCCAGGTTTATGCTGGGACCAAGTTAAATAGGCGCTG22130.15031884168226686No Hit
CTACAAGAGAGGGTGACTCTTGCCCCTGGAGACAAAGACACGGTGCCTGG22010.1495037372538045No Hit
GTCTCCAGGCACCGTGTCTTTGTCTCCAGGGGCAAGAGTCACCCTCTCTTGTAGGGCCAGTCAGGCTATTAACAG21410.1454282151114927No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21350.1450206628972615No Hit
GACTTCACTCTCACCATCAGCGGACTGGAGCCTGAAGATTTTGCAATATATTACTGTCAGCAGTTTGGTGACTCA21330.14488481215918447No Hit
GTGCCTGGAGACTGCGTCAACACAATTTCTCCGGTGGTTTCTGGGAGCCA21160.14373008088552944No Hit
ATATATTACTGTCAGCAGTTTGGTGACTCATTGTGGACTTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA20950.14230364813572033No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC20460.13897530505283237No Hit
GTAATATATTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20300.13788849914821585No Hit
CTTGTAGGGCCAGTCAGGCTATTAACAGCGCCTATTTAACTTGGTCCCAGCATAAACCTGGCCAGGCTCCAAGG19480.13231861888705643No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18630.12654496251878136No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18630.12654496251878136No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18570.12613741030455017No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18350.12464305218570253No Hit
CTCCAAGGCTCCTCATCTATGCTGCTTCCACCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTC18190.12355624628108605No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTACTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTCTC17930.12179018668608427No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17640.11982035098396689No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT17150.11649200790107893No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA16650.11309573944915242No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16320.11085420227088094No Hit
GTCCACAATGAGTCACCAAACTGCTGACAGTAATATATTGCAAAATCTTC16250.11037872468761122No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA16190.10997117247338005No Hit
ATTGTGGACTTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGTGG16160.10976739636626447No Hit
GTCTGGGACGCCAGTGGCCCTGGTGGAAGCAGCATAGATGAGGAGCCTTGGAGCCTGGCCAGGTTTATGCTGGGA15730.10684660549760767No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT15520.10542017274779855No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAGCGGACTGGAGCCTGAAGAT15460.10501262053356736No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15340.104197516105105No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15110.10263523261721881No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14780.10039369543894733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT200.00769123651.17593439
AGCTGCG10650.049.77419370
TGTTAGA16400.048.80120570
TCGTATG2950.046.3488740
GGTATCA13700.045.5203361
TATCTCG2850.042.85844436
AATGGTA4900.042.42458370
CGTATGC3250.042.14057541
GCGTAGA33200.040.0733470
GTTATTA1307.814162E-539.9770170
GTGGTAT3550.039.6674271
CGAGACG3900.039.5638223
ATCTCGT3150.038.85550737
ATGCCGT3650.038.70760744
CACGAGA3950.037.35192521
GCCGTCT3800.037.3269746
CAGTTCG24100.037.09070670
GACGGAC4000.036.9427726
GTATCAA34700.036.9102481
ACGAGAC4100.036.8094922
Produced by FastQC (version 0.11.5)