FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685388

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685388
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406364
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT13650.3359057396816647No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC12610.31031292142020456No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA11560.2844740183677688No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG10430.25666643698752845No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT9590.23599531454557982No Hit
GTGTAGCAACTGGCGGGGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA9570.23550314496362865No Hit
GTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCTGCTTAGCCTGGTA9490.23353446663582403No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT9240.22738234686143455No Hit
GTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAAAAACA8330.20498863088265692No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAG7700.1894852890511955No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7630.18776269551436645No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7600.1870244411414397No Hit
GTTTATTACTGTCAGCAGTGTAGCAACTGGCGGGGGCTCACTTTCGGCGG7440.18308708448583044No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTG7310.17988798220314792No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG7260.17865755824827No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATATATGACGAGCC7170.17644279512948982No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7160.17619671033851425No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCTGCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC7120.17521237117461191No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATATA7080.1742280320107096No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC7070.17398194721973403No Hit
TTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC7000.172259353682905No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAAC6950.1710289297280271No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT6820.16782982744534455No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6790.16709157307241781No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6730.16561506432656437No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC6710.1651228947446132No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG6670.1641385555807109No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6620.162908131625833No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA6590.16216987725290627No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6500.15995511413412605No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC6490.15970902934315048No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGAATATGCCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGG6400.15749426622437027No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC6360.15650992706046796No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT6340.15601775747851682No Hit
CAGTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAAAAA6290.15478733352363888No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAAC6240.153556909568761No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA5930.14592828104851807No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5900.14519002667559136No Hit
GAGTTTGGACTGAGCTGGGTTGTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAG5780.1422370091838844No Hit
GAGCTGGGTTGTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCG5360.13190144796291012No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG5190.12771800651632526No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGC5110.12574932818852064No Hit
GTCAGAGTGTCAGCAGCTGCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATATATGA5090.12525715860656947No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCCCCCGCCAGTTGCTACAC5060.12451890423364274No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5020.12353456506974042No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT5010.12328848027876485No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATATATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT5000.12304239548778927No Hit
ATATATGACGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGCAGCTGCTGACACTCTGACTGGC4970.12230414111486254No Hit
GTATTACTGTGCAAAAAATATTGCCCCAGACCCCGGGTACTACTACATGGACGTCTGGGGCAAAGGGACCACGG4890.12033546278705791No Hit
CTATGCAGACTCTGTGAAGGGTCGATTCACCATCTCTAGAGACAACAGCAAAAACTCCCTGTTTTTGCAAATGAA4820.11861286925022886No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA4790.11787461487730214No Hit
CTCTTATTAGTTGGGATGGCGGTAGGACATACTATGCAGACTCTGTGAAG4780.11762853008632655No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT4750.11689027571339981No Hit
GCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATATATGATGCATCCAACAGGGCCAC4710.1159059365494975No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT4690.11541376696754635No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA4690.11541376696754635No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG4640.11418334301266844No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA4480.11024598635705918No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4430.1090155624021813No Hit
GCACTGAACACAGAACTCACCATGGAGTTTGGACTGAGCTGGGTTGTCCTTGTTGCTATTTTAAAAGGTGTCCAG4410.10852339282023014No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATATATGATGCATCCAACAGGGCCACTGGCATC4380.1077851384473034No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4320.10630862970144994No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC4320.10630862970144994No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4310.10606254491047436No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4300.10581646011949877No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA4230.10409386658266973No Hit
GTCATATATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAG4210.10360169700071857No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC4200.103355612209743No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG4150.1021251882548651No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC4130.10163301867291395No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC4120.10138693388193835No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC4120.10138693388193835No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC4090.10064867950901162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGTA651.5128535E-892.1449670
GTTATTA601.01521604E-466.5491370
TAGGCAA500.004119546659.89421570
TAAAACC200.00779331650.9988944
CTAACCC200.00779331650.99889418
TGTTAGA3350.047.6769970
GAGTAAT306.7571737E-445.3602561
TCGACGT1900.041.2432729
ACTCGAC1900.041.2178627
CTTATAC1900.041.1823351
TAATCGT504.045267E-641.0821938
TATACAC2000.040.7991143
GTGGCCG6000.039.92947870
TTATACA2050.039.8040122
ATACACA2250.039.2880364
CCGGGCA350.00138519239.2036241
GTCGTAT350.001392817739.15986639
TACACAT2000.039.099155
GTTTACG350.001447135138.85639
TAGTTCG350.001447135138.85637
Produced by FastQC (version 0.11.5)