FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727779
Sequences flagged as poor quality0
Sequence length19-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCTTGGTCCCAG16850.23152632873440973No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG16570.227679006951286No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT15290.21009125022843472No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC15080.20720575889109197No Hit
ATAATACACAGCCGTGTCGTCGGCTCTCAGACTGTATATTTGTAGATACAACATATTCTTGGCGTTGTCTC14250.19580119789111805No Hit
GATTATTACTGCAGCTCATATACAAGCAGAAACACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCTTAGG14180.1948393674453371No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCTCC14060.1931905152525698No Hit
GACCTAAGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTTCTGCTTGTATATGAGCTGCAGTAATAA13730.18865617172245971No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG13660.18769434127667878No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG13560.18632029778270603No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC13250.18206076295139045No Hit
GTACCAACACCACCCAGGCCAAGCCCCCAAACTCATTATTTTTGAGGTCATTAATCGGCCCTCAGGGGTCTCTAA11680.16048828009601815No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT11550.15870202355385357No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG11410.1567783626622917No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTTGTTGCTATTTTAAAAGG11290.15512951046952442No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC11180.15361806262615438No Hit
GTGTATTATTGTGCAAGAGATGGATTACCAGGTGCTCGTGACGTCTGGGGCCAGGGAACCCTGGTCACCGTCTC11140.15306844522856525No Hit
GTGTTGGTACCAGGAGACATAGTTATAAACACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGCGATCGA11000.1511447843370034No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTT10900.14977074084303066No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10150.13946541463823495No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC10130.1391906059394404No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10070.13836617984305674No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAGG9770.13424404936113848No Hit
CTCCTGGACAGTCGATCGCCATCTCCTGCACTGGAACCAGCAGTGACGTT9720.1335570276141521No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGTTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCCAAGC9460.1299845145298229No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC9410.12929749278283656No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCAGAGAAACGATTAGAGACCCCTGAGGGCCGAT9390.129022684084042No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9370.12874787538524746No Hit
GCCTTGGGCTGACCTAAGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTTCTGCTTGTATATGAGC9330.12819825798765835No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT8970.1232517014093564No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC8570.11775552743346537No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA8440.11596927089130078No Hit
GCCCTGGGATTCCCAGCTGTTTCTGCTTGCTGATCAGGACTGCACACAGA8370.11500744044551986No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG8320.11432041869853349No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG8300.11404560999973892No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG8300.11404560999973892No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8230.113083779553958No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8150.1119845447587798No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT8100.11129752301179342No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8020.1101982882166152No Hit
CAATAATACACAGCCGTGTCGTCGGCTCTCAGACTGTATATTTGTAGATACAACATATTCTTGGCGTTGTCTC7930.10896164907203973No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCTCCTGCACTGGAACCAGCAGTGACGT7770.1067631794816833No Hit
GTATATTTGTAGATACAACATATTCTTGGCGTTGTCTCTGGACATGGTGAATCGGCCCCTCACGGAGTCCGCGTA7730.10621356208409422No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG7710.10593875338529965No Hit
GGCCAAGCCCCCAAACTCATTATTTTTGAGGTCATTAATCGGCCCTCAGG7680.10552654033710784No Hit
GTTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCCAAGCCCCCAAACTCATTATTTTTGAGGTCATTAA7680.10552654033710784No Hit
GTATTATTGTGCAAGAGATGGATTACCAGGTGCTCGTGACGTCTGGGGCC7660.10525173163831328No Hit
CCTTGGGGCTGGTCGGGGATGGTGAGGAGACGGTGACCAGGGTTCCCTGG7560.10387768814434052No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC7530.10346547509614869No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC7410.10181662290338139No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG7340.10085479245760046No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG7330.10071738810820319No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACGA150.008739521135.1855370
AGGGGGG2300.079.3480370
ATCGCGT408.7712215E-751.30257437
TGACGAA600.0079761250.69457670
TGCATAG600.0079761250.69457670
CGTATGC851.8189894E-1244.39261641
AATGGTA1651.7206366E-743.0135870
CTACGCT1850.040.56884828
TCTCGTA857.2759576E-1140.27895738
TGCATCG603.142377E-739.855334
GGTATCA5200.039.3468971
ACTACGC2000.039.2319227
CGAGACT2000.039.21574423
ACGAGAC2000.039.21574422
GTGGTAT1700.038.1124841
TCTCGCG6950.037.92975270
TGGTATC1800.035.9852332
GTATCAA12000.035.8056761
ATGCCGT1250.035.74966444
TACGCTG2100.035.73922329
Produced by FastQC (version 0.11.5)