FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323427
Sequences flagged as poor quality0
Sequence length18-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20380.6301267364814935No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTA10960.33887090440810447No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9360.2894006993850235No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT9140.2825985461943499No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGTTA7530.23281915238987466No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTT7090.21921484600852742No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT6710.20746567231554572No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGC6490.20066351912487204No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG6450.19942676399929507No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG6290.19447974349698696No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAA5790.17902030442727418No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT5680.17561922783193734No Hit
GGACAATGGAAGTCTGCTGACAGTGATAAGTAGCAAAATCTTCAGGTTGGAGACTGCTGATGGTGAGAGTGAAAT5670.1753100390505431No Hit
CCATTGTCCCGTTCACTTTCGGCGGAGGGACCAGGCTGGCGATCAAACGA5470.16912626342265796No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGA5430.16788950829708094No Hit
GTAATATACGGCCGTGTCTGCGGCAGTCATAAAGTTCAGGTTCAGGAAAAAGTGGTTGTCAGACACATCTATT5210.16108735510640732No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC5100.1576862785110705No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT4860.15026574775760837No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT4800.14841061506924283No Hit
AGTCAGAACATTGCCACCTATTTGCATTGGTATCAGCAGAAACCAGGAAA4640.14346359456693475No Hit
GTGGGGTCCCATCAAGATTCAGTGGCAGTGGATATGGGACAGATTTCACTCTCACCATCAGCAGTCTCCAACCT4590.14191765065996345No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCC4550.14068089553438645No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTTCCATGGCTCGGCACAGCCCGGCAG4500.13913495162741515No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCTTCGCTGTGACCAGCATACTGCGCGT4460.13789819650183813No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGCCACAGTTC4420.1366614413762611No Hit
GTAGGAGACAGAGTGACCATCACTTGCCGGGCAAGTCAGAACATTGCCACCTATTTGCATTGGTATCAGCAGAAA4390.13573387503207834No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT4320.13356955356231853No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4250.13140523209255875No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCGGCCCGAGGTCCACCTGCTGCCGCC4210.13016847696698172No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT4120.12738577793443343No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA3790.117182548148423No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGGGATGCCCAAGTCAGGTACTTCTCGC3780.11687335936702874No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTAC3690.11409066033448041No Hit
GGTCACAGGAGCTGCCCCGCGAGAAGTACCTGACTTGGGCATCCCGGCAG3630.11223552764611489No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC3600.11130796130193212No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACT3490.1079068847065953No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC3420.10574256323683552No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC3360.10388743054846998No Hit
GATTTCACTCTCACCATCAGCAGTCTCCAACCTGAAGATTTTGCTACTTATCACTGTCAGCAGACTTCCATTGTC3350.10357824176707572No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT3320.10265067542289295No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA3290.1017231090787102No Hit
CACTTGCACTGCTGCCTACCCCGAGTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCCG3290.1017231090787102No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGAT3260.10079554273452743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAT150.0030325912192.5015670
CAGTGTT456.259655E-496.2507870
TATTCTA1002.3448665E-672.1880870
TTACCTA209.4072064E-567.60898628
GACGGAC200.00782483950.94435533
GAGCAAA7400.046.824770
GACGGCA1256.1408145E-446.2003770
ATTACCT306.996827E-445.03764727
AAGCGGT5450.045.03476370
GTATCAA12000.042.0334241
AACAAGT2502.7665374E-740.42532370
TCTTCAT5800.039.82790470
GACAGCC603.5483208E-739.2311971
GGAGCTT1500.001506260538.50031370
GCGGGAA4351.4551915E-1136.50891570
TATCAAC14200.035.7469522
ATCAACG14250.035.6215253
AAGGACA9000.035.2919570
TCAACGC14400.035.255924
AACGCAG14750.034.647286
Produced by FastQC (version 0.11.5)