FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences663459
Sequences flagged as poor quality0
Sequence length18-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT24760.37319563077748585No Hit
CCCTTGGACAGTCGGCCTCCATCTCCTGCGGGTCTAGTCAAAGCCTCGTA21870.32963604382486333No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA19630.29587359580622163No Hit
GTAATAGACGGCCGTGTCCTCAGATGTCAGGCCGCTCAGCTCCATGTAGG19400.2924069158757361No Hit
GTGTGTAGCTTGCATGCAGTAATAAAGCCCAGCATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGT16350.24643572549321058No Hit
GTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCT16190.24402412206330762No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT14150.21327617833204462No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC13830.20845297147223865No Hit
GTCTATTACTGTGCGAAAGGGGGGGATGTACTTTGGAGTGATTACCCTGGAGAAGACTCCTGGGGCCAGGGAACC13210.19910800818136462No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC13180.19865583253825783No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12850.19368190046408293No Hit
TTATTACTGCATGCAAGCTACACACTTTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGT12850.19368190046408293No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT12580.18961231967612166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC12230.1843369371732089No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTC12210.184035486744471No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAG12090.1822267841720438No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11850.17860937902718932No Hit
GGCCAAAACTGTACGGAAAGTGTGTAGCTTGCATGCAGTAATAAAGCCCAGCATCCTCAGCCTCCACCCTGCT11430.172278920023694No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA11110.16745571316388805No Hit
CGTATATAGTGAAGGAAATACCTCCTTGAATTGGTTTCAGCAGAGGCCAG10850.1635368575902957No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT10800.16278323151845103No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC10790.1626325063040821No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10530.15871365073048976No Hit
GTAATAAAGCCCAGCATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGGCTGACCCACTGCC10510.15841220030175188No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10250.15449334472815954No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCC9830.1481628857246642No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGTAAGGCTTCTGGAGGCACTTTCACCAA9760.14710780922408168No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA9740.14680635879534382No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCA9520.1434904040792272No Hit
GTCCAAGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATTACAACATCC9520.1434904040792272No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA9500.14318895365048934No Hit
GTCTGGGACCCCAGAGTCCCGATTAGAAACATGATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA9450.14243532757864466No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTAAT9190.1385164720050523No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACA9160.1380642963619455No Hit
GCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTAATGACTCAGT9100.1371599450757319No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGTTTGGTCCCCTGGCCAAAACTG9030.13610486857514933No Hit
CAGTAATAAAGCCCAGCATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGGCTGACCCACTGCC9000.13565269293204252No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGGCTAGGCACTGTGTGGCC8980.13535124250330466No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGCCACTGATTTCACACTGAAAATCAGCAGGGTGGAGGCTGAGGAT8770.132186013001557No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATCATGTTTCTAATCGGGACTCTGGGGTCCCAGACAGATTCAGCG8580.1293222339285472No Hit
GCCTACATGGAGCTGAGCGGCCTGACATCTGAGGACACGGCCGTCTATTA8570.12917150871417826No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAG8550.1288700582854404No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCCCAGGATCCAGTGGGGATGTTGTAATGACTCAGTCTCCACTCTCC8430.12706135571301316No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT8410.12675990528427528No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGGTGTCCAGTCCCAGGTGCA8350.12585555399806167No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG8330.1255541035693238No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC8320.12540337835495488No Hit
GTCTCCACTCTCCCTGCCCGTCACCCTTGGACAGTCGGCCTCCATCTCCT8210.12374540099689658No Hit
GGGTGGAGGCTGAGGATGCTGGGCTTTATTACTGCATGCAAGCTACACACTTTCCGTACAGTTTTGGCCAGGG8000.12058017149514891No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC7990.12042944628077998No Hit
CTCCCTGCCCGTCACCCTTGGACAGTCGGCCTCCATCTCCTGCGGGTCTAGTCAAAGCCTCGTATATAGTGAAG7930.11952509499456634No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG7800.11756566720777019No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA7620.11485261334912933No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG7560.11394826206291572No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT7540.11364681163417785No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7530.11349608641980892No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTATCTTTGGT7520.11334536120543998No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT7440.11213955949048848No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7400.11153665863301275No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCTGCGGTAATCGTGAGTCTGCCCTGGAACTTCTGTGCGTACTTTGTT7250.10927578041747871No Hit
CAGTAATAGACGGCCGTGTCCTCAGATGTCAGGCCGCTCAGCTCCATGTAGGCTGTGCTCGTGGATTCGTCTGCG7220.10882360477437189No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7120.10731635263068252No Hit
GCTACACACTTTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCAT7100.10701490220194466No Hit
CTCGTATATAGTGAAGGAAATACCTCCTTGAATTGGTTTCAGCAGAGGCC6960.10490474920077957No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCT6860.10339749705709018No Hit
CTTTCCGTACAGTTTTGGCCAGGGGACCAAACTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTT6820.10279459619961444No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAA6670.1005337179840804No Hit
GTAATAGACGGCCGTGTCCTCAGATGTCAGGCCGCTCAGCTCCATGTAGGCTGTGCTCGTGGATTCGTCTGCGGT6670.1005337179840804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTA8250.098.6867970
GTTATTA508.632142E-488.8181270
AGAGCAT4250.073.1443370
TAGTTTC150.002497603767.985245
TACTTCA800.005566430755.51132270
ACCAGGT1351.2050206E-554.82599670
GTGGTAT1950.054.0843771
TGGTATC1950.054.0476882
TTGCAAA1102.89867E-453.82916370
AATGGTA2054.8177753E-850.54689470
CCAAGCA900.00886844349.343470
CCTTGAA20000.044.40905870
TATAAGA405.4516076E-542.4971732
GAGCCAT8200.041.5206670
CTGCGGT9300.038.14721769
AATTTAT10100.038.1067870
GTATCAA12200.037.088271
ACTTCCA3203.7893187E-837.0075570
GGTATCA4850.036.4759251
AAGGACA11900.034.83063570
Produced by FastQC (version 0.11.5)