FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128367
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACTTTCCCTCCAAAAACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA56660.5021415904577146No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT46970.41626527539355546No Hit
GTGTTAGACTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATATGTC46970.41626527539355546No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC43800.3881715789277779No Hit
GGGTATTAGCAGGTGGTTAACCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAACCTCCTGATCTATGGTGCATC38190.3384537123116858No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34070.3019407692709907No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT32090.2843932869358994No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28190.24983006415465892No Hit
GATCTATGGTGCATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACATATTTCA27760.24601924728390673No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC27370.24256292500578266No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27060.23981559191291485No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG26200.23219395817141056No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG25700.2277627757635592No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGGTGGTTAACCTGGTATCAGCAGAAA25570.22661066833751783No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24430.21650757244761676No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC24360.2158872069105176No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23720.21021529342846787No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23550.20870869140979842No Hit
CCATAGATCAGGAGGTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGT23070.20445475629826113No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG22720.20135292861276516No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21490.19045221988945085No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAAGTTTTTGGAGGGAAAGTGTTAGACTGT21380.18947735975972355No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT21080.18681865031501277No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACA20930.18548929559265734No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20170.1787538983327233No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC19780.17529757605459925No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACACGGAAGATGGAGAC18660.16537172746101225No Hit
GTAATACATGGCGGTGTCCGAGGCCTCCAGGCTGCTCCACTGAAGGTAGG18400.16306751260892954No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18360.16271301801630145No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17650.15642073899715253No Hit
ATTGTCAACAGTCTAACACTTTCCCTCCAAAAACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTG17520.15526863157111118No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC17350.15376202955244173No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17180.15225542753377228No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16720.14817873971854903No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16440.14569727757015227No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGCTGACCCAGTCTCCATCTTCCG16290.14436792284779684No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15650.13869600936574714No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15450.1369235364026066No Hit
ATGTATTACTGTGCGAGACTCAACTATGTTGCGGGGAGTTATTCCCCAAA15400.13648041816182147No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGG15370.13621454721735038No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA14720.13045401008714363No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT14580.12921327901294527No Hit
ACCATATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAAGTCCATCAGCACCGCCTACCTTCAG14550.12894740806847418No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC14310.12682044051270552No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14090.12487072025325094No Hit
GTATTACTGTGCGAGACTCAACTATGTTGCGGGGAGTTATTCCCCAAACTACTATTACGGTATGGACGTCTGGGG13840.12265512904932527No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT13390.11866706488225906No Hit
GTATTAGCAGGTGGTTAACCTGGTATCAGCAGAAACCAGGAAAAGCCCCT13380.11857844123410204No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCAGCTGGATGTCGCATCTGGAACCTGGGAACCAGAG13140.11645147367833338No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA13050.11565386084492013No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATATGTCCCAGATCCA13010.11529936625229203No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12840.11379276423362256No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12840.11379276423362256No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT12800.11343826964099446No Hit
GTATATGGTATGAGAGTCACCAGGGTAGATGATCCCCATCCACTCCAGGC12640.11202029127048205No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12610.11175442032601095No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12580.11148854938153986No Hit
CAGTAATACATGGCGGTGTCCGAGGCCTCCAGGCTGCTCCACTGAAGGTAGGCGGTGCTGATGGACTTGTCGG12450.11033644195549852No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12160.10776635615894474No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12080.10705736697368853No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG12070.1069687433255315No Hit
CATCAAGGTTCAGCGGCAGTGGATCTGGGACATATTTCACTCTCACCATCAGCAGCCTCCAGCCTGAAGATTT12030.10661424873290339No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA11990.10625975414027529No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11870.10519627036239096No Hit
ACCTTGATGGGACCCCGCTTTGCAAACTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTTCCTGGTTTC11820.10475315212160581No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11820.10475315212160581No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11750.10413278658450663No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11700.10368966834372151No Hit
AGTCAGGGTATTAGCAGGTGGTTAACCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAACCTCCTGATCTATGG11620.10298067915846529No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11490.10182857173242393No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11420.10120820619532475No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)