FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1065348
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGATTCTTGGACTTGTCTAGT44970.42211559039863034No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37150.34871234563729414No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33130.3109781967957888No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA31810.29858787926574226No Hit
GAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT29640.27821894817468096No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25580.24010933516559846No Hit
GTATATTACTGTGCGAGGAGGTATTCGTCGGGTGAATTTAACTTCTACATGGACGTCTGGGGCAAAGGGACCACG23430.21992813615832574No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT21890.2054727657066048No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20920.1963677596428585No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC20910.1962738936009642No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19630.18425904023849485No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGTAACTGGTGGAGTTGGGTCCGCCAGCCCCCAGGGAAGGGGCTGGAGTG19400.1821001212749261No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA18940.1777822833477887No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18370.17243191895981408No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17640.16557969790153076No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA17620.16539196581774218No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC16340.15337711245527283No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA16100.15112432744980983No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCAGTAGCA15900.149247006611924No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG15590.14633715931320093No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCAGTAGCAGCTATTTAAATTGGTATCAGCAGAAA15470.14521076681046943No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGT15410.1446475705591037No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT15400.1445537045172094No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15350.14408437430773793No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC15350.14408437430773793No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC15100.14173772326038064No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14560.13666895699808887No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14530.13638735887240602No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG14520.1362934928305117No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC14470.13582416262104025No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG14210.1333836455317887No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14100.13235111907095146No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13760.12915967364654554No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTACTGCTCTGACTT13410.12587436218024534No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT13380.12559276405456246No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13190.12380930925857091No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCGAGGGGTA13050.12249518467205084No Hit
CAGTAATATACGGCCGTGTCCGCGGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGATTCTTGGACTTGTCTAGT12880.12089946195984785No Hit
AGTCAGAGCAGTAGCAGCTATTTAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATCTAT12860.12071172987605928No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTCTCCATCCTCCCTGTCTGCATCTGTA12840.12052399779227069No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGGCTCCATCAGCAGTAGTAACTGGTGGAGTTGGGTCCGCCAGCCCCCAGG12750.11967920341522206No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12740.11958533737332779No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12590.1181773467449134No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACAT12310.11554909757187323No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACC12300.11545523152997894No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC12050.11310858048262165No Hit
GTACTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAAT12030.11292084839883305No Hit
GAGTGAAATCTGTCCCAGATCCACTGCCACTGAACCTTGATGGGACCCCACTTTGCAAACTGGATGCAGCATA11590.10879074255548421No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11530.10822754630411847No Hit
ACTTACTACTGTCAACAGAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA11390.10691342171759838No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11370.10672568963380981No Hit
GTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA11340.10644409150812692No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG11210.10522383296350114No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTCCCAGATCCACTGCCA11100.10419130650266392No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA10840.10175078941341233No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC10830.10165692337151803No Hit
GCTACAACCCGTCCCTCAAGAGTCGAGTCACCATATCACTAGACAAGTCCAAGAATCAGTTCTCCCTGAAGCT10810.10146919128772947No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10770.10109372712015227No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10750.1009059950363637No Hit
GATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTACTGCTCTGACTTGCCCG10720.10062439691068083No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10690.10034279878499794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA559.272391E-6107.8475670
TACTTTA700.003260164563.55302470
AATGGTA3900.060.83708670
CGTTCCA750.004284732559.3161670
CATCGAA1204.420377E-449.43013470
GACCCAT3950.045.05024370
GACAGGT3150.043.076831
ACCAGGT2451.937351E-742.36868370
GTAGTTT1755.5394896E-542.36868370
GTATCAA24600.040.817921
AAGGACA18450.038.57961370
GTGGTAT2650.038.40341
GGACCCA11350.036.58265370
AGTTAAA9600.035.5279170
AAAGTCA2958.307834E-735.1875570
AAACTCA2551.2386015E-534.89185770
AAGCGGT10250.034.72165370
CCGTATA7700.034.66528370
CTCGACA400.002601168834.48663347
ATCAACG29000.034.2546273
Produced by FastQC (version 0.11.5)