FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426940
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT19010.4452616292687497No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA18210.4265236332974188No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13870.3248700051529489No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC12430.29114161240455333No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12070.28270951421745444No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT11820.27685389047641357No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG10860.2543682953108165No Hit
ATTTAATAGTTACCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCAT10630.24898112146905887No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC10320.24172014803016817No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA10170.23820677378554364No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT10170.23820677378554364No Hit
CTATTAAATTGTTGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTC10150.23773832388626037No Hit
GTGCGGTCCCATCAAAATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGCCTGCAGCCT10050.23539607438984403No Hit
GTCTCCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTT9460.22157680236098748No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9310.21806342811636295No Hit
GTCCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG9290.21759497821707968No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA9100.21314470417388862No Hit
CCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCC8920.20892865508033917No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8850.20728908043284772No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC8560.20049655689324025No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA8530.19979388204431534No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT8310.19464093315219938No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC8040.18831685951187518No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC7990.18714573476366703No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT7930.1857403850658172No Hit
GTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGGGACTGGAGTGGATT7910.18527193516653395No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG7770.18199278587155104No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT7660.17941631142549305No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA7550.17683983697943506No Hit
GAATTCACTCTCACAATCAGCAGCCTGCAGCCTGAGGATTTTGCAACTTATTACTGTCAACAATTTAATAGTTAC7360.17238956293624397No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACAT6990.16372323979950346No Hit
GTCCAAGGGTAACTATTAAATTGTTGACAGTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGA6970.16325478990022016No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT6810.15950719070595398No Hit
GGGCATTAGCAGTTCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTTTGGTGCATC6790.15903874080667074No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA6590.154354241813838No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGT6470.1515435424181384No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6270.14685904342530565No Hit
CACCAGGGAAAGCCCCTAACCTCCTGATCTTTGGTGCATCGACTTTGCAAAGTGCGGTCCCATCAAAATTCAGCG6260.14662481847566403No Hit
GTAATAAGTTGCAAAATCCTCAGGCTGCAGGCTGCTGATTGTGAGAGTGAATTCTGTCCCAGATCCACTGCC6020.14100341968426477No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6010.14076919473462313No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG5870.13749004543964022No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC5810.13608469574179044No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTTGACCCAGTCTCCATCCTTC5640.13210287159788262No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA5590.13093174684967443No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG5560.1302290720007495No Hit
ACTTATTACTGTCAACAATTTAATAGTTACCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA5530.12952639715182462No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC5450.12765259755469152No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT5410.12671569775612498No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA5390.12624724785684172No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT5290.12390499836042534No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA5230.12249964866257554No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGGTAA5190.12156274886400899No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT5120.11992317421651755No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC5090.11922049936759263No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC5080.118986274417951No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTA5050.11828359956902608No Hit
ATAATAGACGGCCGTGTCCGCAGCGGTCACAGAGGTCAGCTTCAGGGAGAACTGGTTCTTGGACGTGTCTACT4990.11687824987117627No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4890.11453600037475992No Hit
GCCCCTAACCTCCTGATCTTTGGTGCATCGACTTTGCAAAGTGCGGTCCCATCAAAATTCAGCGGCAGTG4860.11383332552583501No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC4760.11149107602941866No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCA4740.11102262613013537No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA4710.11031995128121047No Hit
TCTTTAGCCTGGTATCAGCAAACACCAGGGAAAGCCCCTAACCTCCTGATCTTTGGTGCATCGACTTTGCAAAGT4690.10985150138192722No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC4680.10961727643228557No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC4660.1091488265330023No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGG4650.10891460158336067No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA4640.10868037663371902No Hit
ATCTATTACAGTGGGAGCACCAAGTACAACCCCTCCCTCAAAGGTCGAGTCACCATGTCAGTAGACACGTCCAA4630.10844615168407738No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4590.10750925188551086No Hit
ACTTTGCAAAGTGCGGTCCCATCAAAATTCAGCGGCAGTGGATCTGGGACAGAATTCACTCTCACAATCAGCAGC4580.10727502693586921No Hit
GTACAACCCCTCCCTCAAAGGTCGAGTCACCATGTCAGTAGACACGTCCAAGAACCAGTTCTCCCTGAAGCTGAC4580.10727502693586921No Hit
GGACCGCACTTTGCAAAGTCGATGCACCAAAGATCAGGAGGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAG4540.10633812713730266No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT4530.10610390218766103No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGAT4520.10586967723801939No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG4500.10540122733873611No Hit
GGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCATCTGTCTTCATC4490.10516700238909447No Hit
GGTTAGGGGCTTTCCCTGGTGTTTGCTGATACCAGGCTAAAGAACTGCTAATGCCCTGACTGGCCCGGCAAGT4470.10469855248981122No Hit
CCATCCTTCCTGTCTGCATCTATAGGAGACAGAATCACCATCACTTGCCG4450.10423010259052795No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTG4440.1039958776408863No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGTAATTACTACTGGAGCTGGATCCGGCAGCCCCCAGGGAAGG4420.10352742774160303No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG4400.10305897784231977No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAGATGTGACATCCAGTT4330.10141940319482831No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT4270.10001405349697849No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTTA200.0056638853156.2393670
GAGGGTT454.576078E-4104.1595870
CAACTTT850.00570762955.143370
TCAACCG200.007750674651.0700337
GGTAATG200.008235428550.2923472
TATTCGT200.008235428550.2923472
GTTGACA8100.050.15090670
ACTGGTA350.00972868348.2099569
GCACGGA307.185177E-444.79876724
GAGCAAA12200.042.26146370
ACCAGGT1500.001022723341.66382670
GTATCAA10500.038.3629151
TAGGCAA1650.001634047137.87620570
GATTCAC802.1391315E-937.7634931
CGTCACA5000.037.49744470
GAGCCAT4701.4551915E-1136.56665870
TAGCAGT10250.035.0585970
GTGGTAT1450.034.725051
AAGCGGT3606.594564E-834.7198670
TATCAAC11900.033.809982
Produced by FastQC (version 0.11.5)