FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685412

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685412
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911949
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT35000.38379339195503254No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31830.34903267616939104No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25400.27852434730450937No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22740.24935604951592688No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT22180.24321535524464635No Hit
GTCTGGGACAGACTTCACTCTCGCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCG20640.22632844599862492No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGCAGGGCCAGTCAGAGTATTAGCAA19900.21821395714014707No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGCAGGGCCAGTCAGAGTATTAGCAACTACTTAGCCTGGTA19820.21733671510139274No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19660.21558223102388402No Hit
CTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCAAGAACACGGTGTATCTGCAAATGAA18990.20823532894931623No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG18240.20001118483599412No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC18140.19891463228755119No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCC18090.1983663560133297No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18020.1975987692294196No Hit
GAGTATTAGCAACTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC17960.19694083770035384No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17860.1958442851519109No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC17830.195515319387378No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17760.19474773260346795No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC16590.18191806778668543No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA16480.1807118599833982No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC16370.17950565218011094No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTG16250.17818978912197941No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15770.17292633688945325No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT15760.17281668163460898No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15640.17150081857647742No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA14900.16338632971799957No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA14280.15658770391765328No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13720.15044700964637278No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT13680.1500083886269956No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACA13590.14902149133339693No Hit
GACTTCACTCTCGCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAAC13540.14847321505917546No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA13330.14617045470744527No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG13270.14551252317837948No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC12320.13509527396817148No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG12080.1324635478519084No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11900.13048975326471107No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC11810.1295028559711124No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCAC11770.12906423495173525No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGC11610.12730975087422652No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT11450.1255552667967178No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11310.12402009322889766No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11280.1236911274643648No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA11060.12127871185779031No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGT11030.12094974609325741No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10910.11963388303512586No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10830.11875664099637151No Hit
GCGTAGCAACTGGCCTACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCT10800.11842767523183861No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC10750.11787939895761715No Hit
GTATTAGCAACTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCC10690.11722146742855137No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA10690.11722146742855137No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTC10630.1165635358994856No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGCGACAGTCGGGGG10540.11557663860588695No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10440.11448008605744402No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT10360.11360284401868964No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10290.11283525723477957No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10100.11075180739273796No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCATGCGACGACCACGTTCCCATCTTGGGGGG9940.10899732331522925No Hit
CAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA9890.10844904704100777No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA9830.10779111551194201No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG9820.10768146025709772No Hit
GTCTGCGTAGTGTGTGATATCACCACTACCACTAATACCTGAGACCCACT9760.10702352872803195No Hit
ACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCA9720.10658490770865477No Hit
GTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGATTCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC9680.10614628668927759No Hit
GTCGTGTCCCTTGGCCGAAGGTAGGCCAGTTGCTACGCTGCTGACAGTAA9550.10472076837630175No Hit
GGCCTACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC9550.10472076837630175No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9400.10307593955363732No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC9400.10307593955363732No Hit
CTTCTGCACACCTCCCGCACAGAAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACACCG9390.10296628429879304No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC9260.10154076598581718No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9260.10154076598581718No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC9180.10066352394706284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG200.001198314481.8071669
TACGCGA150.002333163769.16508552
CTCGCTT150.00241994768.5310340
GCGTATG150.00241994768.5310340
TGTTAGA8350.053.17279470
CGCGTAT200.00757615451.36990739
GGTATCA6650.047.5995031
AATGGTA2400.046.24925270
AATCGCG306.585231E-445.60424836
CCGTGCG508.1861165E-443.6304869
GGCGGCG12550.041.81026570
CGTATGC1700.040.33910441
CTCTTAT955.456968E-1239.4103431
ATGCCGT1750.039.25380744
ATCGCGT350.001403472739.10444337
GTATCAA16400.038.602041
GCGTAGA20200.037.96518770
AAAGGGG2400.037.84029870
TCGTATG1650.037.38056640
TTTACCA1351.1609039E-437.3731470
Produced by FastQC (version 0.11.5)