FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685403

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685403
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1949254
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATAGAGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTCCAGATACAGTGAGTTCTTGGCGTTGTCTCTGGA95280.4888023828603148No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG61670.31637744490969366No Hit
CTATATTACTGTGCGAGAGTAGCAGTGGCTGGTACAAGGTACTACTACTACTACATGGACGTCTGGGGCAAAGG49580.2543537168578338No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGT49290.25286596821142854No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA45310.2324479005814532No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA44180.226650811028219No Hit
GCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATACCACGAGAGTGCTGGTGCTTGTATATGA40010.20525801152646092No Hit
CAGTAATATAGAGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTCCAGATACAGTGAGTTCTTGGCGTTGTCTCTG38160.1957672011959447No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT37260.19115005022434226No Hit
TACATATACTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACGCCAAGAACTCACTGTATCTG36470.18709721770482451No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCT36340.18643029589781526No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36220.18581467576826827No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC36000.18468603886409876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG35170.18042799963473205No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC34990.17950456944041157No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33580.17227103291823437No Hit
CTCCTGTGTAGCCTCTGGGTTCACCTTCAGTGGCTATAGCCTGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG32120.1647809880087459No Hit
GTATATGTAAGTACTACTAGTACTAATGGATGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCT31450.1613437756187752No Hit
GACCTAGGACGGTCACCGTGGTCCCTCCGCCGAATACCACGAGAGTGCTG30710.1575474514865687No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC30530.15662402129224823No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG29690.15231468038541923No Hit
ATATTACTGTGCGAGAGTAGCAGTGGCTGGTACAAGGTACTACTACTACTACATGGACGTCTGGGGCAAAGGGAC29550.15159645690094775No Hit
GATTATTACTGCAGCTCATATACAAGCACCAGCACTCTCGTGGTATTCGGCGGAGGGACCACGGTGACCGTCCTA28990.14872356296306175No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG28080.14405511031399704No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCACCGTGGTCCCTC27050.13877103753538533No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTCCAAGGACGTCATGCAGGGCACAGAC26630.13661636708197084No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTT26130.13405128320885837No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25760.13215312114275513No Hit
GGCCTGGACTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC25450.1305627691414254No Hit
GCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGGTTTTC25450.1305627691414254No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT25040.12845940036547315No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACACACATGGAGGACGCATTCTGCTGGAA24940.12794638359085064No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT24890.12768987520353942No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCTTGTATTTCCAGGAGAAAGTGATGGA24580.12609952320220966No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC24460.12548390307266266No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCA24060.12343183597417268No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT23860.1224058024249277No Hit
ACCTTGTACCAGCCACTGCTACTCTCGCACAGTAATATAGAGCCGTGTCCTCGGCTCTCAGGGTGTTCATTTCCA23760.12189278565030519No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23310.11958421016450395No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT23070.11835296990540997No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC23050.11825036655048547No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAG22470.115274869257675No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATA22210.11394102564365649No Hit
CCATGGAACTGGGGCTCCGCTGGGTTTTCCTTGTTGCTATTTTAGAAGGTGTCCAGTGTGAGGTGCAGCTGGTG22160.11368451725634525No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGA22130.1135306122239585No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCGGCAGGTGAACATGCTCTGGCTGAGC22120.11347931054649625No Hit
GGTCACATACTTCTCCGGGGACAAGGGCTGCCCCCTCTGCATCCACTGCACGAAGACGTCCGCGGGAGAGAAGCC21510.11034990822129903No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC21500.11029860654383676No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21410.10983689144667654No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGG21230.10891346125235604No Hit
CTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTACCCAGGCAAAGC21220.10886215957489377No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGTA20950.10747701428341304No Hit
GTCTGGGGGAGGCCTGGTCAAGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGGTTCACCTTCAGTG20840.1069126958313283No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC20830.10686139415386603No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20520.10527104215253631No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGG20020.10270595827942383No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG19610.10060258950347159No Hit
AGCCTGGGGGGTCCCTGAGACTCTCCTGTGTAGCCTCTGGGTTCACCTTCAGTGGCTATAGCCTGAGCTGGGTCC19590.10049998614854708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)