FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181277
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATACTTACGTCCTGATGTACCCTTTTGGCCAGGGGACCAAGCTGG13130.724305896500935No Hit
GTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC11100.612322578153875No Hit
GAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCG8010.4418652118029314No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC7750.427522520783111No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT7150.39442400304506364No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT6260.3453278684002935No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5740.3166424863606525No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC5500.30340307926543353No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4850.2675463517158823No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4820.26589142582897995No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC4690.25872008031906973No Hit
GTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTA4330.23886096967624132No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG4260.2349994759401358No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG4240.23389619201553422No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT4240.23389619201553422No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG4220.23279290809093264No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT4170.23003469827942874No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG4110.226724846505624No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGG4050.22341499473181925No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC3870.2134854394104051No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACA3870.2134854394104051No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT3740.20631409390049482No Hit
CTAATACTCTGACTGGCCCGGCAAGTGATGCTGACTCTGTCTCCTACAGA3720.20521080997589325No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA3600.19859110642828376No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3560.19638453857908064No Hit
GTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAA3560.19638453857908064No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3400.187558267182268No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC3350.18480005737076408No Hit
GTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT3250.17928363774775619No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATG3220.17762871186085383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3190.17597378597395147No Hit
GATGTACCCTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGG3180.17542214401165068No Hit
GTAATACATGGCGGTGTCCGACGCCTTCAGGCTGCTCCACTGCAGGTAGG3160.1743188600870491No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC3110.17156065027554515No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG2990.16494094672793572No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGG2930.16163109495413097No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGACTGGCCCGGCAAGTGA2920.16107945299183019No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC2900.15997616906722859No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA2890.15942452710492783No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC2820.1555630333688223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA2790.15390810748191994No Hit
GGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAGCAGCAGGAGCCCCAGGAG2780.15335646551961915No Hit
GGGTACATCAGGACGTAAGTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGA2770.15280482355731836No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA2770.15280482355731836No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT2770.15280482355731836No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC2760.15225318159501755No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGGGTACATCAGGACGTAAGTATTATAG2740.15114989767041598No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACCAGCTACTAATACTCTGA2700.14894332982121283No Hit
GGCCAAAAGGGTACATCAGGACGTAAGTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG2600.14342691019820494No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCCAGATGACCCAGTCTCCTTCCACC2570.1417719843113026No Hit
GTATTACTGTGCGAGACAACCGGAAGGTGGCGCAAATCGCCCTTTTGACTACTGGGGCCAGGGAACCCTGGTCAC2570.1417719843113026No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC2540.14011705842440023No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAATGTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGA2520.13901377449979865No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCAGCATCACTTGCCGGGCCAGTCAGAGTATTAGTAG2470.1362555646882947No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC2440.13460063880139234No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG2430.13404899683909155No Hit
ATGTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTC2390.1318424289898884No Hit
TCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAACATTCAGCGGCAGTGGATCTGGGACAGAATTCAC2380.13129078702758762No Hit
CTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAACATTCAGCGGCAGTGGATCTGGGACAGAA2360.13018750310298605No Hit
CCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCATCAACATTCAGCGGCAGTGGATC2350.12963586114068526No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAGGCCAACC2330.12853257721608366No Hit
GGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGGCGTCTAGTTTAGAAAGTGGGGTCCCA2310.1274292932914821No Hit
CAGTAATACATGGCGGTGTCCGACGCCTTCAGGCTGCTCCACTGCAGGTAGGCGGTACTGATGGACTTGTCGG2310.1274292932914821No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC2310.1274292932914821No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA2240.12356779955537658No Hit
GTCTCCTACAGATGCAGACAGGGTGGAAGGAGACTGGGTCATCTGGATGTCACATTTGGCACCTGGGAGCCAGAG2240.12356779955537658No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT2220.12246451563077501No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC2210.12191287366847421No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA2150.11860302189466948No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA2150.11860302189466948No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC2130.1174997379700679No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA2100.11584481208316555No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA2090.11529317012086476No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT2070.11418988619626318No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA2070.11418988619626318No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC2020.11143167638475923No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG2020.11143167638475923No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG2010.11088003442245845No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC1990.10977675049785686No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT1980.10922510853555607No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT1950.10757018264865373No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCC1940.10701854068635294No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG1930.10646689872405216No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCT1920.10591525676175136No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT1900.10481197283714978No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC1900.10481197283714978No Hit
TTCTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTT1890.10426033087484898No Hit
GTCAGAGTATTAGTAGCTGGTTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAACTCCTGATCTATAAGG1880.1037086889125482No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG1870.10315704695024741No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA1850.10205376302564584No Hit
GTATCAACGCAGAGTACGGGAGTCAGACCCAGTCAGGACACAGCATGGAC1850.10205376302564584No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG1840.10150212106334504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGCG701.8848649E-458.6688470
CCCCGAG550.005359735356.0020770
TAGGCAA1104.7148205E-756.0020770
CGAGTTC200.00630080353.80210560
GTATGCC453.1635864E-853.3265442
CGTATGC453.263449E-853.11700441
TCGTATG453.270725E-853.102140
GATGAAT200.007311680451.81622346
GCCGTCT408.1529834E-751.81622346
CAGTCAA200.00780823750.9625138
ATCTCGT409.1614675E-750.9482837
TACGTTT200.00801723850.6232332
ACCCGTG200.00808782250.5111429
TCACCCG200.0081144150.46923427
CACCCGT200.0081144150.46923428
GCTTATG200.0081677850.385631
CGAACCC200.00819456250.3439310
TCGAACC200.00819456250.343939
AATCGAA200.00819456250.343937
CTCGCTA352.6442187E-548.01282523
Produced by FastQC (version 0.11.5)