FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094951
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGG81510.7444168734491315No Hit
GCCTTGGACTGACCCAAGACGGTCAGCCTGGTCCCTCCGCCGAATGACACGGCGTAGTGGCCTCCATATGAGC48300.4411156298318372No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAG28200.25754577145461305No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28100.2566324885771144No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT25950.2369969067108939No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22530.20576263230044084No Hit
TCTCTGGGCTCCAGGCTGAGGACGAAGGTGATTATTTCTGCAGCTCATAT22060.2014702027761973No Hit
GTAATACACGGCAGTGTCTTCAGTCTCCAGGGTACTGATCTCCAGATAAG21920.2001916067476992No Hit
GTCTTGGGTCAGTCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC20860.19051080824621375No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCC20170.18420915639147323No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20070.18329587351397458No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19770.18055602488147873No Hit
GACCCAAGACGGTCAGCCTGGTCCCTCCGCCGAATGACACGGCGTAGTGGCCTCCATATGAGCTGCAGAAATAA19140.17480234275323736No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18760.17133186781874257No Hit
GTGTATTACTGCGCGAGAGCCCCTCTGGATTTTTGGGGTGATTATACTTCATCTCTCCGCTTTGACAATTGGGGC18580.16968795863924505No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18450.1685006908984968No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC18430.1683180343229971No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGC17490.15973317527451No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGG17150.15662801349101466No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG17140.1565366852032648No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT17070.15589738718901577No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT16820.1536141799952692No Hit
GCGTAGTGGCCTCCATATGAGCTGCAGAAATAATCACCTTCGTCCTCAGC16530.15096565965052317No Hit
CTCCTGCACTGGACTCAGCAGTGGCTTTGTTGGTTCTGATTATGTCTCCTGGTACCAACAGATTTCAGGCAGAGG16440.1501437050607744No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16150.1474951847160284No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGACTGACCCAAGACGGTCAGCCTGGTCCCTC16140.14740385642827852No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15760.14393338149378374No Hit
GGACTCAGCAGTGGCTTTGTTGGTTCTGATTATGTCTCCTGGTACCAACA15480.1413761894367876No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGCCTGAGCAG15170.13854501251654183No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15150.1383623559410421No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15050.13744907306354348No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGACTCAGCAGTGGCTTT14710.13434391128004816No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCT14680.13406992641679855No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG14290.13050812319455393No Hit
GTATTACTGCGCGAGAGCCCCTCTGGATTTTTGGGGTGATTATACTTCAT14240.1300514817558046No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14150.12922952716605585No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC14140.12913819887830597No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14120.12895554230280623No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC14100.12877288572730652No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13900.12694631997230926No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13850.12648967853355994No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG13200.12055333982981886No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13050.11918341551357094No Hit
CCACTACGCCGTGTCATTCGGCGGAGGGACCAGGCTGACCGTCTTGGGTCAGTCCAAGGCTGCCCCCTCGGTCA13040.11909208722582106No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12880.11763083462182325No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC12700.11598692544232572No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12600.1150736425648271No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT12580.11489098598932738No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA12330.11260777879558082No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT12320.11251645050783093No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA12150.11096386961608327No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12130.11078121304058355No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC12000.10959394529983535No Hit
GTCGATCACCATCTCCTGCACTGGACTCAGCAGTGGCTTTGTTGGTTCTGATTATGTCTCCTGGTACCAACAGAT11990.10950261701208547No Hit
CCATATGAGCTGCAGAAATAATCACCTTCGTCCTCAGCCTGGAGCCCAGA11980.1094112887243356No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11820.10795003612033781No Hit
GGGCTTCACAGGGCGGTTTGTCTTCTCCTTGGACAATTCTGTCGGCACGG11640.10630612694084028No Hit
GCACTGGACTCAGCAGTGGCTTTGTTGGTTCTGATTATGTCTCCTGGTAC11440.10447956118584303No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC11070.1011004145390981No Hit
GTCCAGTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGG11060.10100908625134822No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG10980.10027845994934934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG4000.078.8423270
ATTACAA600.00691593252.5615570
CAAGGAG11850.051.45265670
GTCTATA200.00797959950.7008131
GATTTAG854.4056607E-449.46969270
GCTTCGT306.981483E-445.067391
CGTATGC3400.043.418141
TCGTATG3350.042.9914740
GGTATCA10200.040.42811
GTATCAA26750.039.9288671
TAGGCAT3750.039.76647629
ATGCCGT3750.039.5160844
GCCGTCT3800.039.1181946
GACTAGG3750.038.80049526
TATACAC3700.038.3576743
ACTAGGC3950.037.71670527
TCTCGTA3450.037.65268338
GTGGTAT2800.037.422031
CATTCGG16600.037.3630370
TTATACA3800.037.3482632
Produced by FastQC (version 0.11.5)