FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1148541
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACAGTGGCCGATCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAACTGTGGCTGCACCAT64890.5649776542587509No Hit
CTGTCTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATT46390.4039037352606481No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT40050.34870326788508205No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA34100.29689841285596247No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27550.23986953883230988No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26550.23116284050808808No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGGTGAGGAAGATGCTGGCAAAGGATGG26170.22785429514488384No Hit
GTCTATACTGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCC24850.21636145335691107No Hit
GTAGTATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGAGTGTTCTTGGAATTGTCTCTGGA24740.21540371654124668No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT24340.21192103721155794No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC22560.19642311419444322No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC22430.19529124341229437No Hit
GTCAGAGTGTGAGCGGCGAGTTAGGCTGGTACCAGCAGAAACCTGGCCAG21140.1840596025740483No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC20010.1742210334676777No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19920.17343743061849773No Hit
CTCTTGCAGGGCCAGTCAGAGTGTGAGCGGCGAGTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT19400.1689099474899024No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGAGTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCT18780.16351179452888492No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA18760.16333766056240046No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA18600.16194458883052498No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC18160.15811364156786742No Hit
GTCTGGGACAGAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT17830.1552404311208742No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA17420.1516706848079433No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGT17260.1502776130760678No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGGGTCAGTAGCAGGTGCCAGCTGTGTC17210.14984227815985673No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTGAGCG17130.14914574229391897No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGGGCCAGTCAGAGTGTGAGCGGCGAGTTAGGCTGGTA17110.14897160832743453No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16180.14087437888590829No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16120.140351976986455No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT15900.1384365033551262No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGA15610.13591156084110187No Hit
GTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTG15070.13120994374602213No Hit
CATCTATGGTGTTTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGAGTCTGGGACAGAATTCA15070.13120994374602213No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA14960.13025220693035774No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC14520.12642125966770015No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT14420.12555058983527798No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT14270.12424458508664472No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAGACAGTG14150.12319978128773809No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCA14000.12189377653910483No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC13970.12163257558937816No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT13560.11806282927644726No Hit
GAGTGTGAGCGGCGAGTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGTTTC12870.11205520743273421No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCTTGCAGG12860.11196814044949202No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12760.11109747061706983No Hit
GTTTATTACTGTCAGCAGTATAGACAGTGGCCGATCACCTTCGGCCAAGGGACACGACTGGAAATTAAACGAA12310.10717945637117005No Hit
GTCCCAGACTCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGAAACACCATAGATGAGGAGCCTG12270.10683118843820116No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA12190.10613465257226341No Hit
CTCCTGTGCAGCCTCTGGGTTCAGTTTTAGCAGCTATGCCATGACCTGGGTCCGCCAGGCTCCAGGGAAGGG12140.10569931765605234No Hit
GAGTTAGGCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGTTTCCACCAGGGCCA12030.10474158084038793No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATTTCCAGTCGTGTCCCTTGGCCGAAGGTGATCGGCCA11630.10125890151069922No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11620.101171834527457No Hit
TCTATGGTGTTTCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGAGTCTGGGACAGAATTCACT11570.10073649961124592No Hit
GAGTGAATTCTGTCCCAGACTCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGTGGAAACACCATA11550.10056236564476148No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGCCTAACTCGCCGCTCACACTCTGACTG11500.10012703072855039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATTA1101.8189894E-12110.19015570
ATCTCAT700.002994171364.9334970
GTAGTTT2003.313835E-853.0290170
AATGGTA2751.4733814E-1049.58556770
GCGTCAT11350.049.391470
GCGGGAA15200.047.84572670
CCTTAAA1450.001010252741.79626570
AAACTCA2254.5294746E-640.40305770
ACCGGAT350.001340684739.47103544
GTGGCAT4650.039.0997370
GTATCAA23800.038.721741
ATGGCAA2356.105847E-638.68377770
AAGTTTT9000.037.03613770
GGTATCA9000.035.9875221
ACCGGTA6350.035.79010870
AATACGT755.398215E-835.58860410
GCAGTAT14050.035.58632370
GAGCAAA19250.035.41826670
AAAGTCA3008.010629E-735.35267670
ACTTCCA4303.0195224E-1035.23522670
Produced by FastQC (version 0.11.5)