FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004685437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1251719
Sequences flagged as poor quality0
Sequence length18-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC38510.3076569102170695No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT38480.3074172398118108No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA37310.29807009400672196No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTTTGATGTACACTGGTACCAGCACCTTCCAGGAGCAGC35140.28073393469301017No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCT32650.260841291056539No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC31270.2498164524146394No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGG30650.24486326403929315No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGT29960.23935084471834334No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29450.23527644782894563No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGTCCCG29260.23375853526230728No Hit
GGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGCCCAGT26490.21162896784342172No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG25750.20571709784704076No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATATTCACTCAGGCTGCTGTCATAG24770.19788786460859026No Hit
GTAATATAGAGCCGTGTCCTCAATTCTCAGGCTGTTCATTTGCAGATGCACCGTGTCCTTGGAATTGTCTCTGGA24550.19613028163669322No Hit
GATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGAATATGTCTTCGGAACTGGGACCGAGGTCACCGTCCTA24100.19253522555781288No Hit
GTCCTATGACAGCAGCCTGAGTGAATATGTCTTCGGAACTGGGACCGAGGTCACCGTCCTAGGTCAGCCCAAGGC24070.1922955551525542No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22900.18294840934746537No Hit
GACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATATTCACTCAGG22750.18175005732117194No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC22610.1806315954299647No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTA22190.1772762097563431No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATATTCACTCA20900.1669703823302195No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC20780.16601170070918472No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC20710.16545246976358113No Hit
ACCTAGGACGGTGACCTCGGTCCCAGTTCCGAAGACATATTCACTCAGGC20690.16529268949340867No Hit
GGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTTTGATGTACACTGGTACCA20510.16385466706185653No Hit
CTATATTACTGTGCCAAAAAAGGGAGAACATATTGTAGTCCTACCAGCTGCCCGCCAGATCCTTTTGATATCTGG20400.162975875575908No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20010.15986016030754507No Hit
GGGTTAGAGTGGGTGGCAGTTGTATCATCTGATGGAAATACTCAATCCTA19550.1561852140935785No Hit
GATGTACACTGGTACCAGCACCTTCCAGGAGCAGCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCA19200.1533890593655605No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG18980.15163147639366345No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTT18800.1501934539621113No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGT18690.14931466247616276No Hit
CTCTAACCCCCTGCCTGGAGCCTGGCGGACCCAGTGCATAGCACAGCTACTGAAGGTGAATCCGGAGGCTGCACA18510.14787664004461065No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGG18340.14651850774814476No Hit
GTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCAGCTCCAACATCGGGGCACGTTTTGAT18310.14627883734288605No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT18200.14540004585693753No Hit
GGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGAATATGTCTTCGGAACTGGGACCGAGGT18010.14388213329029917No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC17910.1430832319394369No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTG17590.14052674761667755No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCT17330.13844960410443558No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAG16050.12822366681339822No Hit
GGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTC15780.12606663316607No Hit
GTGCAGGAGATGGTGACCCTCTGCCCTGGGGCCCCAGACACTGAGGGCGGCTGCGTCAGCACAGACTGGGCCCAG15150.12103355465563757No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAG15000.11983520262934413No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCACGGGACTCCAGGCTGAGGATG14830.11847707033287823No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC14810.11831729006270576No Hit
AGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGT14530.11608036628029134No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCT14400.11504179452417036No Hit
GTGCTGGTACCAGTGTACATCAAAACGTGCCCCGATGTTGGAGCTGCTCCCAGTGCAGGAGATGGTGACCCTCT14320.11440267344348053No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13940.11136684831020381No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA13790.11016849628391037No Hit
ATATTACTGTGCCAAAAAAGGGAGAACATATTGTAGTCCTACCAGCTGCCCGCCAGATCCTTTTGATATCTGGG13730.10968915547339297No Hit
GTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGGGAGCA13610.10873047385235825No Hit
ACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTCCTCGCTCACTGCACAGGGTCCTGGGC13550.10825113304184085No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGTCCCGTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGA13180.10529519804365038No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG13130.10489574736821923No Hit
GCCCCCAAACTCCTCATCTATGGTAACAGCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13100.10465607696296055No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTG13050.1042566262875294No Hit
ACTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGAATATGTCTTCGGAA13040.10417673615244316No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGACTTGCCGTCTGGGCACTGTGTGGCC12990.10377728547701201No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC12870.10281860385597726No Hit
GTTTGGGGGCTGCTCCTGGAAGGTGCTGGTACCAGTGTACATCAAAACGT12840.10257893345071857No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCGTCAAG12810.10233926304545989No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC12790.10217948277528742No Hit
CTTCAGCTGTGGGCACAAGAGGCAGCACTCAGGACAATCTCCAGCATGGCCTGGTCTCCTCTCCTCCTCACTCTC12590.10058168007356283No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGG12580.10050178993847661No Hit
GGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCACCATCTCCTGCACTGG12540.10018222939813168No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)