FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685387

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685387
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1614357
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC189061.1711164259206608No Hit
CCCATATACAGCGCACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTC112350.6959427189896659No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT64300.3983009953808235No Hit
GATTATTACTGTGCGCTGTATATGGGTAGTGGCATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGT63590.3939029595064784No Hit
GTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCCAGA62900.389628811966622No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG54420.337100158143459No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAGCGCA48850.30259725698838613No Hit
GTGGTGACCCAGGAGCCATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTG46160.2859342759996704No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45640.2827131793029671No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTACCCATATACAGCGCACAGTAATAAT41780.2588027307466688No Hit
GGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCAT41650.25799745657249296No Hit
CACTTAGCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGA40150.24870583148584854No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC39520.2448033489494579No Hit
ACCCATATACAGCGCACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTT37550.2326003480023316No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT36950.2288836979676738No Hit
GTAATACACAGCCGTGTCCTCGGGAGTTACAGAATTCAGCTGTAGGGAGAACTGGTTCTTGGATGTGTCTGG36300.22485732709679457No Hit
CTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAAGCTGCCCTCACCATCACGGGGGCCCAG36060.22337066708293146No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT35660.22089290039315965No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG35250.2183531895361435No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTA33660.20850406694430043No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA32910.2038582544009782No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32140.19908855352316746No Hit
CCCCAGAAGAGCGAGTGTTTGTGCTGGAGATGAGCGTGCGCGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG31220.1933896901366922No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC31130.19283219263149354No Hit
CCCCAGGCCAGGCTCCGCGCACGCTCATCTCCAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTG30840.19103581178140894No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30700.1901685934399888No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG30490.1888677659278586No Hit
CAGTAATACACAGCCGTGTCCTCGGGAGTTACAGAATTCAGCTGTAGGGAGAACTGGTTCTTGGATGTGTCTGG29190.1808150241861001No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGG29140.18050530334987863No Hit
GGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGA28430.1761072674755336No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG28000.17344366828402888No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC27890.1727622824443416No Hit
CTTGTGGCTTGAGCTCTGGCTCAGTCTCTTCCAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGCTC27330.16929340907866103No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCT26760.16576259154573617No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26610.16483342903707174No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTG26380.16340871319045291No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG25940.1606831698317039No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA25830.16000178399201664No Hit
CACTTGTGGCTTGAGCTCTGGCTCAGTCTCTTCCAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAG25200.156099301455626No Hit
CTCATCTCCAGCACAAACACTCGCTCTTCTGGGGTCCCTGATCGCTTCTCTGGCTCCATCGTTGGGAACAAA25180.1559754131211374No Hit
GCCTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACT24500.15176320974852525No Hit
GTGTATTACTGTGCGCGAACACACAACTATGCTATTGACAACTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCA24400.1511437680760823No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT24240.15015266140017358No Hit
GTCTCTTCCAGTTACTACCCCAGCTGGTACCAGCAGACCCCAGGCCAGGC24230.15009071723292927No Hit
CCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTC24160.1496571080622192No Hit
GGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCCTTCTCAGTGTC23690.14674573220173728No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG22690.1405513154773077No Hit
GGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTGGAAGAGACTGAG22630.1401796504738419No Hit
TTCTCAGACTGTGGTGACCCAGGAGCCATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTT22350.1384452137910016No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTT22200.13751605128233718No Hit
ATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTTCCAGTTA22190.13745410711509287No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22060.13664883294091704No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT22030.13646300043918413No Hit
GATCAGGAGTGGATTCTCAGACTGTGGTGACCCAGGAGCCATCCTTCTCAGTGTCCCCTGGAGGGACAGTCACA21840.13528606126154252No Hit
CTTCATGAGTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATT21200.13132163455790757No Hit
CCCAAATGCCACTACCCATATACAGCGCACAGTAATAATCAGATTCATCATCTGCCTGGGCCCCCGTGATGGTGA20580.12748109618876122No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG20340.1259944361748981No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCACTAC20320.12587054784040955No Hit
CCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCCAGGAC20220.12525110616796656No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20160.1248794411645008No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC19650.1217202886350417No Hit
CACCATCACGGGGGCCCAGGCAGATGATGAATCTGATTATTACTGTGCGCTGTATATGGGTAGTGGCATTTGGGT19620.12153445613330881No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC19490.12072918195913296No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC19260.11930446611251415No Hit
GTATTACTGTGCGCGAACACACAACTATGCTATTGACAACTGGGGCCAGG19260.11930446611251415No Hit
GTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAAGAGCGAGTGTTT18610.1152780952416349No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC18570.11503031857265772No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA18400.11397726772950469No Hit
GAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGGGGTAGTAACTGGAAGAG18290.11329588188981744No Hit
GTCACACTCACTTGTGGCTTGAGCTCTGGCTCAGTCTCTTCCAGTTACTACCCCAGCTGGTACCAGCAGACCCCA17990.11143755687248855No Hit
GGAGATGAGCGTGCGCGGAGCCTGGCCTGGGGTCTGCTGGTACCAGCTGG17970.11131366853799995No Hit
GTGTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCA17660.10939339935342678No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17610.1090836785172053No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGG17610.1090836785172053No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAA17570.10883590184822811No Hit
GGCCAGGCTCCGCGCACGCTCATCTCCAGCACAAACACTCGCTCTTCTGG17490.10834034851027374No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTG17420.10790673933956368No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17420.10790673933956368No Hit
GTCCCCACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTG17380.10765896267058649No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATGCCA17370.1075970185033422No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAG17370.1075970185033422No Hit
GAACAAAGCTGCCCTCACCATCACGGGGGCCCAGGCAGATGATGAATCTG17310.10722535349987643No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTAT17220.10666785599467775No Hit
GCCTGGGCCCCCGTGATGGTGAGGGCAGCTTTGTTCCCAACGATGGAGCCAGAGAAGCGATCAGGGACCCCAGAA16770.10388036846868444No Hit
ACCATGGCCTGGATGATGCTTCTCCTCGGACTCCTTGCTTATGGATCAGGAGTGGATTCTCAGACTGTGGTGACC16180.10022566260127097No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT507.802813E-491.1305670
CGATTAA550.001139410182.8459670
GCGATTT550.001139410182.8459670
AATGGTA5400.059.0661170
CGTGCGT800.005033639756.95660470
CGCTCTA200.006848097352.7012661
CTCAGCA10900.048.7701870
GGTAGAA42050.048.03949770
TGACAGT32800.046.30618370
GTATAAC306.834109E-445.2642941
GGTATCA12250.045.1719171
CTCCCTA7200.042.19007570
TGGTATC3650.040.8959962
ATGGCAA3501.537046E-939.05595470
CCTCTCA17650.038.72403770
AAGCTTT2051.2231761E-437.0449570
GTCTAGA558.261213E-637.0344241
AAGCGGT9050.036.92214670
TTGCAAT1650.001884749636.8204370
CTGCCCC65650.035.83571269
Produced by FastQC (version 0.11.5)