FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004685440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004685440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1206364
Sequences flagged as poor quality0
Sequence length18-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAAATCTCAGGCTGTTCAGTTCCATGTAGG53240.44132616689490073No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTAT36120.29941211773560883No Hit
GTATGGTAGTACACCTCAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA33560.2781913253379577No Hit
GTCTATTACTGTGCGATAGGTTGGGGTGGCAACTGGGCCGGGGACTATTGGGGCCAGGGAACTCTGGTCACCG32690.27097957167156844No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31940.2647625426488191No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA27170.22522223806413322No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGGCTTCTGGAGACACTTTTAGCAC26990.22373015109867336No Hit
CTACCATACTGCTGACAGTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTC26760.2218235955316969No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAGTTGAGGGAGATGGGGTAGGTGGAGT25800.21386579838257774No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCT23840.19761862920312606No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22650.18775427648703044No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22320.18501878371702074No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22140.18352669675156089No Hit
GTCTTAGTAGTACCTACTTAGCCTGGTACCACCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCA22070.1829464407094376No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA22040.18269775954852763No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGTA21880.18137146002367446No Hit
GTAGTACACCTCAGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCT21520.17838728609275475No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21040.17440838751819515No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG20860.17291630055273532No Hit
GTGTACTACCATACTGCTGACAGTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAG20850.17283340683243198No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAG20240.1677768898939292No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20090.16653348408937932No Hit
CATCTATGGTGCATCCAGGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA20070.16636769664877266No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC20060.16628480292846937No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCC19750.16371509759906627No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCTGGATGCACCATAGATGAGGAGCC18470.15310470140024074No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18370.15227576419720748No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18180.1507007835114443No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAGTGTCCTGCCGGGCTGTGCCGAGCCA18140.150369208630231No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAGTACCTACTTAGCC17830.14779950330082794No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTTCTGCAGGGCCAGTCAGAGTCTTAGTAGTA17810.14763371586022128No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGG17730.1469705660977947No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGGTGGGTTTACCCGCCAAGCGG17520.14522979797142488No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG17240.1429087738029318No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17130.14199694287959522No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGGTGGTACCAGGCTAAGTAGGTACTACTAAGACTCTGA16910.14017328103292206No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC16650.13801804430503564No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16590.13752068198321568No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16380.13577991385684585No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT15980.13246416504471287No Hit
CTTTTAGCACCTCTGCTATCAGCTGGGTGCGACAGGCCCCTGGACAAGGACTTGAGTGGATGGGAGGGGTCATCC15910.13188390900258962No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCCGGTCGGTGCAGTGACAGTCGGGGG15820.13113786551985968No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA15720.13030892831682644No Hit
GTACTACCATACTGCTGACAGTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAG15680.12997735343561315No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAAC15580.1291484162325799No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA15450.12807079786863665No Hit
GTTCAGTTCCATGTAGGCTGTGCTCGTGTATTCGTCCGCGGTAATCGTGA15390.12757343554681672No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG15380.1274905418265134No Hit
CTTCTGCAGGGCCAGTCAGAGTCTTAGTAGTACCTACTTAGCCTGGTACC15350.12724186066560342No Hit
GAACTGAACAGCCTGAGATTTGAGGACACGGCCGTCTATTACTGTGCGAT15310.12691028578439012No Hit
GTTTATTACTGTCAGCAGTATGGTAGTACACCTCAGCTCACTTTCGGCGG15260.12649581718287348No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCTCCGCCGAAAGTGAGCTGAGGTGTACTACCATAC14830.12293138720983053No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGCCCTCCTGGATGCACCATAGA14640.12135640652406736No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC14630.12127351280376403No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCC14580.12085904420224743No Hit
TAGTAGTACCTACTTAGCCTGGTACCACCAGAAACCTGGCCAGGCTCCCA14460.11986431955860752No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAGGTCCCCGGAGGCATCCTGGCTGGG14380.11920116979618092No Hit
CTATTGGGGCCAGGGAACTCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCGCTGAGCC14260.11820644515254101No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT14140.11721172050890112No Hit
GCCTACATGGAACTGAACAGCCTGAGATTTGAGGACACGGCCGTCTATTACTGTGCGATAGGTTGGGGTGGCAA13990.11596831470435126No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT13940.11555384610283463No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACT13900.11522227122162133No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGG13830.11464201517949807No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT13570.11248677845161162No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC13520.11207230985009499No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGA13520.11207230985009499No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC13370.11082890404554512No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13350.11066311660493848No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCTGTTTATTACTGTCAGCAGTATGGTAGTACA13280.11008286056281522No Hit
CCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCAGGAGGGCCACTGGCATCCCAGACAGGT12820.10626974942886226No Hit
ATCCAGGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG12770.10585528082734563No Hit
GTCCCAGGTGCAGCTGGGGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCAAGG12760.10577238710704233No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC12740.10560659966643567No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12730.10552370594613236No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT12560.10411451270097583No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12390.10270531945581932No Hit
GTAATAAACAGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA12350.10237374457460602No Hit
GTTCCATGTAGGCTGTGCTCGTGTATTCGTCCGCGGTAATCGTGAGTCTGCCCTGGAACTTCTGTGCGTAGTT12200.10113033877005614No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTA304.4601693E-4104.89867470
CGCGGTA18550.054.85267370
CGTATGC2250.048.80691541
ATCTCGT2100.047.15003237
TCGTATG2350.046.6572240
CTTATAC2400.044.982461
TATATTA957.704193E-444.16786270
GGTATCA8950.043.3490221
GCCGTTA551.7539969E-742.93246515
GAGACTA2550.042.36805724
TCTCCGA2550.042.322411
TATACAC2700.041.221963
GACTAGG2650.040.80312326
AGACTAG2700.040.02755725
TGTTAGA8400.039.961470
TTATACA2800.039.74812
GATCTCG2500.039.54960336
CTCGTAT2600.039.48704539
ATGCCGT2800.039.36261444
CCGTTAA603.472378E-739.3547616
Produced by FastQC (version 0.11.5)