FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1046434
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATGGCCGTGTCCGCAGCGGTCACAGAGCTCAGTTTCAGGGAGAACTGGTTCTTCGACGTGTCTACT34470.3294044344889406No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA31850.304367021713744No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26630.2544833214517112No Hit
GTCTCTGGTGGCTCCGTCAGTACGCACTACTGGACTTGGATCCGGCAGGCCCCCGGGAAGGGACTGGAGTGGATT26230.25066081568450566No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24790.23689979492256558No Hit
ATACAATACAGTGAGACCATCAACTACAACCCCTCCCTCAGGAGTCGAGTCACCATATCAGTAGACACGTCGAA24590.2349885420389628No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC22150.21167125685900878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21960.20985556661958613No Hit
GTATAATTACGTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCC21910.20937775339868545No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT21790.2082310016685238No Hit
GTATTGTATATATCCAATCCACTCCAGTCCCTTCCCGGGGGCCTGCCGGATCCAAGTCCAGTAGTGCGTACTGAC21600.2064153114291011No Hit
CAGTAATACATGGCCGTGTCCGCAGCGGTCACAGAGCTCAGTTTCAGGGAGAACTGGTTCTTCGACGTGTCTA21140.2020194297968147No Hit
TAATTATACTGTAACTCTGTTGACAGAAGTAAGTTGCAAAATCTTCAGGT20930.2000126142690318No Hit
CTACTGGACTTGGATCCGGCAGGCCCCCGGGAAGGGACTGGAGTGGATTGGATATATACAATACAGTGAGACCAT18980.1813778986539046No Hit
ATGTATTACTGTGCGAGAGATAATTTAAATAGTGGTTCCGTCTTTGACTACTGGGGCCAGGGAATCCTGGTCACC18840.18004002163538266No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAAGATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC18550.17726870495415858No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17970.1717260715917105No Hit
GTGGGGTCCCATCAAGGTTCAGTGGCAGCGGATCTGGGACAGAATTCACTCTCACCATCAGCAGTCTGCAACCT17840.1704837572173687No Hit
GTATTACTGTGCGAGAGATAATTTAAATAGTGGTTCCGTCTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGT17780.16991038135228786No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17570.16790356582450494No Hit
GTATATATCCAATCCACTCCAGTCCCTTCCCGGGGGCCTGCCGGATCCAAGTCCAGTAGTGCGTACTGACGGAGC17220.16455887327820007No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG17050.16293430832713768No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG16640.15901623991575198No Hit
GATCTATGCTGCATCCAGTTTGCACGGTGGGGTCCCATCAAGGTTCAGTGGCAGCGGATCTGGGACAGAATTCA16040.15328248126494362No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15410.14726203468159482No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15080.14410846742365022No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15040.14372621684692966No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA15030.1436306542027495No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA14910.14248390247258785No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14850.141910526607507No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC14660.14009483636808437No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14430.13789689555194115No Hit
GAGTTACAGTATAATTACGTTCGGCCCTGGGACCAAAGTGGATTTCAAACGAACTGTGGCTGCACCATCTGTCTT14270.13636789324505894No Hit
GAATTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTA14060.134361077717276No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13940.13321432598711433No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA13840.13225869954531294No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13760.13149419839187182No Hit
GGATATATACAATACAGTGAGACCATCAACTACAACCCCTCCCTCAGGAG13490.12891400699900807No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13100.12518706387598263No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12730.12165124604131747No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTTCGCTGATACCAATTTAAATATCTATTAATGTTCTCACTT12360.11811542820665231No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12210.11668198854395022No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12160.11620417532304952No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG11620.11104379253732198No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11510.10999260345134045No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA11330.10827247585609794No Hit
GTGCAGCCACAGTTCGTTTGAAATCCACTTTGGTCCCAGGGCCGAACGTA11120.10626566032831501No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11070.10578784710741432No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11020.10531003388651362No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10890.10406771951217181No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGCAGAGGCTCAGCGGGAAGACCTTGGG10890.10406771951217181No Hit
GGACTGGTGAGGCCTTCGGAGACCCTGTCCCTCACGTGCACTGTCTCTGGTGGCTCCGTCAGTACGCACTACTG10890.10406771951217181No Hit
GGACTGGAGTGGATTGGATATATACAATACAGTGAGACCATCAACTACAA10740.10263427984946973No Hit
CCTTGGGGCTGGTCGGGGATGCGGAGGAGACGGTGACCAGGATTCCCTGGCCCCAGTAGTCAAAGACGGAACCA10580.1011052775425875No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGGT10550.10081858961004707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAA401.9475483E-6147.5942770
AATGGTA3650.068.7425470
AACCTAT900.00897861249.1980970
GAGTGAA13100.043.9402870
GTTTGGT1806.724359E-540.9984170
GAGCAAA17900.038.75380370
GAGCCAT9700.038.0397670
GTACGAA600.001937525536.6182369
GTTAGGG656.439968E-736.3708651
GCCGTTA656.4738015E-736.34652715
GTATCAA24050.034.9665871
AGTATAA13250.034.5314970
TAGGCAA3559.7292286E-833.2606870
TAATCGT900.006081638432.79872570
TATTCTA1850.00379833531.91227370
TCACCGT9500.031.80004169
CCGTTAA751.9804957E-631.5018316
CGCGACT450.005144386630.00317818
GACACGT14850.029.81702270
TATCAAC28150.029.4912662
Produced by FastQC (version 0.11.5)