FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1094951
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT87860.8024103361702943No Hit
GCCTTGGACTGACCCAAGACGGTCAGCCTGGTCCCTCCGCCGAATGACACGGCGTAGTGGCCTCCATATGAGCT42990.3926203090366601No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35600.32512870438951147No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27510.2512441195998725No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG26160.23891480075364102No Hit
GTAATACACGGCAGTGTCTTCAGTCTCCAGGGTACTGATCTCCAGATAAGCCGTGCCGACAGAATTGTCCAAGGA24440.22320633526066463No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21960.20055691989869867No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA21450.19589917722345565No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21020.19197206085021157No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT20040.18302188865072502No Hit
TCTCTGGGCTCCAGGCTGAGGACGAAGGTGATTATTTCTGCAGCTCATATGGAGGCCACTACGCCGTGTCATTCG19890.18165196433447706No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19580.17882078741423132No Hit
GTCTTGGGTCAGTCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG18900.17261046384724066No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA18690.17069256980449354No Hit
GACCCAAGACGGTCAGCCTGGTCCCTCCGCCGAATGACACGGCGTAGTGGCCTCCATATGAGCTGCAGAAATAAT17550.16028114500100918No Hit
GTGTATTACTGCGCGAGAGCCCCTCTGGATTTTTGGGGTGATTATACTTCATCTCTCCGCTTTGACAATTGGGGC17340.15836325095826206No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA17100.15617137205226533No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17050.15571473061351604No Hit
CTCCTGCACTGGACTCAGCAGTGGCTTTGTTGGTTCTGATTATGTCTCCTGGTACCAACAGATTTCAGGCAGAG17000.15525808917476672No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG16440.1501437050607744No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16220.14813448273027743No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGACTGACCCAAGACGGTCAGCCTGGTCCCTC15380.14046290655928895No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTG14950.13653579018604484No Hit
GCGTAGTGGCCTCCATATGAGCTGCAGAAATAATCACCTTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAG14850.13562250730854625No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC14830.1354398507330465No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC14530.1327000021005506No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14470.13215203237405146No Hit
GGACTCAGCAGTGGCTTTGTTGGTTCTGATTATGTCTCCTGGTACCAACA14450.1319693757985517No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTG14390.13142140607205255No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG14350.13105609292105308No Hit
CCACTACGCCGTGTCATTCGGCGGAGGGACCAGGCTGACCGTCTTGGGTCAGTCCAAGGCTGCCCCCTCGGTCA14010.12795093113755776No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGACTCAGCAGTGGCTTT13990.12776827456205803No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTT13980.12767694627430815No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13760.12566772394381118No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC13760.12566772394381118No Hit
GTATTACTGCGCGAGAGCCCCTCTGGATTTTTGGGGTGATTATACTTCATCTCTCCGCTTTGACAATTGGGGCCA13690.12502842592956215No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCT13670.12484576935406241No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13500.12329318846231475No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13160.12018802667881942No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA13020.11890943065032133No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTT12820.11708286489532409No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC12740.11635223859332519No Hit
GCTCCACACCATGGACTGGACCTGGAGGATCCTCTTCTTGGTGGCAGCAG12370.11297309194658026No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACG12350.11279043537108054No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTG12330.11260777879558082No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG12190.11132918276708273No Hit
GTCGATCACCATCTCCTGCACTGGACTCAGCAGTGGCTTTGTTGGTTCTG12060.11014191502633451No Hit
GGGCTTCACAGGGCGGTTTGTCTTCTCCTTGGACAATTCTGTCGGCACGGCTTATCTGGAGATCAGTACCCTGGA12050.11005058673858464No Hit
CCATATGAGCTGCAGAAATAATCACCTTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGGCCGTGTTGCCA11720.10703675324283918No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGG11620.10612347036534056No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11610.1060321420775907No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGC11290.10310963686959507No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG11180.10210502570434658No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)