FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459188
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT13990.30466824045924545No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT12000.2613308710157931No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC11960.2604597681124071No Hit
GTATTACTGTCACCAGTATGGTTTCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTG10000.21777572584649424No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA9370.20405585511816512No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT8370.1822782825335157No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG8120.17683388938735334No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG7890.17182504769288398No Hit
CAATAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGTTCCATGTAGACTGTGCTCGTGGATGTGTCTG7760.16899396325687954No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT7760.16899396325687954No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT7490.1631140186590242No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA7450.1622429157556382No Hit
GTATTATTGTGCGAGAGGGGGTGAGCAGTGGCTGGTGTCTTTCTTTCTTTACTGGGGCCAGGGAACCCTGGTCAC7350.1600651584971733No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7280.1585407284162478No Hit
CAGTAATACACGGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCTGAGACCCACTGCCG7220.15723407406116885No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7150.1557096439802434No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6930.1509185780116205No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6700.14590973631715115No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6590.1435142033328397No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC6540.14242532470360725No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG6520.14198977325191425No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC6290.13698093155744487No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6290.13698093155744487No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT6280.1367631558315984No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6280.1367631558315984No Hit
GACTATGAGTTACACCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG6220.13545650147651941No Hit
CTCCTGCAGGGCCAGTCAGACTATGAGTTACACCTACTTAGCCTGGTACC6120.13327874421805447No Hit
CCTTTATGGTACATCCAGCAGAGCCACTGGCATCCCCGACAGGTTCAGCGGCAGTGGGTCTCAGACAGACTTCA6050.131754314137129No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT5950.12957655687866407No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA5930.1291410054269711No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5860.12761657534604562No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT5790.12609214526512016No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCA5730.1247854909100412No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCG5670.12347883655496224No Hit
GTCTACATGGAACTGAGGAGCCTGACATCTGACGACACGGCCGTGTATTATTGTGCGAGAGGGGGTGAGCAG5590.12173663074819029No Hit
GTCTCAGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACTTTGCCGTGTATTACTGTCACCAGTA5490.11955887348972533No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC5490.11955887348972533No Hit
ATAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGTTCCATGTAGACTGTGCTCGTGGATGTGTCTGTGG5410.11781666768295339No Hit
CCTTGGGAGAATCCCCTAGATCACAGCTCCTCACCATGGACTGGACCTGGAGCATCCTTTTCTTGGTGGCAGCAG5340.11629223760202792No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC5300.11542113469864194No Hit
GGTTACACCTTTACCAACTATGGAATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGA5240.11411448034356299No Hit
GTCTGGAGCTGAGATGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCCTCTGGTTACACCTTTACCAA5160.11237227453679104No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG5150.11215449881094453No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGA5090.11084784445586557No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC5060.11019451727832608No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATGAGTTACA5010.10910563864909362No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT4970.10823453574570766No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG4950.10779898429401466No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGAC4940.10758120856816816No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4930.10736343284232165No Hit
ATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGCCCACCATGCAGGAGAAGGTGTCCCCCTTCTTCCAG4870.1060567784872427No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA4850.1056212270355497No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC4840.10540345130970322No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTATGAGTTA4830.10518567558385672No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC4750.10344346977708477No Hit
GTGTATTACTGTCACCAGTATGGTTTCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAA4730.10300791832539179No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC4710.10257236687369879No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA150.002644869767.006533
TAGGCAA1405.16593E-1060.1391970
CAGTTCG5350.051.14641670
GGTATCA4150.049.2404021
TCGTATG3100.048.52004640
CGTATGC3150.047.8402641
AATGGTA1102.6992779E-547.83799770
CTTATAC3250.047.414851
AGGGGGG7150.047.10202470
ACGAGAC3150.046.8492422
ATCTCGT2700.046.60728537
TATACAC3250.046.389143
GAGACCG3200.046.1524424
GTATGCC3200.046.1200242
ATCTCCG3150.045.72963710
AGACCGA3250.045.47217625
CGTCTTC3150.045.2279948
CTTGAAA3500.044.79164557
CACGAGA3150.044.7099821
TTATACA3300.044.6661572
Produced by FastQC (version 0.11.5)