FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134539
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC51490.4538407229720618No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT50420.44440957957372995No Hit
CCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTAGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTT38720.34128399288169026No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37800.33317497238966665No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT36780.3241845366267709No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35110.3094649016032063No Hit
TTATTACTGCATGCAATCTCTAGAAACTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCGAACTG33150.2921891622941124No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC32900.2899856241169321No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCGTAAAATCTGTGCCTGACCCACTGCCA32090.2828461604228678No Hit
GTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAAC30330.26733325165551824No Hit
GGTCTAGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTC29690.2616921939219366No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29670.26151591086776216No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG29320.2584309574197097No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28890.2546408717549595No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG28370.2500575123464244No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC28060.2473251250067208No Hit
GAGTAGTACCCATTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGAT27630.24353503934197063No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27610.24335875628779619No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27190.23965681215013324No Hit
GGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGTTCTAA26430.232958056091505No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTC26400.23269363151024336No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT25520.22493717712656858No Hit
GTCAGGGACCCCGAGGGCCCGATTAGAACCCAAATACAATAGGAGTTGTGGAGACTGCCCCGTCTTCTGCAAATA24730.2179739964866787No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24180.21312621249688202No Hit
TGATTAGAGTTTCTAGAGATTGCATGCAGTAATAAAGTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCG23950.21109895737387607No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTAATCTCCAGTCGTGTCCCTTGGCCGAACGTGATTAGAGTT23880.21048196668426558No Hit
GTAATAAAGTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCGTAAAATCTGTGCCTGACCCACTGCCA23640.2083665700341725No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC23400.20625117338407936No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23220.20466462589650952No Hit
GTGCAGCCACAGTTCGGTTAATCTCCAGTCGTGTCCCTTGGCCGAACGTG22000.19391135959186948No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCC21770.19188410446886356No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21470.18923985865624715No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21400.18862286796663666No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGA21220.18703632047906682No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20790.18324623481431665No Hit
GCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCC20640.18192411190800845No Hit
GTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTAGTCAGAGCCTCCTGCA20200.17804588471617105No Hit
GGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATC19150.16879102437201365No Hit
CTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTA19050.1679096091011415No Hit
CTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC19000.16746890146570545No Hit
GACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGCCAGGGAGAGTGGAGACTGAGTCAGCACAA18790.16561792939687398No Hit
CTCCAGGGGTGACGGCCAGGGAGAGTGGAGACTGAGTCAGCACAATGTCCCCACTGGAGCCAGAGACCCAGAGCA18750.16526536328852512No Hit
GTTCAGTGGCAGTGGGTCAGGCACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGGACTTTA18710.16491279718017626No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18690.16473651412600185No Hit
CAATAGGAGTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAG18620.16411952343639136No Hit
CTTTATTACTGCATGCAATCTCTAGAAACTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCGAA18460.16270925900299593No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT18260.16094642846125165No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17930.15803775806737363No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17820.15706820126941426No Hit
CTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCACAGAT17750.15645121057980377No Hit
GCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGT17350.15292554949631526No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG17330.15274926644214082No Hit
GTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGC17060.15036944521078605No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGA16910.14904732230447787No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16740.14754891634399522No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC16670.14693192565438473No Hit
GTCGTGTCCCTTGGCCGAACGTGATTAGAGTTTCTAGAGATTGCATGCAGTAATAAAGTCCAACATCCTCAGCC16570.1460505103835126No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG16310.14375883067924505No Hit
GCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATT16180.14261299082711126No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA16040.14137900944789028No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA16030.1412908679208031No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16030.1412908679208031No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCAC15890.14005688654158208No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15890.14005688654158208No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15650.13794148989148897No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC15600.1375007822560529No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15510.13670750851226798No Hit
GTAGTACCCATTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGC14730.12983246939946536No Hit
CGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAA14620.12886291260150598No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT14600.12868662954733157No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC14590.12859848802024434No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14580.12851034649315712No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT14490.12771707274937222No Hit
GTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGACAGGTTCAGTGGCA14140.12463211930131973No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGGTTAATCTCCAGTCG13910.12260486417831383No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13830.12189973196161613No Hit
CTCCACAACTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCT13780.12145902432618007No Hit
CCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAG13750.12119459974491843No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA13670.1204894675282207No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13630.12013690141987186No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13550.11943176920317415No Hit
TTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGCCAGGGAGAG13520.11916734462191252No Hit
AGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAA13390.11802150476977874No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13330.11749265560725546No Hit
GCCAAGGGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTC13310.11731637255308104No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13240.11669938186347055No Hit
GGCACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGG13200.11634681575512168No Hit
ATCTGTGCCTGACCCACTGCCACTGAACCTGTCAGGGACCCCGAGGGCCCGATTAGAACCCAAATACAATAGGAG13180.11617053270094725No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACAT12940.11405513605085413No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG12810.11290929619872037No Hit
GAGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAATCTCTAGAAACTCTAATCACGTTCGGCCAAGGGA12810.11290929619872037No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12780.11264487161745873No Hit
GTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAGAGTAGTACCCATTGCTATGCAGGAGGCTCTGA12700.11193973940076102No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTC12610.1111464656569761No Hit
CCACTGAACCTGTCAGGGACCCCGAGGGCCCGATTAGAACCCAAATACAATAGGAGTTGTGGAGACTGCCCCG12590.11097018260280167No Hit
CCCAAATACAATAGGAGTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAGAGTAGTACCCATTGCTA12580.11088204107571446No Hit
GATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAATCTCTAGAAAC12570.11079389954862723No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12570.11079389954862723No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG12530.1104413334402784No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12500.11017690885901674No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12500.11017690885901674No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG12490.11008876733192953No Hit
CTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTG12480.11000062580484232No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12340.10876664442562134No Hit
ACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATC12220.10770894610057477No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12140.10700381388387707No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12020.1059461155588305No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT11920.10506470028795838No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11900.10488841723378395No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11800.10400700196291181No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAG11760.10365443585456295No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGC11720.1033018697462141No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC11680.10294930363786525No Hit
GGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGG11370.10021691629816162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTAGA15100.046.3710570
AATGGTA3400.041.18840470
AAAGGGG2559.458745E-1139.2270570
TGGTATC1800.037.516382
GTATCAA14350.036.9509851
GCGTAGA34650.034.64207570
GAGTAAG400.002881792633.773671
GTGGTAT2050.032.9499241
TCGTATG959.3532435E-932.47862642
TCTTATA951.0852091E-831.9876522
ACGTACC652.6880765E-531.170213
TCAACGC16850.031.0609254
CGTATGC1001.5170372E-830.9058643
AACGCAG17000.030.5882326
ATCAACG17250.030.3393333
CAACGCA17150.030.3206965
TATCAAC17450.030.18512
TTGACTA450.00513625330.0131033
CAGTTCG24400.029.92670270
ACGCAGA17800.029.2134787
Produced by FastQC (version 0.11.5)