FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences459188
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29410.6404784097145396No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT10590.2306244936714374No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA10230.22278456754096362No Hit
GTATTACTGTCACCAGTATGGTTTCTCACCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGT9420.20514473374739758No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT8040.1750916835805814No Hit
CAGTAATACACGGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCTGAGACCCACTGCC7670.1670339817242611No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA7350.1600651584971733No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC7280.1585407284162478No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGAGACCA7270.15832295269040134No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC7110.1548385410768574No Hit
GTATTATTGTGCGAGAGGGGGTGAGCAGTGGCTGGTGTCTTTCTTTCTTTACTGGGGCCAGGGAACCCTGGTCAC6990.1522252323666995No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC6800.14808749357561607No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6630.14438530623622567No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG6540.14242532470360725No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6520.14198977325191425No Hit
CAATAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGTTCCATGTAGACTGTGCTCGTGGATGTGTCTGTG6450.1404653431709888No Hit
GTCTACATGGAACTGAGGAGCCTGACATCTGACGACACGGCCGTGTATTATTGTGCGAGAGGGGGTGAGCAGTG6400.1393764645417563No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC6300.1371987072832914No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG6140.13371429566974746No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC6120.13327874421805447No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5960.1297943326045106No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG5880.1280521267977386No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT5840.12718102389435265No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5580.12151885502234379No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA5510.11999442494141832No Hit
CCTTTATGGTACATCCAGCAGAGCCACTGGCATCCCCGACAGGTTCAGCGGCAGTGGGTCTCAGACAGACTTCA5500.11977664921557184No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC5420.11803444340879989No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5390.1173811162312604No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA5330.11607446187618144No Hit
GACTATGAGTTACACCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG5280.11498558324694896No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT5230.11389670461771648No Hit
ATAATACACGGCCGTGTCGTCAGATGTCAGGCTCCTCAGTTCCATGTAGACTGTGCTCGTGGATGTGTCTGTGGT5230.11389670461771648No Hit
GTCTCAGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGACT5160.11237227453679104No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5000.10888786292324712No Hit
CTCCTGCAGGGCCAGTCAGACTATGAGTTACACCTACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCCCCCAG4970.10823453574570766No Hit
GTCTGGAGCTGAGATGAAGAAGCCTGGGGCCTCAGTGAAGGTCTCCTGCAAGGCCTCTGGTTACACCTTTACCAA4960.10801676001986114No Hit
GTATCAACGCAGAGTACGGGACCCAAAAACCACACCCCTCCTTGGGAGAA4950.10779898429401466No Hit
GTAATACACGGCAAAGTCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCTGAGACCCACTGCC4900.10671010566478219No Hit
GTCTGAGACCCACTGCCGCTGAACCTGTCGGGGATGCCAGTGGCTCTGCTGGATGTACCATAAAGGAGGAGCCTG4890.10649232993893568No Hit
GGTTACACCTTTACCAACTATGGAATCAGCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGATG4750.10344346977708477No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC4730.10300791832539179No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT4660.10148348824446632No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG4660.10148348824446632No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGC4640.10104793679277332No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG4640.10104793679277332No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT4610.10039460961523385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)