FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046434
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCCGCAGCGGTCACAGAGCTCAGTTTCAGGGAGAACTGGTTCTTCGACGTGTCTACTGA38290.3659093645657538No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33010.31545228843864015No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT28060.2681487795694712No Hit
GTCTCTGGTGGCTCCGTCAGTACGCACTACTGGACTTGGATCCGGCAGGCCCCCGGGAAGGGACTGGAGTGGAT27950.26709759048348963No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26110.24951406395434397No Hit
ATACAATACAGTGAGACCATCAACTACAACCCCTCCCTCAGGAGTCGAGT24820.23718648285510602No Hit
GTATAATTACGTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC24730.23632641905748475No Hit
TAATTATACTGTAACTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGT24400.23317285179954017No Hit
GTATTGTATATATCCAATCCACTCCAGTCCCTTCCCGGGGGCCTGCCGGA24160.23087934833921683No Hit
CAGTAATACATGGCCGTGTCCGCAGCGGTCACAGAGCTCAGTTTCAGGGAGAACTGGTTCTTCGACGTGTCTA22030.21052450512884713No Hit
ATGTATTACTGTGCGAGAGATAATTTAAATAGTGGTTCCGTCTTTGACTA22020.21042894248466695No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT21760.20794431373598335No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC21120.20182830450845443No Hit
CTACTGGACTTGGATCCGGCAGGCCCCCGGGAAGGGACTGGAGTGGATTGGATATATACAATACAGTGAGACCA20190.19294097859970147No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG19560.1869205320163527No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG19130.1828113383166067No Hit
GTATTACTGTGCGAGAGATAATTTAAATAGTGGTTCCGTCTTTGACTACTGGGGCCAGGGAATCCTGGTCACCG18920.18080452278882378No Hit
GTATATATCCAATCCACTCCAGTCCCTTCCCGGGGGCCTGCCGGATCCAAGTCCAGTAGTGCGTACTGACGGAGC18880.18042227221210322No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAAGATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18530.17707757966579832No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18390.17573970264727637No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18080.17277726067769203No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17810.17019706928482828No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17720.16933700548720704No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGCGGATCTGGGACAGAATTCACT17310.1654189370758213No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17050.16293430832713768No Hit
GATCTATGCTGCATCCAGTTTGCACGGTGGGGTCCCATCAAGGTTCAGTGGCAGCGGATCTGGGACAGAATTCA16970.16216980717369658No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG16290.1556715473694471No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC15640.14945997549773804No Hit
GAGTTACAGTATAATTACGTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTGTGGCTGCACCATCTGTCT15640.14945997549773804No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA15370.1468797841048743No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15080.14410846742365022No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14900.1423883398284077No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14480.13837470877284186No Hit
GGATATATACAATACAGTGAGACCATCAACTACAACCCCTCCCTCAGGAGTCGAGTCACCATATCAGTAGACACG14310.1367501438217795No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT14230.13598564266833837No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13630.13025188401753002No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC13560.12958294550826904No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13410.12814950584556695No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13380.12786281791302653No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC13170.1258560023852436No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC12890.1231802483481997No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12830.12260687248311887No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG12690.12126899546459691No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12450.11897549200427357No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTCGCTGATACCAATTTAAATATCTATTAATGTTCTCACT12150.11610861267886939No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC12040.11505742359288786No Hit
GTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAACGTA12020.11486629830452758No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11860.11333729599764533No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11770.11247723220002408No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11750.1122861069116638No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG11700.1118082936907631No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG11520.11008816609552059No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC11500.10989704080716031No Hit
CCTTGGGGCTGGTCGGGGATGCGGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGTAGTCAAAGACGGAACCA11230.10731684941429655No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10940.1045455327330725No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG10890.10406771951217181No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCC10810.1033032183587307No Hit
ACCAGAGACAGTGCACGTGAGGGACAGGGTCTCCGAAGGCCTCACCAGTCCTGGGCCCGACTCCAACAGCTGCAC10690.10215646662856902No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10640.10167865340766832No Hit
GGACTGGTGAGGCCTTCGGAGACCCTGTCCCTCACGTGCACTGTCTCTGGTGGCTCCGTCAGTACGCACTAC10560.10091415225422722No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCGGAGGAGACGGTGACCAGGATTCCC10560.10091415225422722No Hit
GGACTGGAGTGGATTGGATATATACAATACAGTGAGACCATCAACTACAA10480.1001496511007861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTTCG550.00586185454.8055770
TGAGTAA1501.4460966E-953.58766670
TCGTATG1750.052.86629540
CGTATGC1800.051.46501541
CTAGCGA200.0075602251.39778540
ATCTCGT1750.050.72912637
TGTTAGA8000.048.9824870
GAGTGAA12350.048.8146870
ACTGACG11250.046.44264670
GTCACCG10900.045.16850370
ACGAGAC2150.044.0932722
GCCGTCT2250.042.9507446
ATGCCGT2250.042.8421844
GGGGGTA959.5225696E-442.30605370
GTATGCC2200.042.16288442
CGAGACA2300.041.2195923
CACGAGA2350.040.3387221
TTATACA2450.040.058792
TATACAC2450.040.058793
AATCTCG2300.040.038436
Produced by FastQC (version 0.11.5)