FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685413

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685413
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911949
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35020.3840127024647212No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA29970.32863679876835217No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25180.2761119316979349No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT21270.23323672705381548No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC21200.23246914026990545No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20200.22150361478547592No Hit
GTCTGGGACAGACTTCACTCTCGCCATCAGCAGCCTAGAGCCTGAAGATT20010.21942016494343433No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC19420.2129505049076209No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC19410.21284084965277664No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT18440.20220428993288No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGCAGGGCCAGTCAGAGTATTAGCAACTACTTAGCCTGGTA18300.20066911636505988No Hit
GTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGCAGGGCCAGTCAGAGTATTAGCAA18190.19946290856177265No Hit
GAGTATTAGCAACTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATC17250.18915531460640891No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16840.1846594491577928No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC16470.1806022047285539No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATGCCAGTGGCCCTGTTGGATGCATCATAGATGAGGAGCCTG16160.17720289182838075No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA16060.1761063392799378No Hit
CTACGCAGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAATTCCA15870.1740228894378962No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT15610.17117185281194452No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA15170.16634702159879555No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT14980.16426357175675393No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT14950.16393460599222104No Hit
GTTTATTACTGTCAGCAGCGTAGCAACTGGCCTACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTG14910.16349598497284387No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14710.16130287987595798No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA14260.15636839340796468No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTACCTTCGGCCAAGGGACAC14090.1545042540756117No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13120.14386769435571506No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTTAGTAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGG12980.14233252078789493No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA12900.14145527874914057No Hit
GACTTCACTCTCGCCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTG12870.14112631298460768No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGCGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12600.13816562110381173No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT12390.13586286075208154No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC12160.13334078989066275No Hit
GAAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTTGCAGATACACCGTGTTCTTGGAATTGTCTCTGGA11950.13103802953893254No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT11900.13048975326471107No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11860.13005113224533388No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC11770.12906423495173525No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11740.12873526918720235No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA11200.12281388542561042No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC11110.12182698813201177No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT11050.121169056602946No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC10960.12018215930934735No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTTTCCTGCAGGGCCAGTCA10850.11897595150606011No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACACAGTCTCCAGCCACCCTGTCTTTGTCTCCA10720.11755043319308425No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGTCACGTTCTGTCCGCTTTCGCTCCAG10630.1165635358994856No Hit
GTCGTGTCCCTTGGCCGAAGGTAGGCCAGTTGCTACGCTGCTGACAGTAATAAACTGCAAAATCTTCAGGCTCTA10340.11338353350900106No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT10210.11195801519602522No Hit
GCGTAGCAACTGGCCTACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTT9890.10844904704100777No Hit
GTGTGAGGTGCAGCTGGTGGAATCTGGGGGAGGCTTGATTCAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGC9740.10680421821834335No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGCCAGGTT9720.10658490770865477No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAA9620.10548835516021181No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA9530.10450145786661316No Hit
ACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCAT9480.10395318159239167No Hit
GGGTTCCCTGGCCCCAATAGTCAAAGTTGTCCAGCCACTTCTGCACACCTCCCGCACAGAAATATACGGCCGTGT9340.10241800802457154No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9280.10176007649550578No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9190.10077317920190712No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCGCCATCAGCAGCC9130.10011524767284136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCCA352.0630892E-4127.3044370
GGCAATT650.002414882668.5485470
AATGAAT10800.061.88409870
TAGGCAA1604.957583E-755.69568670
TATTCTA850.006986377852.41946870
TACTCAT850.006986377852.41946870
AATGGTA2301.9954314E-951.65976770
CAGCCAT11500.046.4937970
GGCGTCA1653.876422E-545.0066170
GCAGCGT16550.044.87064470
GTATCAA15750.039.496951
GGTATCA5950.039.420771
GACAGGT2000.039.0922621
CTCGTAT1553.516834E-838.32821370
GAGCAAA16300.037.3582570
AAGCGGT8300.035.7883970
GTGTTCA10150.035.1184670
CTTCCAT3406.293703E-834.94631270
CTTTTAA5151.8189894E-1234.6070370
AAGGACA17900.033.18923670
Produced by FastQC (version 0.11.5)