FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628810
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGGTAGCTCACCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG26840.42683799557895075No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT19320.3072470221529556No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17070.2714651484550182No Hit
CAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA16480.26208234601867014No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA15370.24442995499435444No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT14790.235206183107775No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14480.23027623606494807No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13550.21548639493646732No Hit
CTCTGTGAGTGGTCGATTCACCATCTCCAGAGACAACGCCAAGAACTCAC13090.2081709896471112No Hit
ATAATAAACAGCCGTGTCCTCGTCTCTCAGGCTGTTCATTTGCAAAAACA12960.20610359250011925No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTCAGGTACAGCTGGTGGAG12550.19958333995960623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT12490.1986291566609946No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12330.1960846678646968No Hit
GTATGGTAGCTCACCTCGGACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA12290.1954485456656224No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT11600.18447543773158825No Hit
GTTTATTATTGTGCGAGTACAGCAAGACTTGACTGGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCC11520.18320319333343935No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTAATTATGAAATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG11000.17493360474547162No Hit
CATCTATGGTGCATCAAGCAGGGCCATTAACATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA10900.1733432992477855No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10650.16936753550357023No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA10520.1673001383565783No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTGGGGCTGTGCTGGGTTTTC10370.16491468011004914No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10320.1641195273612061No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT10210.16237019131375138No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTTACAGCAGCTATTTAGCCTGGTACC10080.16030279416675944No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC10040.159666671967685No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG9970.1585534581193047No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT9960.15839442756953612No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG9820.15616799987277555No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC9800.15584993877323833No Hit
ACCATGTACTACGGAGACTCTGTGAGTGGTCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTTTT9690.15410060272578363No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9690.15410060272578363No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG9650.1534644805267092No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG9630.15314641942717194No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTATTACTGTCAGCAGTA9590.1525102972280975No Hit
TGGTAATACCATGTACTACGGAGACTCTGTGAGTGGTCGATTCACCATCTCCAGAGACAACGCCAAGAACTCA9520.15139708337971725No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC9520.15139708337971725No Hit
CTCACAGAGTCTCCGTAGTACATGGTATTACCAATACTACTAATAGATGA9150.14551295303827866No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG9120.14503586138897281No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC9030.14360458644105534No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT8990.1429684642419809No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG8980.14280943369221227No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTTACAGCAGCTATTTAGCC8940.14217331149313783No Hit
GTACTACGGAGACTCTGTGAGTGGTCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTTTTTGCAAA8840.14058300599545173No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTTACAG8830.14042397544568314No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA8790.1397878532466087No Hit
GGGTTCCCTGGCCCCACCAGTCAAGTCTTGCTGTACTCGCACAATAATAAACAGCCGTGTCCTCGTCTCTCAGG8760.13931076159730285No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8700.1383565782986912No Hit
GTGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTCAGGTACAGCTGGTGGAGTCTGGGGGAGGCT8610.13692530335077369No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT8600.13676627280100506No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCTGGTACCAGGCTAAATAGCTGCTGTAAACACTCTGA8530.1356530589526248No Hit
GTATTACCAATACTACTAATAGATGAAACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATTTCA8460.13453984510424452No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT8440.1342217840047073No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACCAGTCAAGTCTTGCTGTA8430.1340627534549387No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC8400.13358566180563286No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8390.13342663125586426No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTTTTTGCAAATGAACAGCCTGAGAGACGAGGACACGGCTGTTTATTA8340.1326314785070212No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC8270.13151826465864092No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTTAATGGCCCTGCTTGATGCACCATAGATGAGGAGCC8180.13008698971072344No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC8160.12976892861118622No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8030.12770153146419427No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA8020.12754250091442568No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT7980.12690637871535124No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG7900.12563413431720233No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA7730.12293061497113596No Hit
GCCTGAGAGACGAGGACACGGCTGTTTATTATTGTGCGAGTACAGCAAGACTTGACTGGTGGGGCCAGGGAACC7710.12261255387159874No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC7660.12181740112275569No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG7660.12181740112275569No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT7650.12165837057298706No Hit
GAGTACGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGATTCCAAGGCATTTCCACTTGGTGATCAGCACTGAACA7550.12006806507530096No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGA7530.11975000397576376No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC7450.11847775957761486No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7430.11815969847807765No Hit
CTACCATACTGCTGACAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC7360.11704648462969737No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC7330.11656939298039153No Hit
GTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGT7290.11593327078131709No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGTTAATGGCCCTGCTTGATGCACCATAGA7230.11497908748270542No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG7180.11418393473386237No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC7110.11307072088548209No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT7080.11259362923617627No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTC6850.10893592659149823No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCCAG6820.1084588349421924No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCAAGCAGGGCCATTAACATCCCAGACAGGT6760.10750465164358074No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT6750.10734562109381211No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCC6660.10591434614589462No Hit
GTATCAACGCAGAGTACGGGAGCTCTCAGAGAGGTGCCTTAGCCCTGGAT6540.1040059795486713No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAATGTATTACTGTCAGCAGTATGGTAGCTCA6530.1038469489989027No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6510.10352888789936547No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAGTATGCTGGTCACAGCGAAGGTGG6410.10193858240167937No Hit
GTCAGGTACTTCTCGCGGGGCAGCTCCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG6360.10114342965283632No Hit
GTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGGACACTGGACACGCTGTAGCAGCCA6360.10114342965283632No Hit
ACCATACTGCTGACAGTAATACATTGCAAAATCTTCAGGCTCCAGTCTGC6330.10066633800353048No Hit
CCTGTGACCCCTGCAGCCAGCGAACCAGCACGTCCTTGGGGCTGAAGCCG6320.10050730745376187No Hit
CTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAATTCATTTCATAATTAC6290.10003021580445604No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG450.002717532566.53776670
TCGTATG1800.053.30476440
ATCTCGT1750.050.81794437
CGTATGC1900.050.52350641
ACTAGCG1850.049.741127
ATGCCGT1950.049.3109544
CCGTCTT1900.048.9424747
CTTATAC1950.048.8991431
TATACAC1950.048.891373
TACACAT1950.048.891375
TGTTAGA3500.048.47751270
TAGCGCT1900.048.4514129
CTAGCGC1900.048.44754828
CACGAGA2000.047.67666621
TCTCCGA2000.047.6652911
GCCGTCT1950.047.64920846
CATCTCG1800.047.47196236
CGTCTTC1900.047.16013348
CGCTCAT1900.046.7015432
TTATACA2050.046.506422
Produced by FastQC (version 0.11.5)