FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1134539
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT50920.4488166559280906No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA50110.4416771922340263No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC38190.33661249194606796No Hit
CCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTAGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTTTG37050.3265643578581256No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT36680.3233031213558987No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC33330.2937757097816822No Hit
TTATTACTGCATGCAATCTCTAGAAACTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCGAACTGT31790.2802019146102514No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC30890.2722691771724022No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA30230.2664518363846461No Hit
CAGTAATAAAGTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCGTAAAATCTGTGCCTGACCCACTGCCA29570.26063449559689No Hit
GGTCTAGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTC28670.25270175815904083No Hit
GTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGTTCTAATCG28360.24996937081933718No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG28000.24679627584419753No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT27910.24600300210041257No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT27220.23992123673139487No Hit
GGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGTTCTAAT26710.235426018849947No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTG26670.23507345274159813No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC25310.2230862050577371No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24850.2190316948117253No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA24770.21832656259502758No Hit
GTAATAAAGTCCAACATCCTCAGCCTCCACTCTGGCGATTGTTAGCGTAAAATCTGTGCCTGACCCACTGCCA24570.2165637320532833No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT23890.2105701082113528No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23190.2044002013152479No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA22620.1993761342712767No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22270.19629118082322422No Hit
GTCAGGGACCCCGAGGGCCCGATTAGAACCCAAATACAATAGGAGTTGTGGAGACTGCCCCGTCTTCTGCAAATA22190.1955860486065265No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGGTTAATCTCCAGTCGTGTCCCTTGGCCGAACGTGATTAGAGTTT22060.19444020875439277No Hit
GTGCAGCCACAGTTCGGTTAATCTCCAGTCGTGTCCCTTGGCCGAACGTG21950.1934706519564334No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT21920.19320622737517176No Hit
GAGTAGTACCCATTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACG21840.19250109515847405No Hit
TGATTAGAGTTTCTAGAGATTGCATGCAGTAATAAAGTCCAACATCCTCA21460.18915171712915996No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA21270.1874770281145029No Hit
GTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTAGTCA21000.18509720688314812No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20600.1815715457996596No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCC20260.17857473387869435No Hit
GTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTT20160.17769331860782223No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCA20060.17681190333695007No Hit
GTTCAGTGGCAGTGGGTCAGGCACAGATTTTACGCTAACAATCGCCAGAG19310.17020128880540908No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19170.16896730742618807No Hit
GCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCC18900.16658748619483332No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGGTCAGGCACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGAT18870.1663230616135717No Hit
CTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGACAGGTT18850.16614677855939725No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCT18710.16491279718017626No Hit
CTCCAGGGGTGACGGCCAGGGAGAGTGGAGACTGAGTCAGCACAATGTCC18250.16085828693416446No Hit
CTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAGGTCTAGTCAGAGCCTCCTGCATAGCAATGG18230.16068200387999002No Hit
GGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATC18220.1605938623529028No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17900.15777333348611197No Hit
CTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCC17780.1567156351610654No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT17060.15036944521078605No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16930.14922360535865228No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16760.14772519939816967No Hit
CTTTATTACTGCATGCAATCTCTAGAAACTCTAATCACGTTCGGCCAAGGGACACGACTGGAGATTAACCGAA16610.14640307649186146No Hit
GACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGCCAGG16310.14375883067924505No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA16110.14199600013750077No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16040.14137900944789028No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15840.13961617890614603No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA15810.1393517543248844No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA15680.1382059144727506No Hit
GTCGTGTCCCTTGGCCGAACGTGATTAGAGTTTCTAGAGATTGCATGCAGTAATAAAGTCCAACATCCTCAGCCT15630.13776520683731452No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG15310.1349446779705237No Hit
CAATAGGAGTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAGAGTAGTACCCATTGCTATGCAGGAG15270.13459211186217487No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA15260.13450397033508762No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACT15200.13397512117256435No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC15130.13335813048295386No Hit
GCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGTT15100.13309370590169223No Hit
GCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTT14910.13141901688703517No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA14870.1310664507786863No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC14770.13018503550781418No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT14750.13000875245363974No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC14130.12454397777423253No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT14030.1236625625033604No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA13970.12313371334083713No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT13890.1224285811241394No Hit
ATCTGTGCCTGACCCACTGCCACTGAACCTGTCAGGGACCCCGAGGGCCC13770.12137088279909283No Hit
GTAGTACCCATTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGC13690.12066575058239513No Hit
CCACTCTCCCTGGCCGTCACCCCTGGAGAGCCGGCCTCCATCTCTTGCAG13680.12057760905530793No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13580.1196961937844358No Hit
CTCCACAACTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGACAGGTTCAGTGGCAGTGGGTCAG13400.11810964629686595No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT13260.11687566491764495No Hit
CGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT13190.11625867422803447No Hit
CTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGAC13050.11502469284881349No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG12980.11440770215920298No Hit
AGTCAGAGCCTCCTGCATAGCAATGGGTACTACTCTTTGGATTGGTATTTGCAGAAGACGGGGCAGTCTCCACAA12910.11379071146959249No Hit
GAGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAATCTCTAGAAACTCTAATCACGTTCGGCCAAGG12900.11370256994250529No Hit
GTATTTGCAGAAGACGGGGCAGTCTCCACAACTCCTATTGTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCT12830.1130855792528948No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGG12810.11290929619872037No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGGTTAATCTCCAGTCGT12640.11141089023823773No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT12540.11052947496736559No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG12460.1098243427506679No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTC12380.10911921053397017No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA12220.10770894610057477No Hit
GGCACAGATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAATCTCTA12220.10770894610057477No Hit
GTTGTGGAGACTGCCCCGTCTTCTGCAAATACCAATCCAAAGAGTAGTACCCATTGCTATGCAGGAGGCTCT12170.10726823846513871No Hit
GCCAAGGGACACGACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTC11980.10559354945048165No Hit
GTATTTGGGTTCTAATCGGGCCCTCGGGGTCCCTGACAGGTTCAGTGGCA11890.10480027570669671No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGT11890.10480027570669671No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA11870.10462399265252231No Hit
GGGTCTCTGGCTCCAGTGGGGACATTGTGCTGACTCAGTCTCCACTCTCCCTGGCCGTCACCCCTGGAGAGCCG11730.10339001127330132No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT11630.10250859600242919No Hit
ACTGGAGATTAACCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTG11570.10197974683990589No Hit
GATTTTACGCTAACAATCGCCAGAGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAATCTCTAGAAACT11560.10189160531281868No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGG11560.10189160531281868No Hit
TTGCTATGCAGGAGGCTCTGACTAGACCTGCAAGAGATGGAGGCCGGCTCTCCAGGGGTGACGGCCAGGGAGAGT11420.1006576239335977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGCGA150.002072654271.2619961
AATGGTA3250.060.13660470
AGTAGGT4550.052.8673470
TAAGTCA1204.1310865E-450.11383470
CGCCCTA307.111509E-444.899037
GAGCAAA35500.040.6557370
GATCAGT1500.001239981340.09106470
GTATCAA15100.037.270331
GTGGTAT2400.036.5078121
GCCACAA1650.00198052436.44642370
TGGTATC2500.033.6757552
AGAGCAT12550.033.54232470
ACATCCA2302.552081E-432.68293470
CTCGTAT1857.959265E-532.5062770
ATCAACG17550.031.0825713
GCACAAT15300.030.46135170
TCAACGC18000.030.3068434
AACGCAG18550.029.9528546
CAACGCA18250.029.8916825
ACTTCCA4552.1929736E-829.73787970
Produced by FastQC (version 0.11.5)