FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685377

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685377
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1151886
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34670.30098464605004316No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT29870.25931385571141585No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA29420.25540721911716957No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC27060.23491908053401117No Hit
GTACTAGAAGTCGGCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGA25740.22345961319088867No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA21970.19073067994575854No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT21400.18578227359304653No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC20780.18039979650764051No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20480.1777953721114763No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG19630.17041616965567774No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGTATGCCATAACTACTGAAGCTGAATCCAGACGCTGCACA18610.16156112670871944No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT18390.1596512154848657No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTGGGAGACAGAGTCACCATCACTT18040.15661272035600746No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18020.15643909206292983No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17110.1485390047278984No Hit
GATCTATGCTGCATCCACTTTGCAATCAGGGGTCCCGTCTCGGTTCAGTGGCGGTGGATCTGGGACAGATTTCA16580.14393785496134168No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT16440.14272245690979837No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC16100.13977077592747894No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16100.13977077592747894No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16080.13959714763440131No Hit
GCATAGATCAGGAGCTTAGGAACTTTCCCTGGTTTCTGCTGATACCAGGCTAAAAAATTGCTAATGCCCTGACTC15750.1367322807986207No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC15730.13655865250554308No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGT15350.13325971493706842No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG15120.13126298956667587No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA14680.12744316711896836No Hit
CTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCA14570.12648821150704148No Hit
CTCCTGTGCAGCGTCTGGATTCAGCTTCAGTAGTTATGGCATACACTGGGTCCGCCAGGCTCCAGGCAAGGG14140.12275520320587281No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCGACAGCACCCCCCAAGATGGGAACGTGGTCGTCGCATGCCTGGTCCAGG14010.12162661930086832No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGACCCTTGGCCGAACGTC13910.12075847783548026No Hit
GTCGCTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC13850.12023759295624739No Hit
GTCTGCATCTGTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGG13640.11841449587893246No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC13530.11745954026700559No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCAGATGTGACAT13530.11745954026700559No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA13510.11728591197392797No Hit
GAGTGAAATCTGTCCCAGATCCACCGCCACTGAACCGAGACGGGACCCCTGATTGCAAAGTGGATGCAGCATA13480.11702546953431155No Hit
GTAATAAGTTGCAACATCTTCAGGCTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACCGCCA13430.11659139880161751No Hit
ATAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATCCACCGTGTTCTTGGAATTGTCTCTGGA13400.11633095636200111No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGG13340.11581007148276826No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAG13130.11398697440545333No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCTGGTGCCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC13100.11372653196583689No Hit
GTGGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGGGCATTAGCAATTTTTTAGCCTGGTATCAGCAGAAA13080.11355290367275929No Hit
GTCTATTATTGTACGAGAGAAAGTGACCCGGGCGGTCACGCTCCATTTGA13010.11294520464698764No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA12840.11146936415582794No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG12740.11060122269043984No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT12720.11042759439736224No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGACTCCTGCTGCTCTGGCTCCCAGATACCA12580.10921219634581894No Hit
GTATTTATTACTTCCATCATACCATATAACTACCACCCACTCCAGCCCCT12500.10851768317350849No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT12460.10817042658735326No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCCAGACGGCAAGTCCGTGACATGCCACGT12260.10643414365657713No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12020.10435060413964577No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCG11920.1034824626742577No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT11840.10278794950194725No Hit
GTGTATGCCATAACTACTGAAGCTGAATCCAGACGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGACCAC11760.10209343632963679No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT11710.10165936559694275No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTTCAGGGCCAGCTCCTCCGACGGCGGC11580.10053078169193826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA5800.067.8602870
CGATTAA800.005920022754.66522670
AAGCGGT10800.049.9410770
TTTGACA11300.046.44125770
CAGCCAT15200.046.03387570
AAAGTCA4600.041.19698370
TAGGCAA4300.040.681170
TAGCAAT14500.040.213570
GAGCAAA21500.039.32506670
GTGTTCA4157.2759576E-1238.63887870
GTATCAA26200.038.026571
CCGCTAT1550.00169544437.6190870
ATGGCAA2357.959337E-637.2188870
TAGACTG656.652281E-736.2210275
CCCAAAT1700.002667834734.2997570
GTGGTAT3150.034.1929131
CTAGACT400.002940399833.6352734
GAGGGTT2202.3565529E-433.13044470
TATCAAC29800.032.7351572
ACCAGGT4955.820766E-1132.3942170
Produced by FastQC (version 0.11.5)