FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148541
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACAGTGGCCGATCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCA71110.6191333178354104No Hit
CTGTCTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAT48330.42079473000963835No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT39720.34583005743808887No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC35260.30699818291205977No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA30230.2632034903412242No Hit
GTAGTATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA27720.24134967754742756No Hit
GTCTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCC25370.2208889364855064No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT24400.21244343911101127No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24240.2110503673791358No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG23870.2078288889991737No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22490.19581364531174766No Hit
CTCTTGCAGGGCCAGTCAGAGTGTGAGCGGCGAGTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGC20640.1797062534119374No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGAGTCTGGGACAGAATTCACT20460.17813904771357747No Hit
GTCAGAGTGTGAGCGGCGAGTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG20450.1780519807303353No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20310.1768330429649442No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19810.17247969380283334No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG18650.1623799237467361No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTGAGCGGCGAGTTAGGCTGGTA18490.1609868520148606No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18270.1590713783835318No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTGAGCGG18080.15741710570192968No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG18050.157155904752203No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18040.1570688377689608No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17990.1566335028527497No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA17530.15262842162360768No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17500.15236722067388103No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17360.15114828290849No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTA17150.1493198762604034No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC16620.14470532614856588No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGA16160.14070024491942384No Hit
CATCTATGGTGTTTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGAGTCTGGGACAGAATTCA15930.13869770430485284No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT15830.1378270344724307No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAGACAG15500.13495382402543749No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTG15500.13495382402543749No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG14490.1261600587179735No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14410.12546352285203577No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14240.12398338413691806No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT14210.1237221831871914No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT14200.12363511620394918No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA14140.12311271430449587No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13980.12171964257262038No Hit
TCTATGGTGTTTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGAGTCTGGGACAGAATTCAC13950.12145844162289375No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13090.11397068106406302No Hit
GAGTGTGAGCGGCGAGTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGTTTC13010.11327414519812529No Hit
GTTTATTACTGTCAGCAGTATAGACAGTGGCCGATCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAA13000.11318707821488307No Hit
GTCCCAGACTCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGAAACACCATAGATGAGGAGCC12780.11127160458355427No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGCCTAACTCGCCGCTCACACTCTGAC12670.11031386776788987No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGCCTTTTT12620.10987853285167878No Hit
GTATACTACTGTGCCAAAAAACTTACAATGACGGACTACTCTTACTACAT12540.10918199698574105No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12300.10709238938792782No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12140.10569931765605234No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC12080.10517691575659903No Hit
CTCCTGTGCAGCCTCTGGGTTCAGTTTTAGCAGCTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGGG12040.10482864782363015No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGACTCACTGCCA11720.10204250435987919No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11530.10038823167827705No Hit
GGATACCAGTGGCCCTGGTGGAAACACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGC11510.10021409771179261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG11650.053.45374370
TGTTAGA9600.051.89467670
TCGTATG2400.051.40995840
AATGGTA3100.050.2206570
CGTATGC2500.049.44194441
TCGGGTT305.702337E-446.95686352
TATCTCG2750.045.69858636
CGAGACG2950.044.2253923
TATACAC3050.043.790053
ACTCCTA3050.041.97519731
GCGTAGA21850.041.32573770
ATGCCGT3100.041.2181644
GTATGCC3100.041.0720542
ACGAGAC3200.040.7578422
ATCTCGT3050.040.17722737
CGGACTC3200.039.8605128
GCCGTCT3400.039.79546746
CCTATCT3150.039.7635134
ACTTCCA4200.039.538870
TGCCGTC3250.039.40450345
Produced by FastQC (version 0.11.5)