FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264639
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAG36330.2872756573219709No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG36200.2862476959828062No Hit
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTAGACGTGTCTACTGA34900.27596808259115846No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTACTACCTGCATATGAGCAGCAGTAATAA31430.24852942223037564No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC30790.24346868948371828No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC30200.2388033264828935No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG29700.2348496290245675No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC29190.23081685761707493No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG28700.22694223410791536No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGG28650.22654686436208277No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT27110.21436947619043853No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC26530.2097831871387803No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTACTAC25340.20037338718796432No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC24400.19294043596631133No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCGCCAGCACCCAGGGAAGGGCCTGGAG23370.18479581920215968No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTACTACCTGCATATGAGCAG23130.18289804442216318No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG20500.16210159579136812No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACGCAGGCAAAGC20430.16154807814720248No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC20320.16067826470637075No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG20290.1604410428588712No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18890.14937068997555825No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18630.1473147672972287No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTA18300.14470532697473348No Hit
GTGTATTACTGTGCGAGAATGCCTCCGGGGGACCTTGATGCTTTTGATAT17880.1413842211097396No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACGCAGGCAAAGCCCCCAAA17650.13956552027890962No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG17380.13743052365141356No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCA17040.13474200937975186No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC16940.13395126988808664No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT16700.1320534951080901No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGT16550.13086738587059232No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG16220.12825794554809714No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC16170.1278625758022645No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15820.12509498758143628No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC15280.12082499432644414No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCC14430.11410370864728986No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTAGACGTGTCTA14340.11339204310479116No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14190.11220593386729336No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC14170.11204778596896031No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAG14130.11173149017229422No Hit
GGTACCAACAGCACGCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGC13550.10714520112063601No Hit
GTACCAACAGCACGCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGCGGCCCTCAGGGGTTTCTAA13040.10311242971314342No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGA13030.10303335576397693No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12780.10105650703481389No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT12770.10097743308564737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT354.434696E-968.4497239
AGGGGGG3950.058.92473670
TCGTATG2350.051.00986540
CGTATGC2550.048.39668741
GGTATCA9700.042.2859151
TCTCGTA2250.041.025638
CTTATAC3550.040.1054151
ACGAGAC3350.038.44457222
GTATCAA23550.038.0010071
ATCTCGT2450.037.64053737
CCCACGA3350.037.42991319
GACCGGA2005.585116E-737.0288470
GTATGCC3400.036.33530842
CGAGACA3650.036.21758723
TATACAC3850.036.09273
ATGCCGT3350.035.9294944
ATCTCCG3600.035.76915410
CCGTCTT3350.035.0266147
CACGAGA3600.034.8319921
AATCTCG2700.034.1321836
Produced by FastQC (version 0.11.5)