FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1715378
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGA46610.27171853667238355No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG46020.2682790615246319No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39810.2320771281898217No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAATTATGTCTCCTGGT39410.22974528063202396No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG38680.22548965883904307No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37170.2166869343083565No Hit
GATTATTACTGCAGCTCATATACAACCAGTACCACTGTGATATTCGGCGGAGGGACCAAGTTGACCGTCCTACGT35520.20706806313244078No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCAC34320.20007252045904753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33390.19465097488716773No Hit
GACGTAGGACGGTCAACTTGGTCCCTCCGCCGAATATCACAGTGGTACTGGTTGTATATGAGCTGCAGTAATAAT33020.1924940158962048No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC32920.19191105400675534No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC32830.19138638830625088No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA32100.18713076651326996No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTC31660.18456573419969244No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTC31240.18211729426400478No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGT30230.17622937918056544No Hit
GCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAATATCACAGTGGTACTGGTTGTATATGAGCTG29600.17255671927703398No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAATTATGTCTCCTGGTATCAACAAC29290.17074953741974072No Hit
CTCCTGCACTGGAACCAGCAGTGACATTGGTGCTTATAATTATGTCTCCTGGTATCAACAACACCCAGGCAAAGC29270.17063294504185084No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACGTAGGACGGTCAACTTGGTCCCTCCGCCGAATAT27700.16148044337749465No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27510.1603728157875407No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG26150.15244453409102834No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGC26080.15203646076841373No Hit
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25990.15151179506790924No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25380.14795572754226766No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCC25370.1478974313533227No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC25080.14620684187391933No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC25020.14585706474024968No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG24390.1421844048367182No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24310.14171803532515864No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24040.14014403822364516No Hit
GGTCAGTAGTTGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGCAACACGGCCTCCCTGACAAT23660.1379287830437373No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG23030.13425612314020582No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT22790.13285701460552718No Hit
GTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC22650.13204086796029796No Hit
GGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCC22540.13139960988190358No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC22300.13000050134722493No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAGG22260.12976731659144514No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAG22110.128892873757271No Hit
GACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22000.1282516156788766No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTG21940.12790183854520693No Hit
TATACAACCAGTACCACTGTGATATTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCC21850.12737717284470243No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC21670.12632784144369347No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCCCGTC21050.12271347772910693No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20980.12230540440649233No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTACCTGAGCCTGACGCCTGAGCAGTGGA20510.11956548352607997No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA20370.11874933688085076No Hit
GTAGTTGGCCCTCAGGGGTTTCTAATCGCTTCTCTGGCTCCAAGTCTGGC20270.11816637499140131No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC20220.1178748940466766No Hit
GCCCTGACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCAC20190.11770000547984176No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATG20100.11717533977933726No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAA19760.11519326935520917No Hit
GTGGTACTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGC19090.11128742469589793No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA18910.11023809329488894No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18450.10755646860342151No Hit
CTACTGACCTCATAAATCACGAGTTTGGGGGCTTTGCCTGGGTGTTGTTGATACCAGGAGACATAATTATAAGC18360.10703180290291703No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18350.10697350671397209No Hit
GGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCC18240.1063322486355777No Hit
GTACCACTGTGATATTCGGCGGAGGGACCAAGTTGACCGTCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACT18110.10557439817929343No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT17940.10458336296722938No Hit
AGTCTGCCCTGACTCAGCCTGCCTTCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACC17730.10335914299938556No Hit
GGTATCAACAACACCCAGGCAAAGCCCCCAAACTCGTGATTTATGAGGTCAGTAGTTGGCCCTCAGGGGTTTCTA17650.10289277348782601No Hit
CAGTGGTACTGGTTGTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGG17370.10126048019736757No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17290.10079411068580801No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTAGT650.004923298557.27916370
GTCTTAT700.00660082553.18779870
AAAAGGT13100.042.6314470
GTGGTAT3050.041.2389141
TCTCGCG12400.035.029670
TTTAAGT3001.0202166E-733.0946370
AATCCAT1952.9888068E-431.8217670
ATCAACG35700.030.9209753
GTGGCCG13250.030.90913470
CAACGCA36000.030.381145
TCAACGC35900.030.3702664
AACGCAG36400.030.1405986
GAGTTTA5550.030.012931
TATGGTT2155.2959454E-428.86159570
CGCAGAG38050.028.833588
ACGCAGA38400.028.5707747
AGAGTAC39600.027.96883611
GCACCGT16000.027.92359470
CAGAGTA39650.027.7565310
CTATAAT8050.027.75015470
Produced by FastQC (version 0.11.5)