FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937316
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC45630.4868155456644291No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT42630.45480926389819437No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC32170.3432140281399229No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT29530.3150485001856364No Hit
GTTTATTACTGTCAACAGCGTTTCAGCTGGCCTCCGGCCACCTTCGGCCA28910.3084338686206146No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27880.2974450452142074No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCG26710.2849625953253759No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26620.2840024068723888No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG26600.2837890316606139No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25960.2769610248838172No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTTTCAGC25170.26853270401870877No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24600.26245151048312415No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT24350.25978432033593796No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAG24080.2569037549769768No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT23630.2521028127120416No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG23310.24868880932364326No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGAGGCATCGTAGATGAGGAGCC22950.24484805551169508No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA22720.24239424057628375No Hit
CTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGC22530.24036717606442226No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC22520.24026048845853482No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTA22440.2394069876114352No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTAATAT21870.23332579407585063No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC21460.22895160223446523No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTTTCAGC21320.22745797575204094No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGAGGCATCGTAGA20310.2166825275574086No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19880.2120949605042483No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19650.20964114556883698No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAACTTCCAGTCGTGTCC17840.19033068890320873No Hit
GGACACGACTGGAAGTTAAACGAACTGTGGCTGCACCATCTGTCTTCATC17210.18360936973229944No Hit
GCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTC17190.18339599452052457No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17110.18254249367342498No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17070.18211574324987517No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16340.1743275480200914No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA15950.1701667313904809No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA15930.169953356178706No Hit
GTGCAGCCACAGTTCGTTTAACTTCCAGTCGTGTCCCTTGGCCGAAGGTGGCCGGAGGCCAGCTGAAACGCTGT15910.1697399809669311No Hit
CCTTCGGCCAAGGGACACGACTGGAAGTTAAACGAACTGTGGCTGCACCA15890.1695266057551562No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15890.1695266057551562No Hit
GTTGGAGGCATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTT15750.16803297927273192No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGATGCCTCCAACAGGGCCACTGGCATCCCAGCCAGGT15500.1653657891255457No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15430.16461897588433355No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14840.15832440713697407No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC14830.15821771953108663No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG14440.15405690290147614No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA14430.15395021529558867No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13960.1489358978188786No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGATGCCTCCAACAGGGCCACTGGCATC13920.14850914739532878No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13630.14541520682459277No Hit
CTGCTACTCTGGCTCCCAGATTCCACCGGAGAAATTGTGTTGACACAGTC13510.1441349555539434No Hit
CTCCCAGGCTCCTCATCTACGATGCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTC13170.14050757695377014No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC13010.13880057525957093No Hit
CCTCATCTACGATGCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACT12980.1384805124419086No Hit
GTCGTGTCCCTTGGCCGAAGGTGGCCGGAGGCCAGCTGAAACGCTGTTGA12810.13666682314182196No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12750.13602669750649726No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT12570.1341063206005232No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAG12550.1338929453887483No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC12240.13058562960623737No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC12210.13026556678857504No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA12100.1290920031238131No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA12010.12813181467082607No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11880.12674487579428922No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA11770.1255713121295273No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11610.1238643104353281No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA11530.1230108095882285No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAAATATTA11510.12279743437645362No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11260.12013024422926741No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT11250.12002355662337995No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAACTTCCAGTCG11240.1199168690174925No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGG11180.1192767433821678No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT11180.1192767433821678No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT11150.11895668056450545No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGC11100.11842324253506821No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT11080.1182098673232933No Hit
CATCTACGATGCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCA11030.11767642929385608No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG10950.11682292844675647No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG10930.1166095532349816No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA10880.11607611520554435No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG10750.11468917632900752No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG10730.11447580111723261No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG10570.11276879942303342No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC10440.11138186054649658No Hit
CACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTTTCAGCTGGCCTCCGGCCAC10420.1111684853347217No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC10380.1107417349111719No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT10340.1103149844876221No Hit
GGCCGAAGGTGGCCGGAGGCCAGCTGAAACGCTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGC10320.11010160927584721No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTACCAACAGAAAC10310.10999492166995975No Hit
CTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCACT10220.10903473321697273No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10220.10903473321697273No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTG10100.10775448194632334No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT10090.10764779434043589No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC10090.10764779434043589No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGT10020.10690098109922375No Hit
GGTTTCTGTTGGTACCAGGCTAAGTAAATATTAACATTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC9820.10476722898147477No Hit
ATAATACACAGCCGTGTCCTCGGCTGTCAGGCTGTCCATTTGAAGAAACA9820.10476722898147477No Hit
GCCACAGTTCGTTTAACTTCCAGTCGTGTCCCTTGGCCGAAGGTGGCCGGAGGCCAGCTGAAACGCTGTTGACAG9810.10466054137558732No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC9700.10348697771082538No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCT9640.10284685207550069No Hit
ACCCTCTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTA9640.10284685207550069No Hit
CCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAAC9570.10210003883428855No Hit
GCTGAAACGCTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAG9510.10145991319896386No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)