FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931814
Sequences flagged as poor quality0
Sequence length19-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG49180.5277877344620278No Hit
GCCTTGGGCTGACCGAGGACGGTCACCTTCGTCCCTCCGCCGAAAACCACATGACTGCTCAATGTATATGCGTTA34650.3718553273507374No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29560.3172306919621298No Hit
CATATACATTGAGCAGTCATGTGGTTTTCGGCGGAGGGACGAAGGTGACCGTCCTCGGTCAGCCCAAGGCTGCCC27610.29630376877788916No Hit
ATGTATATGCGTTACAATAATAGTCAGCCTCGTCCTGAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTATTGC26950.2892208101616846No Hit
GTCATGTGGTTTTCGGCGGAGGGACGAAGGTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGT26870.28836226972335677No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG26220.28138662866194325No Hit
CCTATGAACATGCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT25150.26990365029930863No Hit
GTCGATTACCATCTCCTGCACTGGAACCAACACTGACGTCGGTGCTCACAACCTTGTCACCTGGTACCAACAGT22530.24178645094407253No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTCGTCCCTC22410.2404986402865808No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19960.21420583936279128No Hit
GGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCGATTAC19250.2065862929726319No Hit
GTACCAACAGTTCCCTGGGAAAGCCCCCAAGATGATACTTTTTGAAGTCAGTCGTCGGCCCTCAGGGGTCTCTAG19170.20572775253430406No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18220.1955325848291612No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17750.19048865975398524No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17630.1892008490964935No Hit
CTATTATTGTAACGCATATACATTGAGCAGTCATGTGGTTTTCGGCGGAG17610.18898621398691157No Hit
CCACATGACTGCTCAATGTATATGCGTTACAATAATAGTCAGCCTCGTCC17200.18458619424048145No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17130.18383497135694463No Hit
CTCCTGCACTGGAACCAACACTGACGTCGGTGCTCACAACCTTGTCACCTGGTACCAACAGTTCCCTGGGAAAGC16930.18168862026112506No Hit
TCTCTGGCCTCCAGGCTCAGGACGAGGCTGACTATTATTGTAACGCATAT16700.1792203165009326No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16430.1763227425215762No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA16270.17460566164492056No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCGATTACCATCTCCTGCACTGGAACCAACACTGACG15580.16720075036434312No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15530.16666416259038822No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15330.16451781149456865No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15050.16151291996042128No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14720.15797144065231902No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14710.15786412309752806No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG14600.1566836299948273No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14560.1562543597756634No Hit
CTCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGCCCTGAGTGGATGG14390.15442996134421677No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC14370.1542153262346348No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGC14010.1503518942621596No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14000.15024457670736863No Hit
CAATAATAGTCAGCCTCGTCCTGAGCCTGGAGGCCAGAGATGGTCAGGGA13980.15002994159778668No Hit
CGCATATACATTGAGCAGTCATGTGGTTTTCGGCGGAGGGACGAAGGTGACCGTCCTCGGTCAGCCCAAGGCTGC13740.14745432028280323No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13520.14509333407740171No Hit
GGACGAGGCTGACTATTATTGTAACGCATATACATTGAGCAGTCATGTGGTTTTCGGCGGAGGGACGAAGGTGAC13490.14477138141302878No Hit
CAATAATAGGTGGCCGTGTCCTCAGACGTCAGGCTGGTCAACTCCATGTA12710.13640061213933255No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT12710.13640061213933255No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12550.13468353126267688No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCGATTACCATCTCCTGCACTGGAACCAACA12350.13253718016685734No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC12250.13146400461894756No Hit
GTATATGCGTTACAATAATAGTCAGCCTCGTCCTGAGCCTGGAGGCCAGA12230.1312493695093656No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT12180.1307127817354107No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG12130.13017619396145583No Hit
ATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCGATTACCATCTCC12090.1297469237422919No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATGC11960.12835179553000922No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11860.12727861998209944No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA11840.1270639848725175No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11810.12674203220814456No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11730.12588349176981672No Hit
ATAATAGGTGGCCGTGTCCTCAGACGTCAGGCTGGTCAACTCCATGTAGGCTGTGTCTGTGGACGTGTCCTCGG11720.12577617421502574No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTG11710.12566885666023478No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11670.12523958644107086No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCT11280.12105420180422272No Hit
GCCTCGTCCTGAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTATTGCC11270.12094688424943176No Hit
CTCCGGGACAGTCGATTACCATCTCCTGCACTGGAACCAACACTGACGTC11240.12062493158505883No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11150.11965907359194003No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCG11000.11804931027007537No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAAC10970.11772735760570242No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTCGTCCCTCCGCCGAAAACCACA10940.11740540494132949No Hit
AGATGATACTTTTTGAAGTCAGTCGTCGGCCCTCAGGGGTCTCTAGTCGCTTCTCTGGCTCCAAGTCCGGCAATA10890.1168688171673746No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTCGTCCCTCCGCCGAAAACCACATGAC10850.1164395469482107No Hit
GCTCACAACCTTGTCACCTGGTACCAACAGTTCCCTGGGAAAGCCCCCAAGATGATACTTTTTGAAGTCAGTCG10840.11633222939341971No Hit
GTGCATGGAGAGTTGACTGAGGGTGTCTCCGGAAACCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC10830.11622491183862875No Hit
CCATATACACACAGAAATTTCAGGGCAGAATCTCCATGACCGAGGACACGTCCACAGACACAGCCTACATGGAGT10830.11622491183862875No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT10530.11300538519489942No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10510.11279075008531746No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG10420.11182489209219865No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG10400.1116102569826167No Hit
GTATCATCTTGGGGGCTTTCCCAGGGAACTGTTGGTACCAGGTGACAAGGTTGTGAGCACCGACGTCAGTGTTGG10300.11053708143470692No Hit
CTGTTGGTACCAGGTGACAAGGTTGTGAGCACCGACGTCAGTGTTGGTTCCAGTGCAGGAGATGGTAATCGA10260.11010781121554303No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATGCTGTAGGGGC10250.11000049366075204No Hit
GACCGAGGACGGTCACCTTCGTCCCTCCGCCGAAAACCACATGACTGCTCAATGTATATGCGTTACAATAATAG10250.11000049366075204No Hit
GGTACCAACAGTTCCCTGGGAAAGCCCCCAAGATGATACTTTTTGAAGTC10000.1073175547909776No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9870.1059224265786949No Hit
GGTCCAACTTGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAG9710.10420534570203925No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCT9650.10356144037329339No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG9610.10313217015412947No Hit
GTCATGGAGATTCTGCCCTGAAATTTCTGTGTGTATATGGTCTCCACTTTTTCAGGATCAAAACCTCCCATCCA9570.10270289993496556No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCC9400.10087850150351893No Hit
GTTGAGAACCTATGAACATGCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG9390.10077118394872797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT150.00242113568.5226739
GGTACGG150.002489284468.044654
AGGGGGG3000.066.3588170
CAAGCCG550.00496539957.15112370
TTGAGTA200.00736809251.7330747
GCTATAA200.00747164251.55079343
CGACTTT200.00756333551.39200239
AGGTACG200.0077755951.0334853
GGTATCA6050.050.078931
CGTGAAA306.761398E-445.36066410
CGTATGC2700.044.50182341
TCTCGCG8450.043.39878570
GTATCAA18200.043.0206381
GAGCGAT2650.041.21404632
TCGTATG2600.040.89867440
CCGTCTT2700.040.8755147
ATGCCGT2800.040.52836244
TGAGCGA2950.040.4849631
GGCGGCG8800.040.48204470
GTGGTAT1100.040.231951
Produced by FastQC (version 0.11.5)