FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727779
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19090.2623049029993995No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT16260.22341947211997046No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15390.21146529372240747No Hit
TCTCTGGACTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGAAACACTTATGTCTTCG15040.20665614149350284No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC14530.19964851967424177No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCTCCT13570.1864577021321033No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC13070.1795874846622395No Hit
GACCTAAGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTTCTGCTTGTATATGAGCTGCAGTAATAAT12590.17299207589117027No Hit
GATTATTACTGCAGCTCATATACAAGCAGAAACACTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCTTAGGT12530.1721676497947866No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG12460.17120581934900567No Hit
ATAATACACAGCCGTGTCGTCGGCTCTCAGACTGTATATTTGTAGATACAACATATTCTTGGCGTTGTCTCTG12320.16928215845744382No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT11860.16296155838516913No Hit
GTGTATTATTGTGCAAGAGATGGATTACCAGGTGCTCGTGACGTCTGGGGCCAGGGAACCCTGGTCACCGTCTC11560.15883942790325084No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGC10810.14853410169845516No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTT10780.14812188865026332No Hit
CTCCTGGACAGTCGATCGCCATCTCCTGCACTGGAACCAGCAGTGACGTT10470.14386235381894777No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG10280.14125167118039955No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG9650.13259519716837118No Hit
GTGTTGGTACCAGGAGACATAGTTATAAACACCAACGTCACTGCTGGTTCCAGTGCAGGAGATGGCGATC9550.1312211536743984No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG9490.13039672757801474No Hit
GTACCAACACCACCCAGGCCAAGCCCCCAAACTCATTATTTTTGAGGTCA9490.13039672757801474No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC9320.12806085363826106No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGTTTATAACTATGTCTCCTGGTACCAACACCACCCAGGCCAAGC9040.12421353185513734No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG8940.1228394883611646No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8860.12174025356598638No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8670.11912957092743814No Hit
GCCTTGGGCTGACCTAAGACGGTGACCTTGGTCCCAGTTCCGAAGACATAAGTGTTTCTGCTTGTATATGAGCT8530.11720591003587628No Hit
GTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCAGAGAAA8530.11720591003587628No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT8160.11212194910817706No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC8100.11129752301179342No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC8070.11088530996360159No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA8040.11047309691540977No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCGCCATCT7990.10978607516842337No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7800.10717539252987514No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGTCCAGAGATGGTCAGGGAGGCCGTGTTGCCAAACTTGGAGCCAGA7790.10703798818047787No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA7780.10690058383108059No Hit
GTATATTTGTAGATACAACATATTCTTGGCGTTGTCTCTGGACATGGTGA7780.10690058383108059No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC7630.10483951859012144No Hit
GGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7590.10428990119253234No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG7520.10332807074675142No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG7500.10305326204795685No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT7480.10277845334916232No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGCGT200.008099136138.6609670
CGGTACG200.00705378952.30536759
TAACCGG200.007760142451.05803314
CGGTCTA200.007766469451.0475110
CGGCAAA1402.2020007E-549.52177470
CAAGCTT1309.122678E-442.66491370
AAGTAAT1350.001098434641.08473270
CCTGCAA1350.001098434641.08473270
GTACGGA350.001430932238.9495431
GGTATCA5150.038.3823171
GAGCCAT8350.038.19404270
GTATCAA12100.038.024091
CGAGGGT1302.7812348E-936.7640169
AAAAGGT8550.035.6788570
GTGGTAT1600.034.080851
TCTAGCA2052.0854032E-433.8197570
TATCAAC13350.033.6516082
GACAGGT2400.032.6608161
ACTCAGT7350.032.07124770
ATCAACG14150.031.744683
Produced by FastQC (version 0.11.5)