FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1149392
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT61200.5324554199089605No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58080.5053106337959548No Hit
GAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAA47070.4095208597240976No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGAAATGGATACAACTATT45790.3983845372161978No Hit
CTACAAACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACG42830.3726317914166794No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA42070.3660195999276139No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC40540.35270821442988987No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT39160.3407018667260604No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA38090.3313925971296129No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36190.31486211840694905No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC34770.3025077606247477No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG32340.2813661483636566No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG32020.27858206773668165No Hit
GGTCTAGTCAGAGCCTCCTGCATAGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC31850.2771030249036012No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC31460.2737099266394755No Hit
GGCCAAAACTGTACGGAGTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGA31260.2719698762476161No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG29970.26074655122012336No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTC29930.2603985411417515No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29460.25630942272088203No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28190.24526010273257515No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGG27770.24160599690967052No Hit
GTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG27270.23725587093002212No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27250.2370818658908362No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC26710.23238372983281597No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCA25830.22472750810863482No Hit
GATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC25500.2218564249620669No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT25390.22089939724654425No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTGTACGGAGTTTG24650.21446121079666466No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG24020.20898005206230774No Hit
GTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGA23510.2045429235630664No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTGCATAGAAA23100.20097582025975472No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22800.19836574467196572No Hit
GTGTAAAATCTGTGCCTGATCCACTGCCACTGAACCTGTCAGGGACCCCGGAGGCCCGATTAGAACCCACATAGA22380.19471163884906106No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA22300.19401561869231732No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG21860.19018750783022675No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG21670.1885344599579604No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC21360.1858373818505784No Hit
GATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAAT21350.18575037933098543No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21210.18453234405668387No Hit
GTCAGGGACCCCGGAGGCCCGATTAGAACCCACATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA21000.18270529114523157No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC20820.18113924579255816No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT19940.173483024068377No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATGTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAG19570.17026393084343722No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA19510.16974191572587943No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC19200.16704483761849745No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG18890.16434775951111544No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18800.16356473683477873No Hit
GTTCAGTGGCAGTGGATCAGGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTA18670.16243370408007016No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAATCTGTGCCTGATCCACTGCCA18530.16121566880576862No Hit
GTTTATTACTGCATGCAAGCTCTACAAACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAA18520.16112866628617564No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA18200.1583445856592007No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCA17650.15355944708158747No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCAC17360.15103637401339143No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17200.14964433369990393No Hit
TTATTACTGCATGCAAGCTCTACAAACTCCGTACAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTG17090.14868730598438132No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCCCCACTGGATCCAGAGACCCAGAGCA16900.14703425811211493No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTT16790.1460772303965923No Hit
GACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAA16710.14538121023984854No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC16560.14407617244595403No Hit
ATGTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGT16540.1439021674067681No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAG16310.14190110945612985No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTTCTATGC16220.14111808677979312No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTG16020.1393780363879338No Hit
GCTCCTGATCTATGTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTGGCAGTGGATCAGGCACAGA15930.13859501371159708No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15720.13676796080014478No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTT15460.13450589529072762No Hit
GTGTATTACTGTGCGAGAGGGAGGGGGGGCGGTGACTACGGTTATTACTGGGGCCAGGGAACCCTGGTCACCG15390.13389687765357686No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATAT15330.13337486253601905No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC15320.13328786001642606No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15250.1326788423792753No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTA15220.1324178348204964No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC15200.13224382978131047No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGGCACCTTCAGCAG15110.13146080710497376No Hit
GAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATGTGGGT14960.13015576931107925No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC14800.12876372899759178No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGG14790.1286767264779988No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14630.12728468616451133No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT14410.12537063073346605No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGG14400.12528362821387307No Hit
GAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCGTACAGTTTTGGCCAGGGGA14340.12476161309631527No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT14340.12476161309631527No Hit
AGTCAGAGCCTCCTGCATAGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG14290.12432660049835043No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC14200.12354357782201372No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14150.1231085652240489No Hit
GTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTGCTGATTTTCAGTGTAAAA14150.1231085652240489No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC14020.12197753246934032No Hit
GGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATGTGGGTTCTAA13900.1209335022342247No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA13630.11858443420521458No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13610.11841042916602865No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA13520.11762740648969194No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA13450.11701838885254116No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT13360.11623536617620446No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13240.11519133594108885No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGT13150.11440831326475213No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13120.11414730570597324No Hit
GCATAGAAATGGATACAACTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATG12960.11275526539248576No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12860.11188524019655609No Hit
GGCACAGATTTTACACTGAAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA12780.11118922003981237No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCT12680.11031919484388267No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC12660.11014518980469673No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGG12550.1091881620891741No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC12460.1084051394128374No Hit
GTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAC12410.10797012681487256No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12300.10701309909934992No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG12260.10666508902097804No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATGTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGT12170.10588206634464135No Hit
GATCTATGTGGGTTCTAATCGGGCCTCCGGGGTCCCTGACAGGTTCAGTG12150.10570806130545542No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12130.10553405626626948No Hit
CTCCACAGCTCCTGATCTATGTGGGTTCTAATCGGGCCTCCGGGGTCCCT12010.10449002603115387No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG11900.10353299831563122No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC11620.10109692776702814No Hit
GGCTTCTGCAGGTACCAATCCAAATAGTTGTATCCATTTCTATGCAGGAGGCTCTGACTAGACCTGCAGGAGA11540.1004009076102844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)