FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences937316
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA46090.4917231755352517No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT43570.46483789885161464No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32450.3462012811047715No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC31530.33638602136312623No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT30720.32774432528624287No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC29340.31302143567377494No Hit
GTTTATTACTGTCAACAGCGTTTCAGCTGGCCTCCGGCCACCTTCGGCCA28190.3007523609967183No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA25830.2755740860072804No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT25770.27493396037195567No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC25480.2718400198012197No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG24830.26490532541853545No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24210.25829069385351366No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT23920.25519675328277763No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC23910.2550900656768902No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCA23380.24943562256485538No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTTTCAGCTGGCCTCCGGCCACCTTCGGCCAAG23370.24932893495896793No Hit
CTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT23330.24890218453541813No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAGT23130.2467684324176692No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA22810.2433544290292708No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTA22680.241967490152734No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT22010.23481942055827493No Hit
GTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGAGGCATCGTAGATGAGGAGCCTG21320.22745797575204094No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTTTCAGCTG19520.20825420669230016No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19460.20761408105697543No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGAGGCATCGTA19280.20569370415100138No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT18990.20259976358026535No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18370.19598513201524353No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAACTTCCAGTCGTGTCCCTTGGCCGAAGGTGGCCGGAGGCCA18320.19545169398580628No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT17680.18862368720900954No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA17600.18777018636190998No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA17110.18254249367342498No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA16970.1810488671910007No Hit
GGACACGACTGGAAGTTAAACGAACTGTGGCTGCACCATCTGTCTTCATC16530.17635461253195295No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG16500.1760345497142906No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16200.17283392153766713No Hit
GCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTC16180.17262054632589224No Hit
GTTGGAGGCATCGTAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTT15630.16675272800208255No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA15280.16301866179602184No Hit
GTGCAGCCACAGTTCGTTTAACTTCCAGTCGTGTCCCTTGGCCGAAGGTGGCCGGAGGCCAGCTGAAACGCTGTT15230.16248522376658459No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGATGCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTT14960.15960465840762347No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT14320.15277665163082674No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14200.15149640036017736No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA14090.15032283669541543No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA13680.14594864485403003No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG13580.14488176879515555No Hit
GCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTACGATGCCTCCAACAGGGCCACTGGCATC13490.14392158034216848No Hit
CTGCTACTCTGGCTCCCAGATTCCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA13450.1434948299186187No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT13290.14178782822441952No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT13280.14168114061853207No Hit
CCTTCGGCCAAGGGACACGACTGGAAGTTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT13200.14082763977143248No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13180.1406142645596576No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC12630.1347464462358479No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC12580.13421300820641063No Hit
GTCGTGTCCCTTGGCCGAAGGTGGCCGGAGGCCAGCTGAAACGCTGTTGACAGTAATAAACTGCAAAATCTTCAG12570.1341063206005232No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA12530.13367957017697338No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA12410.13239931890632403No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC12400.13229263130043656No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA12390.1321859436945491No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12110.12919869072970055No Hit
CTCCCAGGCTCCTCATCTACGATGCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTG11930.12727831382372648No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC11880.12674487579428922No Hit
CCTCATCTACGATGCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTT11650.12429106085887791No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCA11610.1238643104353281No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11610.1238643104353281No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCAGTGAACCTGGCTGGGATGCCAGTG11420.12183724592346658No Hit
GCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGCTAAGTAAATATTAACATTCTGACTGGCCCTGCAGGAGA11360.12119712028814188No Hit
CTCTTCCTCCTGCTACTCTGGCTCCCAGATTCCACCGGAGAAATTGTGTT10750.11468917632900752No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTAACTTCCAGTCGT10700.11415573829957028No Hit
GGTTTCTGTTGGTACCAGGCTAAGTAAATATTAACATTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC10520.11223536139359618No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT10510.11212867378770873No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA10460.11159523575827149No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGT10400.1109551101229468No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTT10320.11010160927584721No Hit
GGCCGAAGGTGGCCGGAGGCCAGCTGAAACGCTGTTGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCT10310.10999492166995975No Hit
CTGCAGGAGAGGGTGGCTCTTTCCCCTGGAGACAAAGACAGGGTGGCTGGAGACTGTGTCAACACAATTTCTCCG10210.10892804561108527No Hit
CATCTACGATGCCTCCAACAGGGCCACTGGCATCCCAGCCAGGTTCACTGGCAGTGGGTCTGGGACAGACTTCA10180.10860798279342292No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT10140.10818123236987313No Hit
GGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTACCAACAGAAAC10000.10668760588744884No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT9980.10647423067567394No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9910.10572741743446182No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCA9900.10562072982857436No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGA9880.10540735461679945No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAG9770.10423379095203752No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG9670.10316691489316303No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCA9610.10252678925783834No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGA9560.1019933512284011No Hit
GGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGC9540.1017799760166262No Hit
CACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAACAGCGTTTCAGCTGGCCTCCGGCCAC9510.10145991319896386No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAATGTTAATATTTACTTAGCCTGGTACCAACAGAAACCTGGCCAG9500.1013532255930764No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTG9440.10071309995775171No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA507.2085456E-492.95061570
CTATTAA700.002740845566.393370
AATGGTA1902.5102054E-1065.228570
TATGAAT800.0046517458.09413570
GGTATCA5300.041.9249041
ATTAACA16400.040.61866470
GACAGGT2200.039.7882351
GGTTAGC350.001519705438.476541
ATACGTA350.001524896438.4498511
TATAGCC350.001526096338.4436952
AAGCGGT6950.035.66450570
AAGGACA26750.035.32702370
GAGCAAA25150.034.49459570
CTTCTAA4055.2550604E-934.4261570
TAATATT24800.034.35674770
CCGTTAA400.0029281133.66337616
TACCGCG400.00293885433.638238
ACAGGTA1750.032.7050551
ACAGCGT30350.032.6679870
TGGTATC1058.421921E-1032.0364072
Produced by FastQC (version 0.11.5)