FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences980666
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACA37400.38137347476102973No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT26550.2707343784734048No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG26280.26798114750587865No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25890.2640042583305631No Hit
GAGTTACAGTACCCCTCTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAACTGTGGCTGCACCA23240.2369818062418805No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGG20250.2064923225644613No Hit
CAGTAATACACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTGCAGATACAGTGAGTTCTTGGCGTTGTCTC20150.20547260739130346No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT17170.17508509523120003No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16610.16937469026151616No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC16420.16743723143251626No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16190.16509188653425325No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG16110.16427611439572698No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15790.16101302584162192No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15430.1573420512182537No Hit
AGCCTGGAGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTC15370.15673022211435902No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14890.15183558928320143No Hit
GATCTATGCTGCATCCAGTTTGCAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGATTTCA14860.15152967473125406No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA14840.1513257316966225No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG14660.14949024438493838No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG14390.14673701341741224No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT14340.14622715583083334No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13990.1426581527247809No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13800.140720693895781No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAGCTATTTAAATTGGTATCAGCAGAAA13710.13980295023993897No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13460.1372536623070444No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGACTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGG13420.13684577623778127No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC13370.13633591865120234No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC13180.13439845982220244No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG13170.13429648830488666No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13150.13409254527025513No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12920.13174720037199208No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT12910.1316452288546763No Hit
GTATTACTGTGCGAGGCACAGGAGCCAAAAATCGTCTTCTGCTTTTGATATCTGGGGCCAAGGGACAATGGTCAC12770.13021762761225533No Hit
GCATAGATCAGGAGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACCAATTTAAATAGCTGCTAATGCTCTGACT12190.12430327960793991No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC12090.12328356443478208No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12050.12287567836551895No Hit
CTGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGCTGATGGTGAGAGTGAAATCTGTC11830.1206323049845717No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11440.11665541580925616No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG11400.11624752973999303No Hit
CCCTGAAGAGACGGTGACCATTGTCCCTTGGCCCCAGATATCAAAAGCAGAAGACGATTTTTGGCTCCTGTGCC11280.11502387153220363No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC11260.11481992849757207No Hit
GAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGT11120.11339232725515108No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCAGAGGGGTA11000.11216866904736168No Hit
GTATCAACGCAGAGTACGGGAGCTCTGGGAGAAGAGCCCCAGCCCCAGAATTCCCAGGAGTTTCCATTCGGTGA10940.11155683994346699No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTT10940.11155683994346699No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCAGATGACCCAGTC10900.11114895387420386No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAAGCAGAAGACGATTTTTGGCTCCTGTGCCTCGCACAGTAATACACGG10840.11053712477030915No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATG10760.10972135263178288No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT10390.10594840649109889No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACA10360.10564249193915157No Hit
GGATGGGGGGATGGCGAAGACCCGGATGGCTGTGTCTTGATCGGGGACAC10270.1047247482833095No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCC10250.10452080524867793No Hit
GTGTATTACTGTGCGAGGCACAGGAGCCAAAAATCGTCTTCTGCTTTTGA10240.10441883373136217No Hit
CTACTACATGAGCTGGATCCGCCAGGCTCCAGGGAAGGGGCTGGAGTGGGTTTCATACATTAGTAGTAGTAGTAG10160.10360306159283589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG15000.055.50158770
CTACGAA200.00763995651.2613435
CGACCGA200.00775228751.07243328
TCGACCG200.00776327351.05414627
TATACAC3800.048.2043083
GTGAGCG5100.048.01175370
CTTATAC4050.046.068651
CGTATGC4100.044.390841
ACAGACG7600.043.81704370
CTCTTAT1250.043.4217951
AATGGTA3450.042.5843470
TCGTATG4250.041.97922540
TTATACA4450.041.925512
ACGAGAC4450.041.24314522
CGAGACA4600.040.65165323
ATGCCGT4500.040.51815444
ACATGCG4450.039.77251427
ATCTCGT4050.039.72019637
CACGAGA4650.039.42895521
TGCGCAG4500.039.37895630
Produced by FastQC (version 0.11.5)