FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1472204
Sequences flagged as poor quality0
Sequence length18-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56310.38248775305596233No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA51520.3499515012865065No Hit
CTCCAGGCACCGTGTCTTTGTCTCCAGGGGCAAGAGTCACCCTCTCTTGTAGGGCCAGTCAGGCTATTAACAGC48030.32624554749205953No Hit
GGCTATTAACAGCGCCTATTTAACTTGGTCCCAGCATAAACCTGGCCAGGCTCCAAGGCTCCTCATCTATGCT46190.31374727958897003No Hit
CTCTTGTAGGGCCAGTCAGGCTATTAACAGCGCCTATTTAACTTGGTCCCAGCATAAACCTGGCCAGGCTCCAAG45980.31232084683916084No Hit
ATATTACTGTCAGCAGTTTGGTGACTCATTGTGGACTTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAACTGT45040.30593586214953905No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41720.2833846396287471No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTACTACTCTGGCTCCCAGAAACCA40480.2749618938679694No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT36510.24799552235967298No Hit
GGTTTATGCTGGGACCAAGTTAAATAGGCGCTGTTAATAGCCTGACTGGCCCTACAAGAGAGGGTGACTCTTGCC36280.24643323887178678No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGTGGAAGCAGCATAGATGAGGAGCCTT34750.23604065740889169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34480.2342066724448514No Hit
GTTAATAGCCTGACTGGCCCTACAAGAGAGGGTGACTCTTGCCCCTGGAGACAAAGACACGGTGCCTGGAGACT33340.22646318037445895No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC33310.2262594042673434No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA32510.22082537474426098No Hit
CAGTAATATATTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA31780.2158668228044483No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCGGACTGGAGCCTGAAGATT31420.2134215095190612No Hit
GCATAAACCTGGCCAGGCTCCAAGGCTCCTCATCTATGCTGCTTCCACCAGGGCCACTGGCGTCCCAGACAGGTT30780.2090742859005953No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT30560.2075799277817476No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC30250.20547424134155323No Hit
CATCTATGCTGCTTCCACCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA28470.19338352565269487No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27150.1844173769396089No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT26390.17925504889268062No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25920.17606255654786973No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC25520.17334554178632852No Hit
GCATAGATGAGGAGCCTTGGAGCCTGGCCAGGTTTATGCTGGGACCAAGTTAAATAGGCGCTGTTAATAGCCT24810.16852284058459288No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCCCTTGGCCGAAAGTC24580.1669605570967067No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA24000.16302088569247197No Hit
CTACTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTCTCCAGGCACCGTGTCTTTGTCTCCA23470.15942084113342989No Hit
GACTTCACTCTCACCATCAGCGGACTGGAGCCTGAAGATTTTGCAATATA23470.15942084113342989No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTACTACTCTGGCTCCCAGAAAC23170.15738308006227397No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23040.1565000502647731No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGGTGGAAGCAGCATA22900.15554909509823367No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA22820.15500569214592544No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22660.15391888624130895No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT22150.1504546924203439No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA22130.15031884168226686No Hit
GCCTTGGAGCCTGGCCAGGTTTATGCTGGGACCAAGTTAAATAGGCGCTGTTAATAGCCTGACTGGCCCTACAA21500.14603954343283945No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT21230.14420555846879915No Hit
GTCTCCAGGCACCGTGTCTTTGTCTCCAGGGGCAAGAGTCACCCTCTCTTGTAGGGCCAGTCAGGCTATTAACA21170.14379800625456798No Hit
ATATATTACTGTCAGCAGTTTGGTGACTCATTGTGGACTTTCGGCCAAGGGACCAGGGTGGAAATCAAACGAA20900.14196402129052768No Hit
GTAATATATTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA20650.14026588706456444No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT19940.1354431858628288No Hit
CTACAAGAGAGGGTGACTCTTGCCCCTGGAGACAAAGACACGGTGCCTGGAGACTGCGTCAACACAATTTCTCCG19830.13469600680340496No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19330.13129973835147846No Hit
GTGCCTGGAGACTGCGTCAACACAATTTCTCCGGTGGTTTCTGGGAGCCAGAGTAGTAGGAGGAAGAGAAGCTGC19270.1308921861372473No Hit
CTTGTAGGGCCAGTCAGGCTATTAACAGCGCCTATTTAACTTGGTCCCAGCATAAACCTGGCCAGGCTCCAAG18740.1272921415782052No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCCTGGTCC18620.12647703714974284No Hit
CTCCAAGGCTCCTCATCTATGCTGCTTCCACCAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTG17980.12212981353127693No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTACTACTCTGGCTCCCAGAAACCACCGGAGAAATTGTGTTGACGCAGTCTC17310.1175788138056954No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17040.1157448288416551No Hit
GTCTGGGACGCCAGTGGCCCTGGTGGAAGCAGCATAGATGAGGAGCCTTGGAGCCTGGCCAGGTTTATGCTGG16450.11173723206838182No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16190.10997117247338005No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT15990.10861266509260945No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15960.10840888898549386No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15440.1048767697954903No Hit
GTCCACAATGAGTCACCAAACTGCTGACAGTAATATATTGCAAAATCTTCAGGCTCCAGTCCGCTGATGGTGAGA15400.10460506831933619No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG15270.10372203852183529No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC15230.10345033704568117No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15030.10209182966491057No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14880.10107294912933262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA5450.063.72528570
TTACGCA250.007689911451.177568
GTTAACA850.00774706651.0739470
ATGGCAA4000.050.64832370
GAGCAAA33200.047.07417370
GGTATCA15150.039.985691
GCAGTTT28800.038.68969370
GTGGTAT4450.038.357371
TGGTATC4600.037.071292
ACTCAGT9600.036.17737270
GTATCAA35400.035.548661
GTAGTTT2051.6257321E-435.29500270
ATACGTA1600.033.4368511
AAGGACA34250.031.26570170
GCGGGAA32600.031.07259270
ACGTACC1750.030.57291213
GAGCCAT11850.030.5294370
CGTTATA553.8193748E-430.52363427
AAGCGGT9550.030.30565570
TAGGCAA6301.8189894E-1229.8606970
Produced by FastQC (version 0.11.5)