FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences406364
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25600.6299770648974811No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT13550.33344489177190895No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA11120.2736462875648433No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA10270.25272908033191915No Hit
GTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCTGCTTAGCCTGGTA9230.227136262070459No Hit
GTGTAGCAACTGGCGGGGGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCAT9020.22196848145997183No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTCAGCA8320.20474254609168135No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT8250.2030199525548523No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC8170.20105127422704766No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT7850.19317656091582916No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC7830.192684391333878No Hit
TTGCTACACTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTC7480.18407142364973275No Hit
CTCCTGCAGGGCCAGTCAGAGTGTCAGCAGCTGCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT7300.17964189741217232No Hit
GTTTATTACTGTCAGCAGTGTAGCAACTGGCGGGGGCTCACTTTCGGCGG7220.1776732190843677No Hit
GTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAAAAACAGGGAGTTTTTGCTGTTGTCTCTAGA7060.17373586242875846No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7010.17250543847388056No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATATATGACGAGCCTG6900.1697985057731492No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCA6880.16930633619119806No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT6530.1606933685070528No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC6490.15970902934315048No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA6470.15921685976119931No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC6370.15675601185144356No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTGATGAATATGCCATGCACTGGGTCCGTCAAGCTCCGGGGAAGGGT6280.1545412487326633No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT6260.15404907915071217No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAACTG6250.15380299435973657No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTGTAGCAACTGGCGGGGGCTCACTTTCGGCGGAG6020.1481430441672983No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC5970.1469126202124204No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCCCCCGCCA5740.14125267001998207No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT5730.1410065852290065No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA5710.14051441564705533No Hit
CAGTAATACAAGGCGGTGTCCTCAGCTCTCAGACTGTTCATTTGCAAAAACAGGGAGTTTTTGCTGTTGTCTCTA5680.13977616127412862No Hit
GAGTTTGGACTGAGCTGGGTTGTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGT5560.13682314378242166No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG5510.13559271982754378No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA5500.13534663503656819No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5380.13239361754486126No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5360.13190144796291012No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG5310.1306710240080322No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG5300.13042493921705664No Hit
GAGCTGGGTTGTCCTTGTTGCTATTTTAAAAGGTGTCCAGTGTGAAGTGCAGCTGGTGGAGTCTGGGGGAGTCGT5290.13017885442608107No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA5080.1250110738155939No Hit
CTCTTATTAGTTGGGATGGCGGTAGGACATACTATGCAGACTCTGTGAAG4920.12107371715998465No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCCGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCA4770.11738244529535097No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4690.11541376696754635No Hit
CTATGCAGACTCTGTGAAGGGTCGATTCACCATCTCTAGAGACAACAGCAAAAACTCCCTGTTTTTGCAAATGAA4680.11516768217657077No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATATATGATGCATCCAACAGGGCCACTGGCATC4610.1134450886397417No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC4610.1134450886397417No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG4590.11295291905779055No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA4590.11295291905779055No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA4580.11270683426681496No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA4570.1124607494758394No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATATATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT4520.1112303255209615No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA4460.10975381677510802No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT4440.10926164719315687No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC4430.1090155624021813No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4360.10729296886535225No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4350.10704688407437667No Hit
GTATTACTGTGCAAAAAATATTGCCCCAGACCCCGGGTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGT4200.103355612209743No Hit
GTCAGAGTGTCAGCAGCTGCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATATATGAT4190.10310952741876742No Hit
ATATATGACGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGCAGCTGCTGACACTCTGACTGGC4140.10187910346388952No Hit
GCTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCGTCATATATGATGCATCCAACAGGGCCACTG4130.10163301867291395No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCC4120.10138693388193835No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT4080.10040259471803603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAAAA600.001502056577.2276270
CGGCCAA200.00681015777.22761570
CTATAAC150.002475330468.1341634
CAACCCA1102.3446357E-456.16554370
CTAATGG252.7302888E-454.50733633
TCTAATG252.7352868E-454.4871632
TCGTAGC200.00750819951.4818845
GGCCATT200.0078269150.943436
GAGCCAT5650.046.47325570
TAATTGA306.4107834E-445.84718747
TAGAACA306.814127E-445.283055
CTGTGTT1407.704699E-444.13006670
GGTATCA3350.043.6309621
CTTCCAT1856.0153674E-541.74465670
GCATATA350.00116136740.63399565
ACCTATG350.001326743939.54886253
GACCTAT350.001334943239.4993452
TGACCTA350.001343182639.4499451
GACATAT350.001396023639.14154441
TTTTCTA350.00144261638.8810229
Produced by FastQC (version 0.11.5)