FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264639
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTAGACGTGTCTACT32970.2607068104020199No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA32240.25493441211286383No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31760.2511388625528708No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC31040.2454455382128813No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTACTACCTGCATATGAGCAGCAGTAATAAT28870.22828649124374623No Hit
GATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGT27720.2191929870895963No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT27560.21792780390293198No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG27410.2167416946654342No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG26900.21270892325794158No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTT25420.20100597878129647No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC25310.20013616534046474No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25160.19895005610296693No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT24650.19491728469547437No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTACTACCTGCATATGAGCA23130.18289804442216318No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTACTAC22870.18084212174383363No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22780.18013045620133494No Hit
GTCTCTGGTGGCTCCATCAGCAGTGGTGGTTACTACTGGAGCTGGATCCG20520.16225974368970117No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC19880.15719901094304384No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19660.15545938406138035No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCACGCAGGCAAAGC19470.15395697902721647No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18900.14944976392472475No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA18700.14786828494139434No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGA17380.13743052365141356No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGCTA17170.1357699707189166No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG16890.13355590014225402No Hit
GTTATAACCTTGTCTCCTGGTACCAACAGCACGCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCAGTAAGC16820.13300238249808838No Hit
GTGTATTACTGTGCGAGAATGCCTCCGGGGGACCTTGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACC16740.13236979090475623No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGGAGTTATAACCTTGTCTCCTGGTACCAACAGCA16280.12873238924309627No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT16250.1284951673955967No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT16200.1280997976497641No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG16040.12683461446309977No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACACCTGTGGTTCTTCCTCCTGCTGGTGGCAGCTCCCAGATGGGTC15790.12485776573393673No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGT15390.12169480776727588No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG15350.1213785119706098No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT15270.12074592037727763No Hit
GGCCTGGGCTCTGCTGCTCCTCACTCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC14990.11853184980061503No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG14460.11434093049478941No Hit
CAGTAATACACGGCCGTGTCCGCGGCAGTCACAGAGCTCAGCTTCAGGGAGAACTGATTCTTAGACGTGTCTA14290.11299667335895856No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT14030.11094075068062903No Hit
CTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTG13670.10809408851063426No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT13370.10572187003563864No Hit
GTACCAACAGCACGCAGGCAAAGCCCCCAAACTCATGATTTATGAGGGCA13140.10390316920480866No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC12890.10192632047564562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA2650.062.74429370
CGATTGT700.004266750559.3829970
GTAGTTT3001.6370905E-1146.1867770
CGGCAAA2507.734343E-944.33930270
GTATCAA27500.040.3859671
GGTATCA10500.040.3097461
AAGCGGT6200.040.2271970
ATAATCA22250.036.74183770
CGAAACC901.0064121E-535.9800169
CTGGAGT16300.035.70265670
CAACGCA32200.034.574395
ATCAACG32050.034.5264053
AACGCAG32800.034.251436
TCAACGC32250.034.2046554
TATCAAC32650.033.7896162
CCCGTCA21400.033.02138570
ACGCAGA34100.032.6479577
AGGCGAA1700.003422025432.6024370
ACTCAGT10350.032.1299370
AGAGTAC34500.032.0745311
Produced by FastQC (version 0.11.5)