FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1012555
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43860.433161655416249No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT32930.325216901797927No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA30740.3035884470473209No Hit
GTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGAGTGTTCCAGGAATTGTCTCTGGA28460.2810711516905255No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA26400.2607265778155261No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT22980.22695063478033292No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21370.21105026393627999No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC21230.20966762299331887No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA18950.18715032763652345No Hit
GGTTTACGTTGGTACCAGGCTAACTTGTTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC18120.17895324204611107No Hit
CTATATTACTGTGCGATTCGAGATTTTTACCATGGTTCAGGAAATTATTTCTTTGACTGCTGGGGCCAGGGAACC17990.1776693611705043No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC17700.1748053192172277No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT17560.17342267827426658No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17280.17065739638834435No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT17250.17036111618628125No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAAGTTAGCCTGGTA17050.1683859148391939No Hit
ACGTAAACCTGGCCAAGCTCCCAGGCTCCTCATCTATGATACATCCACCAGGGCCACTGGTATCCCACCCAGGTT17040.16828715477183953No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC17010.16799087456977646No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAACAAGTTAGCCTGGTACCAACGTAAACCTGGCCAAGCTCCCAGGCT16560.16354667153882999No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16290.1608801497202621No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA15880.1568309869587331No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAA15590.1539669450054565No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGT15470.1527818241972041No Hit
ATTATAGGTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA15120.1493252218398013No Hit
CCTATAATAATGCTGACAGTAATAAACTGCAAAATCTTCAGGCTGCAGGCTGCTGACGGTGAGAGTGAACT15100.14912770170509257No Hit
ATATTACTGTGCGATTCGAGATTTTTACCATGGTTCAGGAAATTATTTCTTTGACTGCTGGGGCCAGGGAACC15030.148436381233612No Hit
CTGGTATCCCACCCAGGTTCAGTGGCAGTGGGTCAGGGACAGAGTTCACTCTCACCGTCAGCAGCCTGCAGCCT14710.1452760590782723No Hit
GAGTTCACTCTCACCGTCAGCAGCCTGCAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCATTATTATAGGTG14660.14478225874150044No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14590.1440909382700199No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14420.14241201712499568No Hit
GTATTAGTGAGACTGGCAGTCGCACGTTTTATGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATT14420.14241201712499568No Hit
GAGTGAACTCTGTCCCTGACCCACTGCCACTGAACCTGGGTGGGATACCAGTGGCCCTGGTGGATGTATCATA14320.141424416451452No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC14170.13994301544113652No Hit
GTTTATTACTGTCAGCATTATTATAGGTGGCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA13730.13559757247754442No Hit
CTCCTGTGGCGCCCATGGATTCAGTTTTGAAAACTATGCCATGGGCTGGGTCCGCCAGGCTCCAGGGAAGGG13630.13460997180400078No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT13620.1345112117366464No Hit
GGATACCAGTGGCCCTGGTGGATGTATCATAGATGAGGAGCCTGGGAGCTTGGCCAGGTTTACGTTGGTACCAG13440.1327335305242678No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA13350.13184468991807852No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA13340.13174592985072414No Hit
GTAATAAACTGCAAAATCTTCAGGCTGCAGGCTGCTGACGGTGAGAGTGAACTCTGTCCCTGACCCACTGCCA13210.1304620489751174No Hit
GTCAGAGTGTTAGCAACAAGTTAGCCTGGTACCAACGTAAACCTGGCCAAGCTCCCAGGCTCCTCATCTATGATA13210.1304620489751174No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12970.1280918073586126No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTG12780.12621536607887968No Hit
GCCCTATATTACTGTGCGATTCGAGATTTTTACCATGGTTCAGGAAATTA12740.1258203258094622No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA12510.12354884426031179No Hit
GTCCCTGACCCACTGCCACTGAACCTGGGTGGGATACCAGTGGCCCTGGTGGATGTATCATAGATGAGGAGCCTG12250.12098108250909827No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGG12200.12048728217232643No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG12130.11979596170084589No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC11880.11732696001698674No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11870.11722819994963236No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11770.1162405992760887No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11500.11357407745752084No Hit
GTGTGAGGTGGAGCTGGTGGAGTCTGGGGGAGGCCTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGGCGC11480.11337655732281209No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11210.11071003550424423No Hit
CACTAATACCTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCCCATGGCATAGTTTTCAAAA10890.10754971334890451No Hit
CAGTAATATAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGAGTGTTCCAGGAATTGTCTCTG10840.10705591301213267No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCA10610.10478443146298226No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGCAGGGCACAGTCACATCCTGGCTGG10580.10448815126091915No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA10390.10261170998118621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)