FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715378
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG49020.2857679182081151No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA46880.27329253377389706No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG42390.24711754493761726No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAATTATGTCTCCTGGT40630.23685741568330712No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT40610.23674082330541724No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC38500.22444032743803408No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAATATCACAGTGGTACTGGTTGTATATGAGCTGCAGTAATAAT37110.21633715717468685No Hit
GATTATTACTGCAGCTCATATACAACCAGTACCACTGTGATATTCGGCGGAGGGACCAAGTTGACCGTCCTACGT36230.2112070925475318No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA35140.2048528079525329No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC34880.20333710703996435No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC34420.20065548234849698No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCAC33970.19803215384597447No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT32530.18963750263790255No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC31070.18112625905194074No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAATTATGTCTCCTGGTATCAACAAC30300.17663745250318005No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC30060.1752383439685014No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAATATCACAGTGGTACTGGTTGTATATGAGCTG29800.17372264305593285No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAATTATGTCTCCTGGTATCAACAACACCCAGGCAAAGC29740.1733728659222632No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29740.1733728659222632No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC29020.16917554031822724No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC28580.1666105080046497No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG28330.16515310328102611No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG27140.1582158567965778No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG27120.1580992644186879No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26310.15337727311414742No Hit
GGTCAGTAGTTGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT26170.15256112646891823No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26020.15168668363474408No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC25890.1509288331784598No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG25620.1493548360769463No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG25390.14801402373121259No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT24490.14276736672616763No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAATAT24210.14113507343570922No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23970.13973596490103055No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG23930.1395027801452508No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23780.13862833731107663No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG23180.13513056597437997No Hit
GACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22890.1334399764949766No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC22750.1326238298497474No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC22650.13204086796029796No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA22580.13163279463768335No Hit
TATACAACCAGTACCACTGTGATATTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCC22210.12947583564672044No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG22150.12912605851305076No Hit
GCCCTGACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCAC22130.12900946613516087No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21990.12819331948993165No Hit
GGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC21930.127843542356262No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG21860.12743546903364739No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA21790.12702739571103278No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21710.12656102619947324No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC21170.12341303199644627No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21070.12283007010699683No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20760.12102288824970356No Hit
GTAGTTGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC20310.1183995597471811No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC19830.11560134267782378No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19560.11402734557631028No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA19460.11344438368686087No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA19450.11338608749791593No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT19340.11274482941952153No Hit
CTACTGACCTCATAAATCACGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGATACCAGGAGACATAATTATAAGC19310.1125699408526867No Hit
CAGTGGTACTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGG19040.1109959437511732No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC18710.10907216951599005No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18630.1086058000044305No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACAC18250.10639054482452265No Hit
AGTCTGCCCTGACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACC18170.1059241753129631No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18070.10534121342351366No Hit
GTACCACTGTGATATTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACT18000.10493314010089905No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG17980.10481654772300916No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCC17890.10429188202250467No Hit
ATAATAGACAGCCGTGTCCTCGCCTCTCAGACTGTTCATTTGTAAATAAAGCGTGTTCTTGGCGTTGTCTCTGG17880.10423358583355971No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAATATCACAGTGGT17690.10312595824360578No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCC17680.10306766205466084No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17660.10295106967677095No Hit
GGTATCAACAACACCCAGGCAAAGCCCCCAAACTCGTGATTTATGAGGTCAGTAGTTGGCCCTCAGGGGTTTCTA17590.10254299635415634No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC17530.10219321922048667No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG17290.10079411068580801No Hit
ACGTAGGACGGTCAACTTGGTCCCTCCGCCGAATATCACAGTGGTACTGGTTGTATATGAGCTGCAGTAATAATC17200.10026944498530355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)