FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574255
Sequences flagged as poor quality0
Sequence length18-76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT15640.27235287459403923No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAA9890.17222314128740715No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACAAACGAACCTTTAATAGCGGCTGCAC8230.14331612262844903No Hit
CTTTCAAGGGTGGCTGCTTCTAAGCCAACCTCCTGGTTGTCTCAGCAACT8050.1401816266292849No Hit
GTATCAACGCAGAGTACGGGAATATCAACCCGTTGTCCATCGACTACGCCTGTCGGCCTCGCCTTAGGTCCCGA7500.13060399996517227No Hit
CCCCTACCCATCCAGCAAAACTGAATGCCACGGTTTCGGCGGTGTACTTG6590.11475738130273137No Hit
GACTTACCCAGGGCAGATTAGCTTGACCCTGGAACCCTTAGTCATCCGGCGGACGGGTTTCTCACCCGTCTTTCG6550.11406082663625045No Hit
GTTAGGGTACCGCGGCCGTTAAACATGTGTCACTGGGCAGGCGGTGCCTCTAATACTGGTGATGCTAGAGGTGA6480.11284185596990885No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG6130.10674700263820079No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCT6060.10552803197185918No Hit
GGGTAGGGGAGCGTCGTTCACGAGGTGAAGCCAGCGGGTAACTTCTGGTGGATTGTGGACGAGTGAGAATGCAGG6060.10552803197185918No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC6010.10465733863875805No Hit
GATCAGTACCGTGAGGGAATGGTGAAAAGTACCCCGGGAGGGGAGTGAAA5980.10413492263889736No Hit
GTATCAACGCAGAGTACGGGGGAAGGCGCTTTGTGAAGTAGGCCTTATTT5940.10343836797241644No Hit
GGGCAGATTAGCTTGACCCTGGAACCCTTAGTCATCCGGCGGACGGGTTTCTCACCCGTCTTTCGCTACTCATGC5910.10291595197255575No Hit
CCCTTGAAAGAGTGCGTAATAGCTCACTGGTCAAGTGATTCCGCGCCGACAATGTAGCGGGGCTCAAGTACACCG5870.10221939730607484No Hit
CTCTTACACGCGCTTCAACCTGGCCATGGGTAGATCACTCCGCTTCGGGT5760.1003038719732523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2800.071.7678370
TTAACGA550.005858093554.80452770
GGTATCA14800.040.4134061
CTAGATC2650.039.2725533
TTATACA2900.038.7224272
ACTAGAT3000.037.9605632
GTATCAA33700.037.850011
CGTATGC3100.036.70577641
TATACAC3150.036.6977273
CGCCCTA4400.036.5363570
ACCGTAC3100.036.35717427
ACGAGAC3100.036.23367322
TACTAGA3150.034.9968831
GTACTAG3350.034.8282930
GCCGTCT3450.034.63960646
AGACCGT3250.034.6065625
GTGGTAT3350.034.5248531
CTCGTAT3100.034.2167839
TAGATCT3100.033.66705334
ATCTCGT3350.032.4127537
Produced by FastQC (version 0.11.5)