FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685371

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685371
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128367
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACTTTCCCTCCAAAAACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT53470.47387064669562295No Hit
GTGTTAGACTGTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATATGTC49410.43788944554387No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT47030.41679701728249763No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA42840.37966370870470334No Hit
GGGTATTAGCAGGTGGTTAACCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAACCTCCTGATCTATGGTGCATC34270.30371324223413126No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32470.28776098556586643No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT30680.27189735254575864No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC28670.2540839992661962No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCA27830.24663961282100594No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA25330.22448370078174917No Hit
GATCTATGGTGCATCCAGTTTGCAAAGCGGGGTCCCATCAAGGTTCAGCGGCAGTGGATCTGGGACATATTTCA24410.2163303251513027No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC24160.21411473394737707No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT23450.20782245492822815No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG22370.19825110092726922No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGGTGGTTAACCTGGTATCAGCAGAAA21800.1931995529823187No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC21610.1915157036673352No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21470.1902749725931368No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA21250.18832525233368222No Hit
CCATAGATCAGGAGGTTAGGGGCTTTTCCTGGTTTCTGCTGATACCAGGTTAACCACCTGCTAATACCCTGACTC20970.18584379018528546No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAAAGTTTTTGGAGGGAAAGTGTTAGACTGTT20600.18256471520347547No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20250.17946288751797954No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA20000.17724729631405384No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC19890.17627243618432653No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19810.17556344699907034No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19580.17352510309145872No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT19520.17299336120251654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18950.16794181325756602No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA18840.16696695312783874No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18300.16218127612735928No Hit
ATTGTCAACAGTCTAACACTTTCCCTCCAAAAACTTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTG16990.15057157821878875No Hit
GTAATACATGGCGGTGTCCGAGGCCTCCAGGCTGCTCCACTGAAGGTAGGCGGTGCTGATGGACTTGTCGGCTGA16740.14835598701486305No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16180.14339306271806956No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGC16160.14321581542175552No Hit
GCTAATACCCTGACTCGCCCGACAAGTGATGGTGACTCTGTCTCCTACAGATGCAGACACGGAAGATGGAGACTG16120.1428613208291274No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCC15980.14162058975492903No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG15620.13843013842127608No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15300.1355941816802512No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT14710.1303653864389866No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG14480.128327042531375No Hit
ACCATATACAGCCCGTCCTTCCAAGGCCAGGTCACCATCTCAGCCGACAA14230.1261114513274493No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCA14170.12557970943850716No Hit
ATGTATTACTGTGCGAGACTCAACTATGTTGCGGGGAGTTATTCCCCAAACTACTATTACGGTATGGACGTCTGG14090.12487072025325094No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA13830.12256650540116824No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACATCCAGCTGACCCAGTCTCCATCTTCCGT13430.11902155947488716No Hit
GTATTACTGTGCGAGACTCAACTATGTTGCGGGGAGTTATTCCCCAAACTACTATTACGGTATGGACGTCTGGG13360.11840119393778797No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13300.11786945204884582No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13210.11707183921543256No Hit
GAGTGAAATATGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCGCTTTGCAAACTGGATGCACCATA13190.11689459191911852No Hit
GTATTAGCAGGTGGTTAACCTGGTATCAGCAGAAACCAGGAAAAGCCCCTAACCTCCTGATCTATGGTGCATCCA13010.11529936625229203No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG12760.11308377504836635No Hit
GTCTCCATCTTCCGTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAG12730.11281790410389528No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12690.11246340951126717No Hit
GTTGACAATAGTAAGTTGCAAAATCTTCAGGCTGGAGGCTGCTGATGGTGAGAGTGAAATATGTCCCAGATCCA12650.11210891491863907No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12630.11193166762232501No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC12580.11148854938153986No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA12280.10882983993682907No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12270.10874121628867205No Hit
GTATATGGTATGAGAGTCACCAGGGTAGATGATCCCCATCCACTCCAGGC12030.10661424873290339No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC11940.10581663589949014No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT11890.10537351765870502No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11820.10475315212160581No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT11730.10395553928819257No Hit
GTCTCCTACAGATGCAGACACGGAAGATGGAGACTGGGTCAGCTGGATGTCGCATCTGGAACCTGGGAACCAGA11640.10315792645477934No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA11490.10182857173242393No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11480.10173994808426691No Hit
ACCTTGATGGGACCCCGCTTTGCAAACTGGATGCACCATAGATCAGGAGGTTAGGGGCTTTTCCTGGTTTCT11470.10165132443610987No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT11440.10138545349163881No Hit
CAGTAATACATGGCGGTGTCCGAGGCCTCCAGGCTGCTCCACTGAAGGTAGGCGGTGCTGATGGACTTGTCG11330.10041059336191151No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTA150.0026676937201.0212170
ACATCGT508.0325035E-490.4595470
AATGGTA3150.071.7932970
CTTCAGT9450.060.6254570
GGCGTAT402.3542318E-456.1727369
CACCCAA9750.055.6674170
AAAGTCA2251.4388206E-953.6056670
TTAGACA850.006585659553.21150270
ATTTAAT1552.4534229E-548.63416770
GATGCAT1254.98267E-448.24509470
GTGGTAT2900.046.3819161
TATTCTA2953.074092E-1045.9963870
GTATTGG551.7976527E-742.7978631
GAGCAAA26600.042.50918670
GTTTGGT3203.2392563E-837.69147570
GGAATCA1600.001679336137.69147570
GCTTAAC451.1646915E-437.3632131
CTGACAT5250.037.33251670
ACTCAGT7300.037.17515670
GTATCAA22250.035.667171
Produced by FastQC (version 0.11.5)