FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251719
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAG43540.3478416481654429No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAG39540.31588559413095113No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC37710.3012656994101711No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG37370.29854943481723933No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGTCCCG35220.28137305577370003No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTTTGATGTACACTGGTACCAGCACCTTCCAGGAGCAGC34920.2789763517211131No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC34390.27474217456154293No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAG31890.2547696407899856No Hit
GACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATATTCACTCAGG31730.2534913986286059No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC29470.2354362280991181No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATATTCACTCAGGCTGCTGTCATAGGA28280.2259293020238568No Hit
ACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATATTCACTCAGGC26790.2140256718960086No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAG26430.21114962703290435No Hit
GTAATATAGAGCCGTGTCCTCAATTCTCAGGCTGTTCATTTGCAGATGCA25850.20651599919790306No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTC25490.20363995433479876No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGAATATGTCTTCGG24480.19557105069108963No Hit
CTATATTACTGTGCCAAAAAAGGGAGAACATATTGTAGTCCTACCAGCTG24210.1934140170437614No Hit
GTCCTATGACAGCAGCCTGAGTGAATATGTCTTCGGAACTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGC23610.18862060893858765No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT23320.186303795021087No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC23160.18502555285970734No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC22600.1805517052948785No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCT21970.17551862678444602No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG21460.17144422989504834No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTTTGATGTACACTGGTACCAG21180.1692073061126339No Hit
GGGTTAGAGTGGGTGGCAGTTGTATCATCTGATGGAAATACTCAATCCTA20760.16585192043901226No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20200.16137807287418343No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGG19920.159141149091769No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTTTGA19490.15570587328306112No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGC19010.15187114679892214No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAGCAGCCCCCAAACTCCTCAT18680.14923477234107654No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGAATATGTCTTCGGAACTGGGACCGAGG18340.14651850774814476No Hit
CTCTAACCCCCTGCCTGGAGCCTGGCGGACCCAGTGCATAGCACAGCTAC18200.14540004585693753No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGG18130.14484081491133394No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGTCCCGTGATGGCCAGGGAGG18130.14484081491133394No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC18000.14380224315521295No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA17860.14268378126400574No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG17430.13924850545529788No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16260.12990135965020902No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA16010.1279041062730533No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACT15860.12670575424675987No Hit
GTTTGGGGGCTGCTCCTGGAAGGTGCTGGTACCAGTGTACATCAAAACGTGCCCCGATGTTGGAGCTGCTCCCAG15810.12630630357132872No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT15770.12598674303098378No Hit
GTGCTGGTACCAGTGTACATCAAAACGTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTC15700.12542751208538017No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA15620.12478839100469034No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15310.12231179681701725No Hit
GCCTCATCCTCAGCCTGGAGTCCCGTGATGGCCAGGGAGGCTGAGGTGCC15280.12207212641175856No Hit
GCCTGGAGTCCCGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC15280.12207212641175856No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGG15270.12199223627667231No Hit
ACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGAATATGTCTTCGGAA14930.11927597168374052No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACGGGACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTA14920.11919608154865428No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14640.11695915776623986No Hit
ATATTACTGTGCCAAAAAAGGGAGAACATATTGTAGTCCTACCAGCTGCCCGCCAGATCCTTTTGATATCTGGGG14260.11392333263296314No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14250.11384344249787692No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG14230.11368366222770446No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14210.11352388195753199No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC14150.11304454114701462No Hit
GGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAG14040.11216574966106611No Hit
GCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13740.10976904560847922No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTT13620.10881036398744447No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC13490.10777179223132348No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGA13360.1067332204752025No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAACTGGGTTTTC13300.10625387966468512No Hit
GAACTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCACT13280.10609409939451267No Hit
GCTCCAACATCGGGGCACGTTTTGATGTACACTGGTACCAGCACCTTCCA13260.1059343191243402No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTAGGTGGGAAGTTTCTGGCGGT13090.1045761868278743No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG13010.10393706574718448No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA12950.1034577249366671No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG12930.10329794466649464No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG12850.1026588235858048No Hit
CCATTGTCCCTTGGCCCCAGATATCAAAAGGATCTGGCGGGCAGCTGGTAGGACTACAATATGTTCTCCCTTTTT12810.10233926304545989No Hit
GCTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTC12660.10114091101916645No Hit
CAGTAATATAGAGCCGTGTCCTCAATTCTCAGGCTGTTCATTTGCAGATGCACCGTGTCCTTGGAATTGTCTC12600.10066157020864908No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCTGGCTGTGCCCAGCCA12550.10026211953321792No Hit
GTGCAGCGACAGTCGGGGGTGGCAGCATGGGGGAGGTGGGGGAACTGGGC12520.10002244912795924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAA608.619568E-568.818270
AGGGGGG2600.059.55420770
TCTGGAG12550.048.52916370
ATCTGGG14000.047.18961770
CCGTCGT306.323014E-445.9831847
CGCAAGG906.389231E-445.87879670
CGATTCA8900.042.91471570
GGTATCA8500.039.50321
CGTATGC2450.039.1785741
GTATCAA20800.038.808751
TCCCTAG1606.0865514E-638.7102370
TCGTATG2250.038.06567840
TTCTTAA1904.530848E-738.03110570
GTCGTAT451.0517609E-438.0165839
ATCTCGT1900.037.74043337
TTATACA3100.037.188772
ACGAGAC2850.036.89058322
AGACGTA2950.036.80002225
CACGAGA2950.036.7853221
TATACAC3150.036.599933
Produced by FastQC (version 0.11.5)