FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614357
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC195061.2082829262672383No Hit
CCCATATACAGCGCACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC128450.7956728282529824No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAG75510.46774040686167934No Hit
GATTATTACTGTGCGCTGTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGG70940.439431922431036No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG66840.41403481386087465No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAGCGCA53440.33102962975351796No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG53420.3309057414190294No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAGCGCACAGTAATAA47790.2960311752604907No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG46580.2885359310239309No Hit
GTGGTGACCCAGGAGCCATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTC45790.2836423418116315No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC44670.27670459508027034No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCA43320.2683421325022904No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCT43090.2669174166556716No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG40550.25118359817562036No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT39790.24647584146505389No Hit
GTAATACACAGCCGTGTCCTCGGGAGTTACAGAATTCAGCTGTAGGGAGAACTGGTTCTTGGATGTGTCTGGG39790.24647584146505389No Hit
ACCCATATACAGCGCACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGT39170.24263530309590753No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAGG37720.2336533988454846No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT37170.23024646964704834No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36740.2275828704555436No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGGAGATGAGCGTGCGCGGAGCCTGG35080.21730013869299047No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC33260.20602630025452856No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG32960.2041679752371997No Hit
CAGTAATACACAGCCGTGTCCTCGGGAGTTACAGAATTCAGCTGTAGGGAGAACTGGTTCTTGGATGTGTCTGGG32390.20063715770427484No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC32350.20038938103529766No Hit
CCCCAGGCCAGGCTCCGCGCACGCTCATCTCCAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTC32050.19853105601796875No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG31530.19530995932126538No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGC30970.1918410859555848No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29590.18329279087587194No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28790.17833725749632826No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTTCCAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC28780.17827531332908397No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC28770.17821336916183966No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG27620.17108978992874563No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGG27520.17047034825630267No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC27310.16916952074417244No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTTCCAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGG27170.16830230240275232No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC26080.16155038817312403No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG24890.1541790322710528No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGAT24760.15337375809687698No Hit
CTCATCTCCAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAG24740.15324986976238839No Hit
GTGTATTACTGTGCGCGAACACACAACTATGCTATTGACAACTGGGGCCA24590.15232070725372393No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGT23970.14848016888457757No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG23760.14717934137244737No Hit
GTCTCTTCCAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC23660.1465598997000044No Hit
CCCAAATGCCACTACCCATATACAGCGCACAGTAATAATCAGATTCATCA23360.14470157468267553No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA23310.14439185384645403No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23210.1437724121740111No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACT23120.14321491466881242No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAG22930.14203797549117078No Hit
CCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCCAGGAC22850.1415424221532164No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTGGAAGAGACTGAGCCAGAGCTCAAGCCACAAGTGAGTG22720.14073714797904058No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCT22610.1400557621393533No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACA22320.13825938128926873No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTAC22090.1368346654426499No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22030.13646300043918413No Hit
GTATTACTGTGCGCGAACACACAACTATGCTATTGACAACTGGGGCCAGG21750.13472856375634387No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAA21550.13348968041145792No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC21460.13293218290625927No Hit
ATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTTCCAGTTA21300.13194107623035053No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGAT21050.13039247204924312No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGCGCTGTATATGGGTAGTGGCATTTGGG20150.12481749699725649No Hit
GAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTGGAAGAGACTGAGCCAGAGCTCAAGCCACAAG19370.1199858519522014No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19300.11955224278149133No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19270.11936641027975843No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19260.11930446611251415No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG19220.11905668944353696No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG19220.11905668944353696No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT19210.11899474527629268No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC19160.11868502444007119No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCT18950.11738419692794097No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTTCCAGTTACTA18870.1168886435899866No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGAGCGAGTGTT18740.11608336941581075No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC18550.11490643023816913No Hit
GAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGCGCTGTATATGGG18410.11403921189674898No Hit
GGAGATGAGCGTGCGCGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG18270.11317199355532885No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTG18170.11255255188288588No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTG18110.1121808868794201No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG17980.11137561270524426No Hit
GCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGA17860.1106322826983127No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCA17470.10821646017578515No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCC17380.10765896267058649No Hit
GGCCAGGCTCCGCGCACGCTCATCTCCAGCACAAACACTCGCTCTTCTGG17240.10679174432916634No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT17140.1061723026567234No Hit
GTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCC17030.10549091681703612No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC16640.10307509429450859No Hit
GCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAGCGCACAGTAATAATCAGATTCATCATC16580.10270342929104281No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGA16500.10220787595308845No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16380.10146454594615688No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)