FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931814
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT47280.5073973990517421No Hit
GCCTTGGGCTGACCGAGGACGGTCACCTTCGTCCCTCCGCCGAAAACCACATGACTGCTCAATGTATATGCGTTA33910.363913828296205No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32670.35060645150212383No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG29970.32163071170855984No Hit
GTCATGTGGTTTTCGGCGGAGGGACGAAGGTGACCGTCCTCGGTCAGCCC27480.29490864056560645No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25820.2770939264703042No Hit
ATGTATATGCGTTACAATAATAGTCAGCCTCGTCCTGAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTATTGC25520.27387439982657485No Hit
CATATACATTGAGCAGTCATGTGGTTTTCGGCGGAGGGACGAAGGTGACCGTCCTCGGTCAGCCCAAGGCTGCCC24670.2647524076693417No Hit
CCTATGAACATGCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT24010.2576694490531372No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTCGTCCCTC21000.22536686506105297No Hit
GTCGATTACCATCTCCTGCACTGGAACCAACACTGACGTCGGTGCTCACAACCTTGTCACCTGGTACCAACAGTT20860.22386441929397927No Hit
GGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCGATTAC19520.20948386695198826No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19310.20723019830137776No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA19180.20583507008909505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA19030.20422530676723039No Hit
GTACCAACAGTTCCCTGGGAAAGCCCCCAAGATGATACTTTTTGAAGTCA18220.1955325848291612No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17490.18769840332941984No Hit
CCACATGACTGCTCAATGTATATGCGTTACAATAATAGTCAGCCTCGTCCTGAGCCTGGAGGCCAGAGATGGTCA17450.18726913311025592No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCA17220.18480082935006342No Hit
CTATTATTGTAACGCATATACATTGAGCAGTCATGTGGTTTTCGGCGGAGGGACGAAGGTGACCGTCCTCGGTCA16900.18136666759675213No Hit
CTCCTGCACTGGAACCAACACTGACGTCGGTGCTCACAACCTTGTCACCTGGTACCAACAGTTCCCTGGGAAAGC16490.17696664785032207No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15830.16988368923411754No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15780.16934710146016266No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG15680.16827392591225288No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA15350.1647324466041506No Hit
TCTCTGGCCTCCAGGCTCAGGACGAGGCTGACTATTATTGTAACGCATATACATTGAGCAGTCATGTGGTTTTCG14590.15657631244003634No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14410.15464459645379872No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCGATTACCATCTCCTGCACTGGAACCAACACTGACGT14270.15314215068672501No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG14110.1514250698100694No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT13790.1479909080567581No Hit
CTCCATGCACTGGGTGCGACAGGCTCCTGGAAAAGGCCCTGAGTGGATGGGAGGTTTTGATCCTGAAAAAGTGGA13790.1479909080567581No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT13750.1475616378375942No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13690.14691773250884835No Hit
GGACGAGGCTGACTATTATTGTAACGCATATACATTGAGCAGTCATGTGGTTTTCGGCGGAGGGACGAAGGTGAC13630.14627382718010248No Hit
CGCATATACATTGAGCAGTCATGTGGTTTTCGGCGGAGGGACGAAGGTGACCGTCCTCGGTCAGCCCAAGGCTGC13230.14198112498846335No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG13160.14122990210492653No Hit
CAATAATAGTCAGCCTCGTCCTGAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTATTGCCGGACTTGGAGCCA13150.14112258455013554No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGC13070.14026404411180773No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCCCAGTC12830.13768842279682428No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG12690.13618597702975058No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG12650.13575670681058666No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG12500.134146943488722No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG12390.13296645038602123No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCGATTACCATCTCCTGCACTGGAACCAACA12390.13296645038602123No Hit
CAATAATAGGTGGCCGTGTCCTCAGACGTCAGGCTGGTCAACTCCATGTAGGCTGTGTCTGTGGACGTGTCCTCG11810.12674203220814456No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC11760.12620544443418966No Hit
ATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCGATTACCATCTCCT11730.12588349176981672No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT11640.12491763377669791No Hit
GTGCATGGAGAGTTGACTGAGGGTGTCTCCGGAAACCTTGCAGGAGACCTTCACTGAGGCCCCAGGCTTCTTCAC11550.12395177578357912No Hit
CTCCGGGACAGTCGATTACCATCTCCTGCACTGGAACCAACACTGACGTC11470.12309323534525131No Hit
GCCTCGTCCTGAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTATTGCCGGACTTGGAGCCAGAGAAGCGACTA11390.12223469490692349No Hit
CCATATACACACAGAAATTTCAGGGCAGAATCTCCATGACCGAGGACACG11240.12062493158505883No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11160.11976639114673099No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11160.11976639114673099No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC11020.11826394537965731No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTCGTCCCTCCGCCGAAAACCACATGACT10930.11729808738653852No Hit
GTATATGCGTTACAATAATAGTCAGCCTCGTCCTGAGCCTGGAGGCCAGAGATGGTCAGGGAGGCCGTATTGCCG10860.11654686450300167No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATGCTGTAGGGGCCACTGTCTTCTCCACG10730.11515173629071897No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT10530.11300538519489942No Hit
GTATCATCTTGGGGGCTTTCCCAGGGAACTGTTGGTACCAGGTGACAAGG10460.11225416231136257No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCGGGACAGTCG10440.11203952720178062No Hit
AGATGATACTTTTTGAAGTCAGTCGTCGGCCCTCAGGGGTCTCTAGTCGCTTCTCTGGCTCCAAGTCCGGCAATA10380.11139562187303476No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA10370.11128830431824377No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT10330.11085903409907985No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCACCTTCGTCCCTCCGCCGAAAACCACAT10250.11000049366075204No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG10230.1097858585511701No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTCCTCCGACGGCGGC10220.10967854099637911No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCT10060.10796146011972346No Hit
ATAATAGGTGGCCGTGTCCTCAGACGTCAGGCTGGTCAACTCCATGTAGGCTGTGTCTGTGGACGTGTCCTCGGT9930.10656633190744076No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC9910.10635169679785879No Hit
GTGTATATGGTCTCCACTTTTTCAGGATCAAAACCTCCCATCCACTCAGGGCCTTTTCCAGGAGCCTGTCGCACC9890.10613706168827684No Hit
GGTCCAACTTGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGTTTCCG9810.10527852124994903No Hit
CTGTTGGTACCAGGTGACAAGGTTGTGAGCACCGACGTCAGTGTTGGTTCCAGTGCAGGAGATGGTAATC9730.1044199808116212No Hit
GGTACCAACAGTTCCCTGGGAAAGCCCCCAAGATGATACTTTTTGAAGTCAGTCGTCGGCCCTCAGGGGTCTCTA9720.10431266325683024No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCT9720.10431266325683024No Hit
GCTCACAACCTTGTCACCTGGTACCAACAGTTCCCTGGGAAAGCCCCCAAGATGATACTTTTTGAAGTCAGTCGT9640.1034541228185024No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9580.10281021748975654No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAACGTGGTCGTCGCATGCCTGGTCCAGG9550.1024882648253836No Hit
GTCATGGAGATTCTGCCCTGAAATTTCTGTGTGTATATGGTCTCCACTTTTTCAGGATCAAAACCTCCCATCCA9540.10238094727059263No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9510.1020589946062197No Hit
GACCGAGGACGGTCACCTTCGTCCCTCCGCCGAAAACCACATGACTGCTCAATGTATATGCGTTACAATAATAGT9480.10173704194184677No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC9460.1015224068322648No Hit
GTTGAGAACCTATGAACATGCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA9430.10120045416789186No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATGCTGTAGGGGC9350.10034191372956405No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG9340.10023459617477307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCAGT550.001229917581.25481470
AATGGTA2450.079.0437970
TAAGTCA1351.1623904E-555.1730270
TAGCGCG200.00784101850.9254264
CAGTCAA11500.050.51929570
TAGGCAA3007.2759576E-1249.6557270
GGTAGAA20550.046.39366570
TGACAGT16200.045.97751670
GGTATCA6350.045.5038641
GTATCAA19250.043.265171
AGTGGAA11800.042.92273770
CAGCCAT10900.041.00013770
CTCTGTT22750.039.28804470
TATCAAC21650.037.80442
TCTTGTT3203.5957783E-837.2417970
ATCAACG22500.036.3625763
TCAACGC22350.036.3047754
TGGAGTT11900.036.30291770
GCACCGT9850.036.29656670
CAACGCA22550.035.9827845
Produced by FastQC (version 0.11.5)